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Method for preparing total flavones of bastardtoad flax

A technology of thymegrass and total flavonoids, applied in the field of traditional Chinese medicine preparation, can solve the problems of unclear effective parts and low content of total flavonoids, and achieve the effect of high yield and simple process

Inactive Publication Date: 2006-12-13
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: the raw materials used in the thyme grass preparation are the crude extract of thyme grass, the effective part is not clear, and the content of total flavonoids is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Take the dried thyme herbal material, crush it through a 40-mesh sieve, weigh 25g, add 500ml of 70% ethanol, heat and reflux for 2 hours, filter, add 250ml of 70% ethanol to the dregs, reflux for 1 hour, filter to remove the residue, and combine The filtrate was allowed to stand for 12 hours, centrifuged at 4000 rpm for 10 minutes, the supernatant was taken, the ethanol was recovered under reduced pressure, and dried under reduced pressure at 40°C. Dissolve the crude extract with 60ml of 95% ethanol, add 20ml of 1% basic lead acetate aqueous solution dropwise while stirring, let stand for 0.5 hours, and precipitate; suction filter the precipitate to dryness, add 60ml of 95% ethanol to suspend, and add 5 drops while stirring. % Sodium carbonate aqueous solution 10ml, let stand for 0.5 hours, precipitate, filter, add dilute hydrochloric acid to the filtrate to adjust the PH value to neutral, recover ethanol under reduced pressure, add water to 60ml, add 120ml n-butanol, extra...

example 2

[0020] Take the dried thyme herb material, crush it through a 40-mesh sieve, weigh 5g, add 100ml of 70% ethanol, heat to reflux for 2 hours, filter, add 50ml of 70% ethanol to the dregs, reflux for 1 hour, filter to remove the residue, and combine the filtrate ,Stand for 12 hours, centrifuge at 4000 rpm for 10 minutes, take the supernatant, recover ethanol under reduced pressure, add water to the remaining liquid to 50ml, place 100g on the HZ-80 macroporous adsorption resin chromatography column, after adsorption , Add distilled water to rinse until the effluent is colorless, then rinse the column with 60% ethanol, 10ml / min, until the effluent is free of flavonoid reaction. The above 60% ethanol effluents were combined, ethanol was recovered under reduced pressure, and dried under reduced pressure at 40° C. to obtain 0.235 g of brown-red powder with a yield of 4.7% (based on crude drug). Aluminum trichloride develops color, and its content is 19.7% determined by spectrophotometry....

example 3

[0022] Take the dried thyme herbal material, crush it through a 40-mesh sieve, weigh 10g, add 200ml of 70% ethanol, heat to reflux for 2 hours, filter, add 100ml of 70% ethanol to the dregs, reflux for 1 hour, filter to remove the residue, and combine the filtrate , Stand for 12 hours, centrifuge at 4000 rpm for 10 minutes, take the supernatant, recover the ethanol under reduced pressure, and dry under reduced pressure at 40°C to obtain 3.5 g of extract; add the crude thyme extract to 0.3 times column chromatography to polymerize Stir the amide powder evenly and dry at 50°C; after drying, the powder is placed on a polyamide resin chromatography column with 4 times the volume, rinsed with distilled water until the effluent is colorless, and then 30% ethanol, 50% ethanol and 70% ethanol, 10ml / min flush the column, while using thymusin I, thymusin II and thymusin III as the reference materials, thin layer chromatography to detect the effluent components; combine the fractions contai...

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PUM

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Abstract

Disclosed is a process for preparing total flavonoids of thesium Chinese turcz comprising the following steps: (1) preparing crude extract of thesium Chinese turcz, preparing flavones through macroscopic adsorption resin column method, the process can realize a content of the total flavones to 8%, 20% and 52%.

Description

[0001] The invention is based on the patent application number: 2005100249320, the name of the patent application: "the preparation method of the total flavones of Thymus grass", the patent applicant is: Shanghai Jiaotong University, the patent application date is: the patent division of April 7, 2005 Application. Technical field [0002] The invention relates to a method for preparing total flavonoids, in particular, to a method for preparing thyme total flavonoids. It belongs to the technical field of traditional Chinese medicine preparation. Background technique [0003] Thyme grass is a dry whole plant of Thesium chinense Turcz. Thyme grass is pungent, slightly bitter, and cold in nature. Return to lung, spleen, kidney channel. The whole herb is used as medicine to clear away heat, detoxify, and invigorate the kidney and astringent essence. It is mostly used to treat acute mastitis, acute tonsillitis, upper respiratory tract infection, pneumonia, pharyngitis and other inflamma...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P29/00A61K127/00
Inventor 李绍顺王峥
Owner SHANGHAI JIAO TONG UNIV
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