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Preparation of acrylon nano fiber membrane carrier materials and use thereof

A technology of nanofiber membrane and carrier material, which is applied in the directions of immobilization on/in organic carriers, fiber processing, fiber chemical characteristics, etc. Small surface area etc.

Inactive Publication Date: 2006-10-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The specific surface area of ​​existing materials is small, it is difficult to greatly increase the number of immobilized biomolecules and enzymes, which is not conducive to reducing production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Example 1: the preparation of the acrylic nanofiber membrane carrier material containing reactive groups: 6 grams of acrylonitrile and maleic acid copolymers with a weight-average molecular weight of 100,000 are dissolved in dimethylformamide and become a transparent solution to obtain The spinning solution is added to the micro-injector, connected to the spinneret, and the spinneret is connected to a high-voltage power supply. The distance between the spinneret and the acceptor is about 15 cm, and the flow rate of the spinning solution is 5ml / h , with a voltage of 10 kilovolts, and electrospinning to obtain acrylonitrile-maleic acid copolymer nanofiber membrane carrier material, and the fiber diameter is 100-500nm.

[0023] Immobilization of biomolecules: 1 gram of acrylonitrile and maleic acid copolymer nanofiber membrane carrier material is put into 100 milliliters of phosphate buffer solution (pH=7) of 30 mg / ml collagen, and 1 gram of carbodiimide is added After rea...

example 2

[0024] Example 2: Preparation of acrylic nanofiber membrane carrier material containing reactive groups: 5 grams of acrylonitrile and acrylic acid copolymer with a weight average molecular weight of 150,000 are dissolved in dimethyl sulfoxide to become a transparent solution to obtain a spinning solution , into the micro-injector, connected to the spinneret, the spinneret is connected to a high-voltage power supply, the distance between the spinneret and the acceptor is about 20 cm, the flow rate of the spinning solution is 10ml / h, and the voltage is Electrospinning at 12 kV to obtain acrylonitrile and acrylic acid copolymer nanofiber membrane carrier material with a fiber diameter of 150-600nm.

[0025] Immobilization of biomolecules: put 1 gram of acrylonitrile and acrylic acid copolymer nanofiber membrane carrier material into 100 milliliters of phosphate buffer solution (pH=7) of 35 mg / ml gelatin, add 1 gram of carbodiimide, and react for 24 After one hour, the acrylonitri...

example 3

[0026] Example 3: Preparation of acrylic nanofiber membrane carrier material containing reactive groups: 8 grams of acrylonitrile and maleic acid copolymers with a weight average molecular weight of 80,000 were dissolved in dimethyl sulfoxide to become a transparent solution, and spinning was obtained. The silk solution is added to the micro-injector, connected to the spinneret, and the spinneret is connected to a high-voltage power supply. The distance between the spinneret and the acceptor is about 16 cm, and the flow rate of the spinning solution is 6ml / h. The voltage is 12 kilovolts, and electrospinning is carried out to obtain acrylonitrile and maleic acid copolymer nanofiber membrane carrier material, and the fiber diameter is 120-500nm.

[0027] Immobilization of biomolecules: 1 gram of acrylonitrile and maleic acid copolymer nanofiber membrane carrier material is put into 100 milliliters of phosphate buffer solution (pH=7) of 20 mg / ml heparin, and 1 gram of carbodiimide...

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Abstract

This invention relates to the preparation method for the acrylic fibers nanometer fiber film with reaction group, which includes following steps: dissolving the polyacrylonitrile copolymer with reaction group in the solvent to make the solvent become to the transparent solution, then adding the solution in the micro scale sampling device, connecting the spinning head with the high-voltage power, wherein the voltage is 5 kv-20 kv, electrostatic spinning to obtain the acrylic fibers nanometer fiber film materials with reaction group. This invention fixes the biological molecule or enzyme on the material surface by physics adsorption or chemical covalent binding connection. The material can be used in organizing project frame, the enzyme's fixing, the biological molecule's segregation.

Description

technical field [0001] The invention belongs to the preparation technology of an acrylic nanofiber membrane carrier material containing reactive groups and its method for immobilizing biomolecules and enzymes. Background technique [0002] Electrospinning technology is to spin a polymer solution or melt under an electric field, stretch it due to electrostatic action, and obtain ultrafine fibers after cooling or solvent volatilization. During the electrospinning process, ultrafine nanofiber membranes with stackable ultrafine fibers can be formed. [0003] The existing preparation methods of electrospun membranes are as follows: [0004] CN 200410019230.9; 200410019231.3 Electrospinning chitosan, poly α-hydroxy acid ester / chitosan to prepare superfine fiber membrane, this material can be used for tissue repair. [0005] CN 03131586.0 Electrospinning polymer materials such as polyester, polyamide, polyether, etc. to obtain nanofiber membranes, superimposing ordinary polymer f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F6/18D01D5/00C12N11/08C12N11/04
Inventor 徐志康叶鹏黄小军
Owner ZHEJIANG UNIV