Polysaccharose MF4 of mussel with enhancing immunity and anti-tumour activity

A thick-shelled mussel and polysaccharide technology, applied in the field of medicine, can solve problems such as roughness, and achieve the effect of improving function and promoting activity

Inactive Publication Date: 2005-02-23
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The front end of the shell is tapered, and the back end is wide and round; the shell surface is rough, covered with brown or chestnut brown shell skin (Wang Zhenrui, Chinese Zoology. Molluscs. Bivalvia: Mytilidae. 1st edition. Beijing .Beijing Science Press, 1997) Mussel meat is rich in nutrients and is an important source of high-grade protein and vitamin D; literature reports (Liu Zhifeng et al., China Mar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1. Preparation of thick shell mussel polysaccharide MF4

[0019] Select 16kg of fresh shellfish meat of thick-shelled mussels grown in the sea area of ​​Zhejiang, wash and crush with a mixer, heat and extract in 3 times the volume of boiling water for 4 hours, and the extract is precipitated with 2 times the volume of 95% ethanol. Wash with ethanol and acetone three times respectively, remove protein twice by Savager method, and freeze-dry to obtain crude mussel polysaccharide extract. The crude polysaccharide extract was separated with DEAE-cellulose-52, eluted with 0.05, 0.1, 0.2, 0.5, 1.0 mol / L NaCl in sequence, and the eluted fraction of 0.5 mol / L was collected and freeze-dried to obtain the MF fraction. MF was separated by Sephadex G-100, eluted with 0.05mol / L NaCl, and the first eluted peak was collected, and finally treated with HPLC, using 0.1M phosphate buffer as mobile phase, collected The first elution peak was separated and purified to obtain 217...

Embodiment 2

[0020] Example 2. Mussel thick shell polysaccharide MF4 enhances immune activity test in vivo and in vitro

[0021] 1. T, B lymphocyte proliferation response experiment

[0022] Add 5 mg·L of mitotic proconavalin A (ConA) as usual -1 To induce the proliferation of T lymphocytes, add mitogenic lipopolysaccharide (LPS) 10mg L -1 Induce the proliferation of B lymphocytes, the morphology and metabolism of the cells can undergo a series of changes, transform into mother cells, and differentiate and proliferate. The synthesis of intracellular protein and nucleic acid increases, and a variety of lymphokines are released. use 3 HTdR incorporation method to quantitatively determine the effect of samples on cell proliferation: adding 3 HTdR can be incorporated into newly synthesized DNA to quantify the strength of DNA synthesis in cells, thereby measuring cell proliferation. The radionuclide incorporation method is accurate and objective. The toxicity of the sample to cells was det...

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PUM

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Abstract

Glycan ingredient MF4 has increased immunological competence and anti-tumour functions, which is separated from sea-animal thick shell mussel. Its relative molecular weight is 113,000. It was analyzed and identified the physical properties and chemical structure through multiple spectra and methods, of which spectra are such as UV, IR, GC/MS,1HNMR, of which chemical methods are such as hydrolysis, acetylization, periodate oxidation, Smith degradation, methylation, sulfuric acid- carbazole reaction. Testing in vitro proves that MF4 promotes T,B lymph cells proliferation. Anti-tumour testing in vivo shows that small rat with subcutaneous inoculation is capable of inhibiting Lewis lung cancer and S-180 caruncle's growth, and has promotion functions for NK cell activity and lymph cell transfer of charged Lewis lung cance small rat. The thick shell mussel glycan MF4 is used to prepare new immonological strengthen pharmaceutics and anti-tumour drugs. And it's a new approach for developing Chinese sea biological sources.

Description

technical field [0001] The invention relates to the technical field of medicine, and is a new polysaccharide MF4 with immune-enhancing and anti-tumor activities isolated from the marine animal mussel thick shell. Background technique [0002] Thick-shelled mussel (Mytilus Coruscus) belongs to the Mytilidae family (Mytilidae). It is a warm-water species. In my country, it is mainly distributed in Dalian, Xiaochangshan, Liaoning Province; Qingdao, Tuoji Island, Shandong Province. Daqin Island, Penglai, Yantai, Weihai; Shengsi Islands, Zhoushan Islands, Zhujiajian, Yushan Islands, Xiadachen, Nanji Island in Zhejiang Province; Pingtan, Xiamen, Dongshan in Fujian Province. The shell is large, thick, and wedge-shaped; the adult body is about 100-200mm long, and the shell length is or more than twice the height, and about 2.5-3 times the width. The front end of the shell is tapered, and the back end is wide and round; the shell surface is rough, covered wi...

Claims

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Application Information

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IPC IPC(8): A61K31/722A61P35/00C08B37/08
Inventor 易杨华马明华李玲刘宝姝
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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