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Method for preparing porous collagen composite nano hydroox apatite artificial bone

A nano-hydroxyapatite and hydroxyapatite technology, which is applied in the fields of bioengineering and material science, can solve the problems of insufficient mechanical strength, low porosity, and high brittleness, and achieve good biocompatibility, high porosity, and Good effect of pore traffic

Inactive Publication Date: 2006-05-24
NANJING MAIDISEN MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the nanophase calcium phosphate salt collagen polymer bone composite material developed by Tsinghua University has a good bone repair effect, but from the perspective of its production process and products, there are still insufficient mechanical strength, high brittleness, low toughness, and porosity. Defects such as low and no interconnected hole structure (poor pore traffic)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Preparation of nano-sized hydroxyapatite

[0017] Weigh 44g CaCl 2 .2H 2 O was dissolved in 1000ml deionized water, stirred to fully dissolve, and 17 blood H was added while stirring 3 PO 4 , and then add 1mol / L NaOH solution to pH 7.5 while stirring. The precipitate was collected by suction filtration, washed 3 times with deionized water, filtered by suction, and the filter cake was dried in a drying oven at 100° C. for 48 hours. After grinding, a hydroxyapatite powder with a particle size of nanometer order was obtained.

[0018] (2) Preparation of collagen solution

[0019] Take the tendons of qualified pork legs after quarantine, carefully remove the fat and muscle tissue, wash with detergent to remove the grease on the surface of the tendons, rinse with deionized water, and grind the tendons to a particle size of 3-6 mm. Soak 25g of tendon in 1000ml of 0.1% phosphoric acid (analytical pure) solution, add 1200U (international unit) of pepsin, mix thoroughly...

Embodiment 2

[0024] (1) Preparation of nano-sized hydroxyapatite

[0025] Weigh 50g CaCl 2 .2H 2 O was dissolved in 1000ml deionized water, stirred to make it fully dissolved, and 26g of diammonium hydrogen phosphate ((NH 4 )2 HPO 4 ), then add 1mol / L NaOH solution to pH 8.0 while stirring. The precipitate was collected by suction filtration, washed three times with deionized water, filtered by suction, and the filter cake was dried in a drying oven at 98° C. for 48 hours. After grinding, a hydroxyapatite powder with a particle size of nanometer order was obtained.

[0026] (2) Preparation of collagen solution

[0027] Take the tendon of the qualified beef leg after quarantine, carefully remove the fat and muscle tissue, wash with detergent to remove the grease on the surface of the tendon, rinse with deionized water, and crush the tendon to a particle size of 3-6 mm. Soak 25g of tendon in 1000ml of 0.1% phosphoric acid (analytical pure) solution, add 1000U (international unit) of pep...

Embodiment 3

[0032] (1) Preparation of nano-sized hydroxyapatite

[0033] Weigh 189g calcium nitrate (Ca(NO 3 ) 2 4H 2 O) be dissolved in 1000ml deionized water, stir to make it fully dissolved, add 68g disodium hydrogen phosphate (Na 2 HPO 4 ), then add 1mol / L NaOH solution to pH 7.4 while stirring. The precipitate was collected by suction filtration, washed three times with deionized water, filtered by suction, and the filter cake was dried in a drying oven at 95° C. for 48 hours. After grinding, a hydroxyapatite powder with a particle size of nanometer order was obtained.

[0034] (2) Preparation of collagen solution

[0035] Take the qualified horse leg tendon after quarantine, carefully remove the fat and muscle tissue, wash with detergent to remove the grease on the surface of the tendon, rinse with deionized water, crush the tendon to a particle size of 3-6 mm, and crush the tendon Soak 25g in 1000ml of 0.1% phosphoric acid (analytically pure) solution, add 1500U (internationa...

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PUM

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Abstract

The preparation method of porous collagen composite nano hydroxyapatite artificial bone includes the following steps: firstly preparing nano hydroxyapatite, then preparing collagen solution, adding collagen solution into the hydroxyapatite powder body, finally mixing them and making them into pasty material, freeze-drying so as to obtain the invented porous collagen composite nano hydroxyapatite artificial bone.

Description

technical field [0001] The invention relates to a method for preparing a porous collagen composite nano-hydroxyapatite artificial bone, which is used for repairing bone defects and fractures, and belongs to the technical fields of bioengineering and material science. Background technique [0002] Bone transplantation is the most needed graft after blood transfusion, and bone defect caused by accident or disease is a common clinical case. At present, the commonly used repair materials include autologous bone, allogeneic bone and artificial synthetic materials. These materials are insufficient to varying degrees and cannot meet the clinical needs. For example, although autologous bone transplantation has no immune rejection and has a good repair effect, the material obtained is very limited and causes bone defects in the donor site; allogeneic bone transplantation is likely to cause immune rejection, poor repair effect, and the risk of infectious viral diseases , and the cost...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61B17/56A61F2/28A61L27/00A61L27/24
Inventor 胡庆柳张居适李志龙沈建成
Owner NANJING MAIDISEN MEDICAL TECH
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