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Method for preparing plant source protein three-dimensional steut with improved property

A plant-derived, three-dimensional scaffold technology, applied in the field of biomedical engineering, can solve the problems of high material brittleness, poor mechanical properties, and unsuitable load-bearing parts, etc., and achieve the effect of wide source of raw materials, improved mechanical properties, and low process requirements

Inactive Publication Date: 2006-05-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method can obtain a scaffold with certain mechanical strength and pore characteristics, its mechanical properties are poor, the compressive modulus is only tens of MPa, and the material is too brittle to obtain tensile and bending properties.
Therefore, the application of the plant-derived protein three-dimensional scaffold prepared by the above method is limited in tissue engineering, and it is not suitable for load-bearing parts, especially the needs of bone tissue engineering.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Mix oleic acid and plant-derived gliadin at a mass ratio of 0.1:1, then add mannitol in a mass ratio of pore-forming agent to plant-derived gliadin at a ratio of 1:9, mix well, and mold , and then the prepared samples were molded for 21 days at a relative humidity of 95% and 40°C. The plant-derived gliadin is a kind of gliadin derived from corn. After the scaffold was formed, the scaffold was placed in a 55°C water bath to dissolve the porogen in the water and remove it from the scaffold. The water was changed every two hours, and the leaching time was 72 hours. After 12 hours of vacuum freeze-drying, the plant-derived protein three-dimensional scaffold can be prepared. The prepared samples were tested by Zwick material universal testing machine for mechanical property test and porosity test by gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 83.3MPa and 877MPa; the bending strength and modulus re...

Embodiment 2

[0018] Mix stearic acid and plant-derived protein at a mass ratio of 0.1:1, then add sorbitol in a mass ratio of pore-forming agent to plant-derived gliadin of 3:7, mix well, and then mold it into shape. Then the prepared samples were molded for 35 days at a relative humidity of 97% and 37°C. The plant-derived gliadin is a gliadin derived from rye. After the scaffold was formed, the scaffold was placed in a 40°C water bath to dissolve the porogen in the water and remove it from the scaffold. The water was changed every two hours, and the leaching time was 60 hours. After 12 hours of vacuum freeze-drying, the plant-derived protein three-dimensional scaffold can be prepared. The prepared samples were tested by Zwick material universal testing machine for mechanical property test and porosity test by gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 15.3MPa and 339MPa; the bending strength and modulus reache...

Embodiment 3

[0020] Mix linoleic acid and plant-derived gliadin at a mass ratio of 0.1:1, then add lactose in a ratio of 7:3 to the mass ratio of pore-forming agent and plant-derived gliadin, mix well, and mold . Then the prepared samples were molded for 14 days at a relative humidity of 100% and 45°C. The plant-derived gliadin is a kind of gliadin derived from rice. After the scaffold was formed, the scaffold was placed in a 60°C water bath to dissolve the porogen in the water and remove it from the scaffold. The water was changed every two hours, and the leaching time was 48 hours. After 12 hours of vacuum freeze-drying, the plant-derived protein three-dimensional scaffold can be prepared. The prepared samples were tested by Zwick material universal testing machine for mechanical property test and porosity test by gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 3.9MPa and 27MPa respectively; the bending strength ...

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Abstract

The present invention relates to a preparation method for improving plant-source protein three-dimensional scaffold performance. Said preparation method includes the following steps: firstly, mixing fatty acid and plant-source alcohol-soluble protein according to a certain ratio, then adding pore-forming agent, die-pressing and forming, then strengthening and forming, adopting water bath and filtering to remove pore-forming agent, freeze-drying so as to obtain the plant-source protein three-dimensional scaffold with high mechanical strength and porosity, in which the mass ratio of described fatty acid and plant-source alcohol-soluble protein is 0.1:1-0.6:1, and the mass ratio of pore-forming agent and plant-source alcohol-soluble protein is 1:9-7:3.

Description

technical field [0001] The invention relates to a method in the technical field of biomedical engineering, in particular to a preparation method for improving the performance of a plant-derived protein three-dimensional scaffold. Background technique [0002] Scaffold materials for tissue engineering are one of the focuses of tissue engineering research in the field of biomedicine. Biomaterials are three-dimensional scaffolds on which seed cells live and attach before forming tissues. It can fix cells in a certain position, provide a place for physiological activities such as growth, reproduction, metabolism, and extracellular matrix secretion, and guide the basic shape of regenerated tissues. . Scaffold materials for tissue engineering should have the following characteristics: ① Good biocompatibility and tissue compatibility: conducive to cell adhesion and proliferation, no cytotoxicity, no immunogenicity, and no inflammatory response; ② Biodegradability: It can be compl...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/56A61L31/10
Inventor 王瑾晔龚生举
Owner SHANGHAI JIAO TONG UNIV
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