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Composite organic-inorganic material film as immobilized cell carrier

An inorganic composite material, immobilized cell technology, applied in the direction of being fixed on/in organic carriers, fungi, etc., can solve the problems of small specific surface, poor strength, accelerated microbial reproduction, etc. The effect of good cell affinity and increasing the advantages of industrial application

Inactive Publication Date: 2007-01-03
南开大学云南研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the above-mentioned carrier materials, polyurethane, sodium alginate, polyvinyl alcohol, etc. are commonly used embedding carriers, which have the advantages of non-toxicity to microorganisms, abundant sources, and low price, but their strength is poor and their specific surface area is small.
Alumina and its precursors such as pseudoboehmite, boehmite and mesoporous molecular sieve MCM-41 have good biocompatibility and unique pore structure, which can provide a suitable growth environment for microbial cells. After being added to the polymer substance for modification, the strength of the composite carrier is enhanced, with more pore grid structure and better permeability, so that the reproduction speed of microorganisms is accelerated, the number per unit volume is more, and the efficiency of alcohol fermentation is improved. The addition of these inorganic materials to modify polymers has not been reported

Method used

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  • Composite organic-inorganic material film as immobilized cell carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] According to the quality, take 2.50 parts of polyether, 0.02 parts of silicone oil, 0.01 parts of triethylenediamine, 0.01 parts of stannous octoate, and 0.08 parts of water. 0.40 parts, 0.40 parts of MCM-41 mesoporous molecular sieve, 1.20 parts of toluene diisocyanate was added under stirring, and after mixing, it was placed in a mold and foamed into a film to obtain a microorganism-immobilized carrier; the microorganism-immobilized carrier was added to yeast In the bacterial suspension with a concentration of 1.5-2 billion / mL, put it into the molasses medium (the added ingredients are similar to the YEPD medium, the same below) for proliferation and culture; after training for 3-6 days, the amount of carrier embedding can reach 3-5 billion cells / mL, when the number of exuded free cells reaches more than 100 million cells / mL, it is transferred to the fermentation stage.

[0017] In a columnar fermenter with a volume of 1L, add the above-mentioned immobilized carrier a...

Embodiment 2

[0019] According to the quality, take 5.00 parts of polyether, 0.05 parts of silicone oil, 0.02 parts of triethylenediamine, 0.02 parts of stannous octoate, and 0.18 parts of water. 1.00 parts of stone, 1.00 parts of MCM-41 mesoporous molecular sieve, add 2.40 parts of toluene diisocyanate under stirring, mix well and place in a mold to form a film. The microorganism immobilization carrier is added into the bacterial suspension with a yeast concentration of 1.5-2 billion / mL, and put into the molasses medium for proliferation and cultivation. After training and culturing for 3-6 days, the amount of carrier embedding can reach 3-5 billion / mL, and the number of exuded free cells can reach more than 120 million / mL, and then transfer to the fermentation stage.

[0020] In a columnar fermentation tank with a volume of 1L, immobilized carriers were added according to a filling rate of 15.00%, 200g / L of raw molasses was added, and oxygen was continuously pumped to carry out batch ferm...

Embodiment 3

[0022] According to the mass, take 4.00 parts of sodium alginate, 8.00 parts of polyvinyl alcohol, and 88.00 parts of water, stir and heat at 100-120°C until dissolved and mixed uniformly, then add 0.40 parts of boehmite and 0.40 parts of MCM-41 and mix well; After sterilization, cool to room temperature, add 20 parts of yeast suspension with a concentration of 1.5-2 billion / mL, stir evenly, and use a film coating machine to coat the film with a film thickness of 3 mm; immerse the film in 2% chlorinated Solidified in calcium solution for 4 hours; after washing with a large amount of water, put it into molasses culture medium and proliferate with oxygen. After 40-72 hours of cultivation, the amount of carrier embedding can reach 3-5 billion / mL, and the number of exuded free cells can reach More than 160 million / mL, transfer to the fermentation stage.

[0023] In a columnar fermenter with a volume of 1L, add the above-mentioned immobilized carrier according to the filling rate o...

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Abstract

The present invention is one kind of composite organic-inorganic material film as immobilized cell carrier. The composite organic-inorganic material film is prepared through adding alumina precursor, such as boehmite, pseudo boehmite, mesoporous molecular sieve MCM-41, etc to polymer material, such as foamed polyurethane, sodium alginate, PVA, etc. for modification. It is superior to traditional immobilized cell carrier material, and has unique pore canal structure for microbe cell to habitat and to propagate. It has coating and adsorbing amount up to 3-6 billion / ml and free fermenting cell up to 0.08-0.16 billion / ml, and may be used for immobilized cell, especially alcohol fermentation to shorten fermentation period and raise alcohol yield. It has the features of high permeability, high mechanical strength, high chemical stability and capacity of being reused, and is one kind of high efficiency immobilized cell carrier.

Description

technical field [0001] The invention relates to a cell immobilized carrier material, in particular to an immobilized cell organic-inorganic composite film carrier applied to the fermentation of molasses or starch raw material alcohol yeast. Background technique [0002] Immobilized cell technology is a new biotechnology developed in the 1960s. Compared with the traditional free cell fermentation method, it has high processing efficiency, good stability, and large microbial load. It has the incomparable advantages of free cell fermentation. Therefore, immobilized cell technology has become the focus of research in the past 10 years. [0003] The activity of immobilized microbial catalysts has a great relationship with the type of immobilized carrier and its preparation method. Traditional immobilized carrier materials such as polyacrylamide, calcium alginate, polyvinyl chloride, diatomaceous earth, ceramics, etc. have biochemical stability. Due to the disadvantages of poor p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C12N11/08C12N1/16
Inventor 郑春明关乃佳耿长江陶耀佳钟元俊
Owner 南开大学云南研究院
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