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Biology method for synthesizing beta alanine

A technology of biosynthesis and alanine, applied in the field of biosynthesis of β-alanine, can solve the problems of high cost and environmental pollution, and achieve the effect of low cost, high product purity and simple steps

Inactive Publication Date: 2005-06-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of biosynthesis method of β-alanine, to overcome the deficiency that the existing chemical synthesis method of β-alanine is easy to pollute the environment and has high cost

Method used

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  • Biology method for synthesizing beta alanine
  • Biology method for synthesizing beta alanine
  • Biology method for synthesizing beta alanine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Biosynthesis of β-alanine

[0041] Seed medium: corn steep liquor 1.1 g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.1g, sodium chloride 0.145g, beef extract 0.5g, water 100mL, sterilized at 121°C for 20min, acrylic acid 0.8g, sodium hydroxide adjusted to pH 7.0;

[0042] Fermentation medium: corn steep liquor 1.1g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.1g, sodium chloride 0.145g, water 100mL, sterilized at 121°C for 20min, acrylic acid 0.8g, sodium hydroxide adjusted to pH 7.0;

[0043] Conversion solution: acrylic acid 15g, MgSO 4 .7H 2 O 0.11g, ammonia water to adjust pH to 7.5, and 0.2M phosphate buffer to make up to 500mL.

[0044] S. luteus slant strain was inserted into the seed medium, 200r / min, 30 ℃ shaking for 24h. 20% of the inoculum was transferred to the fermentation medium, and cultured at 30° C., 200 r / min on a shaker for 18 hours to obtain a fermentation broth. The fermentation broth is added to the transformation solution at a rate of 50%. Th...

Embodiment 2

[0045] Example 2: Biosynthesis of β-alanine

[0046] Seed medium: corn steep liquor 0.5g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, sodium chloride 0.10g, beef extract 0.8g, water 100mL, sterilized at 121°C for 20min, acrylic acid 0.08g, hydrochloric acid to adjust pH to 6.5;

[0047] Fermentation medium: corn steep liquor 0.5g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, sodium chloride 0.10g, water 100mL, sterilized at 121°C for 20min, acrylic acid 0.08g, adjusted to pH 6.5 with hydrochloric acid; transformation solution: acrylic acid 10g, MgSO 4 .7H 2 O 0.10g, ammonia water to adjust pH to 4.8, and 0.2M acetate buffer to make up to 500mL.

[0048] S. luteus slant strains were inserted into the seed medium, 100 r / min, and cultured on a shaker at 25°C for 20 h. 15% of the inoculum was transferred to the fermentation medium, and cultured at 25°C with a shaker at 100 r / min for 16 hours. The fermentation broth was added to the transformation solution at a ratio of 40%, the ...

Embodiment 3

[0049] Example 3: Biosynthesis of β-alanine

[0050] Seed medium: corn steep liquor 1.8g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, sodium chloride 0.45g, beef extract 6.5g, water 100mL, sterilized at 121°C for 20min, acrylic acid 7.5g, hydrochloric acid to adjust pH to 5.5;

[0051] Fermentation medium: corn steep liquor 1.8g, MgSO 4 .7H 2 O 0.02g, KH 2 PO 4 0.05g, sodium chloride 0.45g, water 100mL, sterilized at 121°C for 20min, acrylic acid 7.5g, and hydrochloric acid adjusted to pH 5.5;

[0052] Conversion solution: acrylic acid 200g, MgSO 4 .7H 2 O 0.12g, ammonia water to adjust pH6.0, 0.1M citric acid buffer solution to 500mL.

[0053] Escherichia coli slant strains were inserted into the seed medium, and incubated at 300 r / min at 55 °C for 18 h on a shaker. 15% of the inoculum was transferred to the fermentation medium, and cultured at 55° C., 300 r / min on a shaker for 16 hours. The fermentation broth was added to the transformation solution at a rate of 50%...

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Abstract

A biologic synthesis method for beta-alanine includes providing the microbes able to generate aminating enzyme, adding them to the seed culture medium containing acrylic acid and the fermenting culture medium containing acrylic acid to culture relative aminating enzyme, adding them to the aqueous solution of ammonia containing acrylic acid, synthesizing beta-alanine under the action of enzyme, removing ammonia, and purifying. Its advantages are high efficiency, no pollution and high purity.

Description

(1) Technical field [0001] The present invention relates to a biosynthesis method of β-alanine, in particular to a method for synthesizing β-alanine under the action of microbial enzymes. (2) Background technology [0002] Beta-alanine, also known as beta-aminopropionic acid. Discovered in 1972 by Ross and Monroe in the degradation products of uracil. It is a non-protein amino acid and the only beta amino acid in nature. The main physiological activity of β-alanine is to synthesize pantothenic acid and coenzyme A. Modern medical research has found that in the mammalian nervous system, it can act as a neurotransmitter in the brain; it can activate ion channels; it can treat liver damage caused by tissue hypoxia. [0003] In the field of fine chemicals, β-alanine is used to synthesize polymerized β-alanine, electroplating buffers, dyes, etc. Polymeric β-alanine is used as a flocculant in the water treatment industry; in the pharmaceutical industry, β-alanine can be used as...

Claims

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Application Information

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IPC IPC(8): C12P13/06
Inventor 裘娟萍沈寅初姚燕来韩建娜黄海婵汪琨
Owner ZHEJIANG UNIV OF TECH
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