Method for preparing plant source protein three-dimensional steut with improved property

[0015] Example one

[0016] Mix oleic acid and plant-derived gliadin at a mass ratio of 0.1:1, and then add mannitol at a ratio of 1:9 to the mass ratio of porogen to plant-derived gliadin, mix well, and press molding. , And then put the prepared samples in 95% relative humidity, 40 ℃ conditions for 21 days. The plant-derived gliadin is a gliadin derived from corn. After the stent was formed, the stent was placed in a 55°C water bath to dissolve the porogen in the water and removed from the stent. The water was replaced every two hours, and the leaching time was 72 hours. The plant-derived protein three-dimensional scaffold can be prepared by vacuum freeze-drying for 12 hours. The prepared samples are tested by Zwick material universal testing machine for mechanical properties and the porosity test by the gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 83.3 MPa and 877 MPa, respectively; the bending strength and modulus reached 19.7 MPa and 529 MPa, respectively; the tensile strength and modulus were 5.2 MPa and 468 MPa, respectively. The porosity of the scaffold reaches 28.0%.

[0017] Example two

[0018] Mix stearic acid and plant-derived protein at a mass ratio of 0.1:1, and then add sorbitol in a ratio of 3:7 mass ratio of porogen to plant-derived gliadin, mix well, and press molding. Then, the prepared sample was placed in a relative humidity of 97%, and molded at 37°C for 35 days. The plant-derived gliadin is a gliadin derived from rye. After the stent is formed, the stent is placed in a 40°C water bath to dissolve the porogen in the water and removed from the stent. The water is replaced every two hours, and the leaching time is 60 hours. The plant-derived protein three-dimensional scaffold can be prepared by vacuum freeze-drying for 12 hours. The prepared samples are tested by Zwick material universal testing machine for mechanical properties and the porosity test by the gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 15.3 MPa and 339 MPa, respectively; the bending strength and modulus reached 6.2 MPa and 308 MPa, respectively; the tensile strength and modulus were 1.6 MPa and 378 MPa, respectively. The porosity of the scaffold reaches 53.5%.

[0019] Example three

[0020] Mix linoleic acid and plant-derived gliadin at a mass ratio of 0.1:1, and then add lactose at a mass ratio of 7:3 in the ratio of porogen to plant-derived gliadin, mix well, and press molding. . Then the prepared samples were placed in a relative humidity of 100%, 45°C for 14 days. The plant-derived gliadin is a gliadin derived from rice. After the stent is formed, the stent is placed in a 60°C water bath to dissolve the porogen in the water and removed from the stent. The water is replaced every two hours, and the leaching time is 48 hours. The plant-derived protein three-dimensional scaffold can be prepared by vacuum freeze-drying for 12 hours. The prepared samples are tested by Zwick material universal testing machine for mechanical properties and the porosity test by the gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 3.9 MPa and 27 MPa, respectively; the bending strength and modulus reached 1.3 MPa and 19 MPa, respectively; the tensile strength and modulus were 0.5 MPa and 9 MPa, respectively. The porosity of the scaffold reaches 82.5%.