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Method for producing hydroxy alkanoic acid polymer

A technology of hydroxyalkanoic acid and production method, applied in biochemical equipment and methods, microorganisms, fermentation and other directions, can solve the problems of increasing production cost, affecting the scope of application, and high price

Inactive Publication Date: 2005-08-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, genetically recombinant bacteria are generally not used as production strains in the production process of food and medical products to avoid biosafety problems
(4) In the research on P(HBHH), the main raw materials used were olive oil and oleic acid, soybean oil and lauric acid, sodium gluconate and lauric acid, etc., and the downstream extraction and processing processes were not reported
Generally speaking, using oil as a carbon source to ferment and produce PHA will increase the difficulty of product extraction and often affect the quality of PHA
However, using sodium gluconate as a fermentation raw material will increase production costs and affect its application range due to its high price.

Method used

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  • Method for producing hydroxy alkanoic acid polymer
  • Method for producing hydroxy alkanoic acid polymer
  • Method for producing hydroxy alkanoic acid polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1, use glucose as the fermentative production of carbon source synthetic P (HBHH)

[0071] (1) Inclined plane and shake flask seed culture

[0072] Inoculate the preserved S. fredii strain on the slant medium, activate it twice in an incubator at 30°C, transfer it to a shake flask containing a shake flask seed medium, and activate it twice again, and wait for the next stage of shaking Flask cultured for 16 hours, when the bacterial cell concentration reached 1.79g stem cells / L (OD 600 When the value is 3.5), the cell growth is still in the logarithmic growth phase, which is used as the seed liquid of the seed tank for subsequent use.

[0073] (2) Seed tank seed culture

[0074] Use a 10L self-control tank as the seed tank, use fermented seed medium, the filling coefficient is 0.7, insert the seed solution, the inoculation amount is 8%, cultivate at 30°C, adjust and control the pH to 7.0-7.2 through 6mol NaOH solution, and maintain dissolved oxygen Over 20%...

example 2

[0082] Example 2, using starch hydrolyzate as the fermentative production of carbon source synthesis P (HBHH)

[0083] (1) Inclined plane and shake flask seed culture

[0084] Inoculate the preserved S. fredii strain on the slant medium, activate it twice in an incubator at 28°C, transfer it to a shake flask containing a shake flask seed medium, and activate it twice again, and wait for the next stage of shaking Flask cultured for 18h, when the bacterial cell concentration reached 2.81g stem cells / L (OD 600 When the value is 5.5), the cell growth is still in the logarithmic growth phase, and it is used as the seed liquid of the seed tank for subsequent use.

[0085] (2) Seed tank seed culture

[0086] Use a 10L self-control tank as the seed tank, use fermented seed medium, the filling coefficient is 0.7, insert the seed liquid, the inoculation amount is 9%, cultivate at 30°C, adjust and control the pH to 7.0-7.2 with 6mol NaOH solution, and maintain dissolved oxygen Over 20% ...

Embodiment 3

[0093] Embodiment 3, use waste molasses as the fermentative production of carbon source synthesis P (HBHH)

[0094] (1) Incline and shake flask culture

[0095] Inoculate the preserved S. fredii strain on the slant medium, activate it twice in an incubator at 32°C, transfer it to a shake flask containing a shake flask seed medium, and activate it twice again, and wait for the next stage of shaking Flask cultured for 18 hours, when the bacterial cell concentration reached 2.6g stem cells / L (OD 600 When the value is 5.2), the cell growth is still in the logarithmic growth phase, which is used as the seed liquid of the seed tank for subsequent use.

[0096] (2) Seed tank seed culture

[0097] Use a 10L self-control tank as the seed tank, use fermented seed medium, the filling coefficient is 0.7, insert the seed liquid, the inoculum amount is 10%, cultivate at 30°C, adjust and control the pH to 7.0-7.2 through 6mol NaOH solution, and maintain dissolved oxygen Over 20% saturatio...

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Abstract

The present invention discloses the production process of hydroxy alkanoic acid polymer. The hydroxy alkanoic acid polymer is prepared with Sinorhizobium fredii as production strain, carbohydrate as carbon source and ammonium salt as nitrogen source, and through high density fermentation and metabolism regulation with caprate in optimal culture medium and under optimal culture condition to obtain 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer. Corresponding fermentation management method, separation and purification method and quality detection method are established. The present invention can obtain 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer product up to 10.7-16.4 g / L, of molecular weight of 127000-137000 Da, and endotoxin content 4-6 EU / g, and the novel nanometer level material may be used in biomedicine and medical tissue engineering.

Description

technical field [0001] The present invention relates to a kind of macromolecular compound obtained by the reaction of forming carboxylate bond on the main chain of macromolecule, specifically belongs to a production method of hydroxyalkanoic acid polymer (hereinafter referred to as PHA), more specifically 3-hydroxyalkanoic acid polymer Butyric acid and 3-hydroxycaproic acid polymer [P(3-HB-co-3-HH), hereinafter referred to as P(HBHH)] production method. Background technique [0002] PHA is a kind of high molecular polymer with simple structure biosynthesized, and it is the main carbon source and energy storage material of prokaryotes. Under the condition of unbalanced metabolism, prokaryotes will use the remaining nutrients to synthesize PHA and distribute it discretely in the cells in the form of particles, and its content can reach up to 90% of the dry weight of the cells. Decomposition and utilization. It is precisely because of this biological characteristic that endow...

Claims

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Application Information

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IPC IPC(8): C08G63/06C12N5/02C12P7/00
Inventor 赵良启王海宾冯涛肖婧凡
Owner SHANXI UNIV
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