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Separation and selection tool based on Au nano channel array and preparation method thereof

A technology for separation and screening of gold nanochannels, applied in the field of biological nano-screening tools, separation and screening tools based on gold nanochannel arrays and its preparation, can solve the problem of difficult separation and detection, low stability of double-layer membrane structure , easy to break and other problems, to achieve the effect of simple and easy cleaning and regeneration process, shortened separation operation cost, and short residence time

Inactive Publication Date: 2006-07-12
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low stability of the traditional double-layer membrane structure, it is easy to break, and it is difficult to play the role of separation and detection in many actual detection technology environments.

Method used

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  • Separation and selection tool based on Au nano channel array and preparation method thereof
  • Separation and selection tool based on Au nano channel array and preparation method thereof
  • Separation and selection tool based on Au nano channel array and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, preparation of template material 2' based on gold nanochannel array:

[0050] The template material 2' (made of polyalkoxy block copolymer, or maltose and its derivatives, or tartaric acid and its derivatives, or methylol propionic acid, or glycerol, or pentaerythritol, or glucose and its polymers, Wherein one or several template materials 2' made; or aluminum oxide membrane, or glass membrane, or polycarbonate filter membrane, or aluminum oxide membrane, or silicon oxide membrane, or plastic membrane, or glass fiber membrane) is immersed in In absolute ethanol, ultrasonically oscillate for 10 minutes, then rinse with absolute ethanol 2-3 times. Wash the template material 2′ in SnCl 2 Soak in the solution for 60min. After fully rinsing, put in AgNO 3 Soak in the solution for 10min. Rinse the activated template material 2′ with water 5 times, and place in HAuCl with glucose 4 In the gold deposition solution, keep the temperature at 5°C and deposit on a...

Embodiment 2

[0051] Embodiment 2, template material 2 ' (membrane) based on gold nanochannel array to the separation performance of medicine:

[0052] Porous alumina with a pore diameter of 100 nm was used as the template material 2' (membrane), and the gold sulfite deposition solution added with formaldehyde solution was used to prepare the gold nanochannel array template material 2' (membrane) according to the process of Example 1. The prepared gold nanochannel array template material 2′ (membrane) was immersed in the cysteine ​​solution, and N was continuously introduced during the soaking process. 2 . Cysteine ​​is self-assembled on the surface of gold through sulfhydryl groups, and then the gold nanochannel array template material 2' (membrane) is rinsed with ethanol and dried naturally in the air.

[0053] use as figure 1 In the separation device shown, the gold nanochannel array template material 2' (membrane) of the present invention is placed in the middle of the flow cell and s...

Embodiment 3

[0055] Embodiment 3, the separation performance of the template material 2' (membrane) based on the gold nanochannel array to biomacromolecules:

[0056] A gold nanochannel array was prepared using a glass membrane as a template material 2' (membrane). Observed by an atomic force microscope, there are about 10 per square centimeter 8 A gold nanochannel with an inner diameter of about 55 nm and a length of 10 μm. The cysteine-modified gold nanochannel array template material 2' (membrane) was used as the separated carrier, and the effect of the membrane on the mixed solution of bovine serum albumin (BSA) and immunoglobulin (IGg) was measured when the pH value was 4.5. The separation effect of , denoted by S' and S respectively. S' is the mass of BSA and IgG per unit area in the permeation cell (mg / cm 2 ) with time t (h) change ratio), S is the percentage penetration of BSA and IgG per unit area in the permeation cell (% / cm 2 ) is the ratio of change with time t(h). After a...

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Abstract

The invention relates to a separation filtration device based on the golden nanometer channel array, which integrates a nanometer pipe array with uniform diameter in the solid support as the mold material of inorganic golden nanometer channel array. The preparation of said golden nanometer channel array of mold material comprises six steps: washing mold material; hypersensitizing, activating, the aggradation of nanometer gold; washing channel; and the decoration of golden nanometer channel array. Since used support is easy to be attained with simple synthesizing method, said golden nanometer channel has better rigidity while its surface is easy to generate variable chemical reactions, and it can induce selective factor easily to realize the separation and detection of biological active material, natural medical macromolecule and environment pollution material. And it is nondestructive inspection with high separation efficiency to apply the actual inspection separation environment. Said preparation method of separation filtration device has better generality and advanced property.

Description

technical field [0001] The invention relates to the improvement of biological nano-screening tool technology, specifically a separation and screening tool based on gold nanochannel array and its preparation method, which belongs to the science and technology of separation, purification and analysis of drugs, biological macromolecules, organic molecules and ions field. Background technique [0002] In today's separation and analysis science and technology, how to effectively separate and detect biologically relevant components under the premise of ensuring biological activity is a hot issue that is generally concerned by those skilled in the art. For example, there is a single channel based on the formation of α-hemolysin (α-HL) across the phospholipid bilayer cell membrane, which has shown important application value in technical research in this field. The single-channel detection technology and its development provide a measurement basis for the establishment of nano-chan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D71/02B01D69/10
Inventor 殷月芬黎先春王小如殷月红宋秘钊
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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