Bacteria growing inhibiting culture medium of white rot fungus degrading active dye in non-sterilized environment

Abstract

The present invention belongs to the field of applied microbiologic technology. The liquid culture medium is developed on the basis of available liquid culture medium for cultivating white rot fungus, through regulate C / N ratio and trace element Fe amount and by means of the characteristic of white rot fungus capable of growing optimally in acid environment. The developed liquid culture medium can suppress bacteria growth while the white rot fungus degrades active dye. Contrast test shows that the white rot fungus cultured in the liquid culture medium has decolorizing rate on brilliant red K-2BP under non-sterilizing environment similar to that in sterilizing environment, and no mass bacteria are found in the microscopic examination of the liquid culture medium. The present invention makes it possible to apply white rot fungus in treating dye containing waste water practically.

Description

technical field [0001] The invention belongs to the field of applied microorganisms, in particular to the development of a liquid culture medium for inhibiting bacterial growth when white-rot fungi are used to degrade active dyes in a non-sterile environment. Background technique [0002] Dye-containing wastewater is currently recognized as difficult-to-treat industrial wastewater at home and abroad. In recent years, many studies have shown that white rot fungi are promising microorganisms in the treatment of dye-containing wastewater, among which Phanerochaete chrysosporium is the most studied and discussed. Phanerochaete chrysosporium has a broad-spectrum degradative ability to many organic pollutants and different types of synthetic dyes (such as azo, triphenylmethane, phthalocyanine dyes) due to its non-specific, substrate-independent enzymes (Heinfling A, et al. Biodegradation of azo and phthalocyanine dyes by Trametes versicolor and Bjerkandera adusta. Appl Microbiol ...

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Problems solved by technology

But the problem of embedding method is, because the white rot fungus continues to grow, it will gradually break through the polyethylene pellets, so that the mycelium is exposed, which affects the antibacterial effect (Leidig E, et al. Biotransformation of Poly R-478 by continuous cultures of PVAL-encapsulated Trametes versicolor under non-sterile conditions. Bioprocess Eng, 1999, 21: 5-12.)

Another article is to study the control strategy of the white rot fungus Trametesversicolor to degrade reactive dyes in a non-sterile environment by controlling low pH, nitrogen-limited medium and natural carriers, but it is mentioned in the article that it is very difficult to control low pH and nitrogen-limited medium alone. Difficult to obtain long-term antibacterial effect (Judy A, et al.Competition strategies for the decolorization of a textile-reactive dye with the white-rot fungi Trametesversicolor under non-sterile conditions. Biotechnology and Bioengineering, 2003, 82 (6): 736- 744.)

Both of the above two antibacterial techniques use the Trametes versicolor strain. At present, there is no growth liquid medium for Phanerochaete chrysosporium that can effectively inhibit the growth of bacteria at home and abroad.