Bacteria growing inhibiting culture medium of white rot fungus degrading active dye in non-sterilized environment
A growth medium and white-rot fungus technology, applied in the field of applied microorganisms, can solve the problems of affecting the antibacterial effect and difficult to obtain the antibacterial effect, and achieve the effects of reducing operating costs, convenient use, and high-efficiency decolorization effect
Examples
Embodiment 1
[0029]The liquid culture medium that does not add vitamin B1 will be prepared and divided in 6 250ml conical flasks, every bottle contains 100ml liquid culture medium (this culture medium C / N=56 / 8.7mM, promptly ammonium tartrate concentration is 0.8g / L; In addition, the concentration of potassium dihydrogen phosphate is 2.0g / L, FeSO 4 .7H 2 O is 3.5mg / L). Put the Erlenmeyer flask containing the liquid medium into a sterilizer and sterilize it at 113°C for 30min. After the sterilization, filter and add 1ml of 100mg / L vitamin B1 solution to 100ml liquid medium with a syringe and a needle filter (membrane sterilized) to keep the final concentration of vitamin B1 in the liquid medium at 1mg / L. Then, the same amount of Phanerochaete chrysosporium BKM-F-1767 spores growing on the PDA plate (200g / L potato juice, 20g / L glucose and 20g / L agar) at 37°C was aseptically inserted into the liquid medium , the inoculum size was 1×10 5 spores / ml. Then the Erlenmeyer flask was placed in ...
Embodiment 2
[0031] Part of the composition of the liquid medium was changed, and the experiment of decolorizing reactive brilliant red K-2BP in a non-sterile environment was carried out. The concentration of ammonium tartrate in the liquid medium is 0.2g / L, the concentration of potassium dihydrogen phosphate is 1.0g / L, FeSO 4 .7H 2 O is 2.0mg / L; All the other composition contents are with embodiment one. The specific process of cultivating Phanerochaete chrysosporium and decolorizing reactive brilliant red K-2BP is the same as in Example 1. The results show that the decolorization effect of reactive brilliant red K-2BP is still good in a non-sterilized environment, and the decolorization rate is above 82% after adding reactive brilliant red K-2BP for 2 days; through microscopic examination of the liquid medium, only a small amount of Yeast, no bacteria were found.
Embodiment 3
[0033] Still changing some components of the liquid medium, the non-sterile environment decolorization activity brilliant red K-2BP experiment was carried out. The concentration of ammonium tartrate in the liquid medium is 0.4g / L, the concentration of potassium dihydrogen phosphate is 1.5g / L, FeSO 4 .7H 2 O is 3.0mg / L; All the other composition contents are with embodiment one. The specific process of cultivating Phanerochaete chrysosporium and decolorizing reactive brilliant red K-2BP is the same as in Example 1. The results show that the decolorization effect of reactive brilliant red K-2BP is still good in a non-sterile environment, and the decolorization rate is above 85% after adding reactive brilliant red K-2BP for 2 days; as a result of microscopic examination of the liquid medium, only a small amount of yeast was found bacteria, no bacteria were found.
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Abstract
Description
Claims
Application Information
- IPC
- C12N1/14
- Inventors
- 文湘华; 高大文