Magnetic sorting material and producing method and application thereof

A technology of magnetic separation and polymer materials, applied in the field of magnetic materials for the separation of biological samples, can solve the problem of low magnetic induction intensity, and achieve the effect of uniform size, regular shape and low coercive force

Inactive Publication Date: 2007-01-03
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low magnetic induction intensity generated by the ordinary permanent magnet ma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1

[0038] Preparation of magnetic sorting pellets: using splash quenching method.

[0039] Take three parts of soft iron material, and their components and weight percentages are respectively: iron 99.0% + carbon 0.04%, iron 99.5% + carbon 0.001%, and iron 99.8% + carbon 0.01%, the balance contains a very small amount of aluminum, Elements such as sodium, zinc, chromium, nickel, copper, and manganese. The three materials are melted at a high temperature of 1500°C-1800°C. The molten soft iron is sprayed at a speed of 3-50 m / s, dropped from a high altitude or dropped by a centrifugal method, using the surface tension of the liquid phase to automatically shrink into a spherical shape. Falling into the water, it suddenly condenses and solidifies into small iron balls.

Example Embodiment

[0040] Example 2

[0041] Particle size screening: The particle size of the pellets of the three materials prepared in Example 1 is mostly 0.2 to 1 mm. The particle size of the iron balls is different, and the magnetic properties are also different. In the present invention, a sample sieve is used for particle size screening: a sample sieve with a suitable mesh size is selected to screen small balls with target particle sizes of 0.01-0.1 mm, 0.1-0.2 mm and 0.2-1 mm respectively. The iron balls with a larger particle size of 0.2~1mm can be used to process a large number of samples, and are suitable for separating magnetically labeled biological samples with strong magnetic response. An iron ball with a smaller particle size of 0.01-0.1mm or 0.1-0.2mm can generate a magnetic field with a larger gradient, which is suitable for separating magnetically labeled biological samples with weaker magnetic response.

[0042]Density screening: The main component of the pellets is iron. In the ...

Example Embodiment

[0044] Example 3

[0045] Improve the magnetic permeability: the stress caused by the quenching makes the magnetic properties of the pellets unable to meet the requirements of the materials used. It is necessary to eliminate the stress through a suitable annealing process, thereby increasing the permeability of the small iron ball and reducing its coercivity. The temperature rises with the furnace to 400-900°C, the temperature is controlled to 700-1000°C, the temperature is kept for 1-5 hours, and the temperature is controlled to 400-900°C, preferably the temperature rises to 800°C with the furnace, the temperature is controlled to 900°C, and the temperature is kept for 4 hours, Control the speed to cool down to 500℃. In order to avoid oxidation of the pellets, the annealing of the pellets is carried out in a vacuum furnace or a hydrogen atmosphere. The annealed pellets have refined grains, uniform structure, low hardness, low coercivity (Hc=30~150A / m) and high maximum permeabilit...

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PUM

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Abstract

The invention relates to a kind of magnetic sorting material and its preparation method and supplication. The material includes by weight 99.0-99.8% Fe, 0-0.04% C and some impurity. The product coercive force is 30-150A/m and the maximum magnetic conductivity is between 4000 and 9000. The invention supplies a method of processing the magnetic material into uniform pellets. The pellet possesses the magnetic character of high magnetic conductivity, low coercive force and low magnetism left and so on. The invention relates to the supplication of processing the magnetic sorting material into the magnetic sorting pellet. The pellet and the magnetic particle marked by the biologic molecular compose the magnetic sorting system. It can sort all kinds of biologic samples, such as cell, subcellular substance, bacterium, virus, nucleic acid, protein and so on.

Description

technical field [0001] The invention belongs to the field of magnetic materials, and more specifically relates to magnetic materials applied to the separation of biological samples. Background technique [0002] With the rapid development of modern biotechnology, magnetic separation technology has been widely used in the separation of biological samples. The principle is: the surface modification of magnetic beads (particle size <5 μm) can recognize and bind cells and subcellular substances Biomolecules of biological samples such as bacteria, viruses, nucleic acids or proteins form magnetic bead-biomolecular complexes. The complex is used to capture the target biological sample, and then retained under the action of an external magnetic field to achieve separation. Due to the low magnetic induction intensity generated by the ordinary permanent magnet magnetic field, it is difficult to achieve fast and efficient separation of target isolates. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): H01F1/09H01F41/02C12N13/00C12Q1/24
Inventor 沈鹤柏周海清兰天张诗渊
Owner SHANGHAI NORMAL UNIVERSITY
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