Carrier material in use for preparing dextran through method of enzyme immobilization

Abstract

A carrier material used in producing dextrans with a zyme immobilization method applies complex polymer glues as the immobilized carrier material composed of algae acid or with natural and or synthesized or water-soluble or semi-soluble polymers to immobilize dextran sucrose zymes and produce dextrans.

Description

1. Technical field [0001] The invention relates to a material in the bio-enzyme process, in particular to a carrier material in the immobilized enzyme process, specifically a carrier material for producing dextran by the enzyme-immobilized process. 2. Background technology [0002] Dextran, also known as dextran, is an excellent plasma substitute with antithrombotic and microcirculation improvement effects, and has been widely used in medical, food industry, biology and other fields. [0003] The traditional production process of dextran adopts fermentation technology, such as Zhang Zhaohui's "Preparation and Application of Dextran" ("Sichuan Chemical Industry and Corrosion Control" 2001, 4 (1), 50-52), Mariana Santos and others are studying the production of dextran Fermentation technology was also used ("Biochemical Engineering Journal" 2000, (4), 177-188). Although the process technology is relatively mature, the consumption of ethanol in the production is large, the pro...

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Problems solved by technology

However, in the production process, the bacteria and dextran are entangled with each other and cannot be separated, resulting in impurities such as miscellaneous proteins in the product

Adopt membrane separation technology in CN1415631A disclosed dextran production process, although this process has avoided the use of alcohol and some organic solvents, five-stage ultrafiltration membrane filtration, separation treatment, efficiency is not high in industrial production, and membrane technology method There are a series of problems such as concentration polarization and membrane pore pollution.

When alginate is used as the immobilized carrier material, the pores of the gel matrix are easily blocked by dextran, which reduces the material transport, and the gel strength of alginate is not high, and the alginate gel particles of the immobilized enzyme are easily broken. The use cycle is short, which affects the final output and quality of dextran and the control of the production process

Using porous cellulose as immobilized carrier material overcomes some shortcomings of alginate, but the immobilization rate is not high, and the final yield and quality of dextran are still unsatisfactory