Method for preparing plant source protein three-dimensional steut with improved property

A plant-derived, three-dimensional scaffold technology, applied in the field of biomedical engineering, can solve the problems of high material brittleness, poor mechanical properties, and unsuitable load-bearing parts, etc., and achieve the effect of wide source of raw materials, improved mechanical properties, and low process requirements

Inactive Publication Date: 2006-05-24
SHANGHAI JIAO TONG UNIV
1 Cites 2 Cited by

AI-Extracted Technical Summary

Problems solved by technology

Although the above method can obtain a scaffold with certain mechanical strength and pore characteristics, its mechanical properties are poor, the compressive modulus is only tens of MPa, and the material is too brittle to obtain tensile and bending p...
View more

Abstract

The present invention relates to a preparation method for improving plant-source protein three-dimensional scaffold performance. Said preparation method includes the following steps: firstly, mixing fatty acid and plant-source alcohol-soluble protein according to a certain ratio, then adding pore-forming agent, die-pressing and forming, then strengthening and forming, adopting water bath and filtering to remove pore-forming agent, freeze-drying so as to obtain the plant-source protein three-dimensional scaffold with high mechanical strength and porosity, in which the mass ratio of described fatty acid and plant-source alcohol-soluble protein is 0.1:1-0.6:1, and the mass ratio of pore-forming agent and plant-source alcohol-soluble protein is 1:9-7:3.

Application Domain

CoatingsProsthesis

Technology Topic

ChemistryFreeze dry +10

Examples

  • Experimental program(9)

Example Embodiment

[0015] Example one
[0016] Mix oleic acid and plant-derived gliadin at a mass ratio of 0.1:1, and then add mannitol at a ratio of 1:9 to the mass ratio of porogen to plant-derived gliadin, mix well, and press molding. , And then put the prepared samples in 95% relative humidity, 40 ℃ conditions for 21 days. The plant-derived gliadin is a gliadin derived from corn. After the stent was formed, the stent was placed in a 55°C water bath to dissolve the porogen in the water and removed from the stent. The water was replaced every two hours, and the leaching time was 72 hours. The plant-derived protein three-dimensional scaffold can be prepared by vacuum freeze-drying for 12 hours. The prepared samples are tested by Zwick material universal testing machine for mechanical properties and the porosity test by the gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 83.3 MPa and 877 MPa, respectively; the bending strength and modulus reached 19.7 MPa and 529 MPa, respectively; the tensile strength and modulus were 5.2 MPa and 468 MPa, respectively. The porosity of the scaffold reaches 28.0%.

Example Embodiment

[0017] Example two
[0018] Mix stearic acid and plant-derived protein at a mass ratio of 0.1:1, and then add sorbitol in a ratio of 3:7 mass ratio of porogen to plant-derived gliadin, mix well, and press molding. Then, the prepared sample was placed in a relative humidity of 97%, and molded at 37°C for 35 days. The plant-derived gliadin is a gliadin derived from rye. After the stent is formed, the stent is placed in a 40°C water bath to dissolve the porogen in the water and removed from the stent. The water is replaced every two hours, and the leaching time is 60 hours. The plant-derived protein three-dimensional scaffold can be prepared by vacuum freeze-drying for 12 hours. The prepared samples are tested by Zwick material universal testing machine for mechanical properties and the porosity test by the gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 15.3 MPa and 339 MPa, respectively; the bending strength and modulus reached 6.2 MPa and 308 MPa, respectively; the tensile strength and modulus were 1.6 MPa and 378 MPa, respectively. The porosity of the scaffold reaches 53.5%.

Example Embodiment

[0019] Example three
[0020] Mix linoleic acid and plant-derived gliadin at a mass ratio of 0.1:1, and then add lactose at a mass ratio of 7:3 in the ratio of porogen to plant-derived gliadin, mix well, and press molding. . Then the prepared samples were placed in a relative humidity of 100%, 45°C for 14 days. The plant-derived gliadin is a gliadin derived from rice. After the stent is formed, the stent is placed in a 60°C water bath to dissolve the porogen in the water and removed from the stent. The water is replaced every two hours, and the leaching time is 48 hours. The plant-derived protein three-dimensional scaffold can be prepared by vacuum freeze-drying for 12 hours. The prepared samples are tested by Zwick material universal testing machine for mechanical properties and the porosity test by the gas exhaust method. The compressive strength and modulus of the plant-derived protein three-dimensional scaffold reached 3.9 MPa and 27 MPa, respectively; the bending strength and modulus reached 1.3 MPa and 19 MPa, respectively; the tensile strength and modulus were 0.5 MPa and 9 MPa, respectively. The porosity of the scaffold reaches 82.5%.

PUM

PropertyMeasurementUnit
Modulus83.3 ~ 877.0mPa
Modulus19.7 ~ 529.0mPa
Modulus5.2 ~ 468.0mPa

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

Similar technology patents

Alkliproof corrosion resistant ceramic packing and producing technique

InactiveCN1519050AExcellent alkali corrosion resistanceExtensive sources of raw materials
Owner:FUJIAN JUNGIE NEW MATERIAL TECH CO LTD

Method for producing white microcrystalline wax by one-step hydrogenation

ActiveCN1769395AReduce equipment investment and operating costsExtensive sources of raw materials
Owner:CHINA PETROLEUM & CHEM CORP +1

Method for producing edible fungus with high yield by carrying out enzymolysis on crop straws with enzymatic microorganisms

InactiveCN102550282AExtensive sources of raw materialsSimplify the cultivation process of edible fungi
Owner:武汉楚天绿色科技开发有限公司

Preparation method of glutamine dipeptide

Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Classification and recommendation of technical efficacy words

  • Extensive sources of raw materials
  • low price

Two-stage gasification method and gasification device for fuels with wide size distribution

ActiveCN102703131AExtensive sources of raw materialsWide particle size distribution
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Anode material for lithium-ion battery and preparation method of anode material

InactiveCN104577081Alow priceSufficient raw materials
Owner:SOUTH CHINA UNIV OF TECH

Vehicle maintenance prompting system

InactiveCN101301872Alow price
Owner:徐立
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products