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Method for degrading rice grass cellulose by multi-bacterium step fermentation

A stepwise, multi-strain technology, applied in animal feed, animal feed, application, etc., can solve the problems of low protein content of the transformant, harsh overall operating conditions, and low cellulose degradation rate, so as to increase protein content and prepare The effect of scientific and reasonable method and wide source of raw materials

Inactive Publication Date: 2007-03-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of them do not involve multi-stage stepwise fermentation methods. Since they have not been subjected to high-temperature fermentation treatment, bacteria and toxins cannot be removed, which brings inconvenience to microbial fermentation, resulting in low cellulose degradation rate and low protein content in the transformed products. ; The overall operating conditions are relatively harsh, not convenient enough, and there are more restrictions on technology promotion

Method used

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  • Method for degrading rice grass cellulose by multi-bacterium step fermentation

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Experimental program
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Effect test

Embodiment 1

[0018] The composition of the culture medium for dermatophilus DJSF-1 slant culture and preservation is (g / L): 2g peptone, MgSO 4 0.5g, K 2 HPO 4 1g, NaCl 0.5g, cellulose powder 10g, agar; pick the dermatophilus DJSF-1 bacterial classification in the corresponding culture medium, and the thalline growth medium consists of (unit is g / L): rice straw powder 30, bran Skin 5, soybean cake powder 5, urea 5, KH 2 PO 4 3.0, CaCl 2 2H 2 O 1.0, MgSO 4 ·7H 2 O1.0, cellulose powder 3.0; culture pH 5.5~7.0, temperature 50~70°C, 120~160rpm shaker culture for 4~5 days to obtain bacteria and fermented enzyme liquid; take 120Kg of mechanically crushed straw material, its The water content is 30%; Inoculate the enriched dermatophilus species and enzyme liquid into the above-mentioned rice straw material for accumulation and fermentation, press bacteria liquid: rice straw powder=1:50v / m, ferment at 60~65°C for 3 days The crude protein content in the fermentation product is 10.35%, and...

Embodiment 2

[0020] The composition of the culture medium for dermatophilus DJSF-1 slant culture preservation is (in g / L): peptone 2, MgSO 4 0.5,K 2 HPO 4 1. NaCl 0.5, cellulose powder 10, agar; pick Dermophilus DJSF-1 strains in the corresponding culture medium, and the bacterial growth medium consists of (g / L): straw powder 50, bran 10, soybean cake powder 7, urea 7, KH 2 PO 4 7. CaCl 2 2H 2 O3, MgSO 4 ·7H 2 O 3, cellulose powder 3; culture pH 5.5~7.0, temperature 50~70°C, 120~160rpm shaker culture for 4~5 days to obtain bacteria and fermented enzyme liquid; get 120Kg of mechanically crushed straw material, which contains The amount of water is 50%; the enriched dermatophilus species and enzyme liquid are inoculated into the above-mentioned rice straw material for accumulation fermentation, wherein bacterial liquid: rice straw powder = 1: 100v / m, fermented product after 5 days of fermentation at 60-65°C The crude protein content is 15.72%, and the ammoniacal nitrogen content i...

Embodiment 3

[0022] The composition of the culture medium for dermatophilus DJSF-1 slant culture preservation is (in g / L): peptone 2, MgSO 4 0.5,K 2 HPO 4 1. NaCl 0.5g, cellulose powder 10g, agar; pick the dermatophilus DJSF-1 bacterial classification in the corresponding culture medium, and the bacteria growth medium consists of (unit is g / L): rice straw powder 70, bran Skin 15, soybean cake powder 10, urea 10, KH 2 PO 4 10, CaCl 2 2H 2 O5, MgSO 4 ·7H 2O 5, cellulose powder 5; culture pH 5.5~7.0, temperature 50~70°C, 120~160rpm shaker culture for 4~5 days to obtain bacteria and fermentation enzyme liquid; get 120Kg of mechanically crushed straw material, which contains The amount of water is 80%; the enriched dermatophilus species and enzyme liquid are inoculated into the above-mentioned rice straw material for accumulation and fermentation, wherein the bacterial liquid: rice straw powder = 1: 200v / m, fermented at 60-65°C for 7 days and then fermented The medium crude protein c...

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Abstract

The present invention relates to a method capable of utilizing multistrain steped fermentation method to degrade cellulose in rice straw and raise nutritive value of fermented product. Said fermentation method includes two stages: first stage utilizes peelophile high-temperature fermentation to produce enzyme capable of degrading cellulose, decomposing cellulose into the useable carbon source, at the same time killing harmful bacteria; the second stage uses the cellulose degraded product produced in first stage as substrate, and utilizes torula yeast solid fermentation to produce single-cell protein. The fermented final product can be dried, pulverized and puffed so as to obtain the invented granular feed in which the protein content can be up to 16.84-23.24%.

Description

technical field [0001] The invention belongs to the technical field of deep processing of by-products of agricultural crops, and relates to the preparation of strains and a multi-strain stepped fermentation method to degrade cellulose in rice straw and improve the nutritional value of fermentation products. Background technique [0002] my country is a large agricultural country, and the total grain output has reached 450 million tons. With the continuous growth of grain production, the amount of by-products of agricultural products such as straw, wheat straw, corn stalk, peanut vine and other crop straws has also increased accordingly. The total output of straw, seedlings and vines used is close to 600 million tons, and only about 25% of this huge resource is currently used to directly raise livestock. [0003] So far, there are three methods for the treatment of straw: 1. Physical methods, such as crushing, puffing, soaking, cooking, thermal spraying, radiation and so on. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K10/12A23K10/37
CPCY02P60/87
Inventor 黄达明张志才崔凤杰吴春笃肖香钱静亚
Owner JIANGSU UNIV
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