Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste

Abstract

Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, following oil extraction, as a substrate for a Pseudomonas aeruginosa, Enterobacter hormaechei or Enterobacter buriae line cultivated in a bioreactor with a non-dispersive aeration system for reducing foam, producing a rhamnolipid content of 10.5 g / L for Pseudomonas aeruginosa bacteria, in bioreactors carried out in a stirred tank with non-dispersive aeration using microporous membranes, particularly of silicone tubes, which allow oxygen to be supplied by diffusion. This type of aeration allows for various configurations, and in the embodiment of the invention, the porous membrane / tube was internally located in the liquid in the bioreactor in the form of a serpentine, under the following process conditions: pure oxygen with suitable pressure and flow rate to maintain O2 pressure in the bioreactor at 20% during the first 24 hours of the assay and stirring varying from 300 to 700 rpm, using 2 radial impellers and manual adjustment according to the decrease in the concentration of dissolved oxygen. The product produced has features that can be used primarily in the cosmetic industry due to its emulsifying, stability and non toxicity capacities.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a national stage application, filed under 35 U.S. C. § 371, of International Application No. PCT / BR2018 / 050003, filed Jan. 8, 2018, which claims priority to Brazilian Application No. BR102017000578-0, filed Jan. 11, 2017, the contents of both of which as are hereby incorporated by reference in their entirety.BACKGROUNDTechnical Field[0002]The present invention, which belongs to the field of lipid compounds containing monosaccharide units, refers to biosurfactants / rhamnolipids of microbial origin obtained from Pseudomonas aeruginosa or Enterobacter hormaechei or Enterobacter buriae microbial strains and agroindustrial andiroba or murumuru seed residue as a substrate for their growth and production, and the process for obtaining the same, having features that can be applied in the cosmetics industry due to the emulsifying ability, stability and non-toxicity thereof.[0003]Biosurfactants are surface-active agents having am...

AI Technical Summary

Benefits of technology

[0037]“THE PROCESS for obtaining rhamnolipid from Pseudomonas and Enterobacter using the ANDIROBA or murumuru seed residue” refers to a process for obtaining rhamnolipid biosurfactant from Pseudomonas and Enterobacter using andiroba or murumuru seed residue as an alternative and renewable substrate to produce the rhamnolipid biosurfactant, which exhibits emulsifying properties, stability and non-toxicity and finds use in cosmetic formulations, which process comprises the steps of: a) cellular reactivation; b) preparation of an inoculum and c) batch bioprocessing in a bioreactor.

Problems solved by technology

In general, biosurfactants are still unable to compete with synthetic surfactants for commercial purposes owing to their high production and recovery costs.

Trends Biotechnol., Amsterdam, 24: 509-515, 2006), three main factors that make the commercialization of biosurfactants difficult are: i) high costs of raw materials; ii) the high recovery and purification costs; and (iii) low yields in the production processes.

Thus, the state of the art has demonstrated that bacteria from the Pseudomonas genus are able to produce different rhamnolipid family biosurfactants, starting from different substrates, however, there is no information available on the production of rhamnolipids from plant residues.

However, producing biosurfactant molecules having properties applicable to cosmetic products that are similar or superior than those of chemical surfactants is a great challenge.

Having knowledge on the suitable nutrient composition for cellular growth and buildup of the product of interest is one of the major issues found when selecting the residue to be used.

Establishment of a biotechnological process that uses such alternative substrates has also another difficulty, which is the standardization caused by natural variations of the composition, as well as transportation, storage and pre-treatment costs (NITSCHKE, M.; PASTORE, G. M. Biossurfactantes a partir de resíduos agroindustriais: Avaliação de resíduos agroindustriais como substratos para a produção de biossurfactantes por Bacillus.

However, a poorly evaluated factor that may contribute to an increased biosurfactant production is the influence of micronutrients present in the culture medium.

Biosurfactant production faces several processing difficulties.

Environmental factors and growth conditions such as pH, temperature, agitation and oxygen availability affect biosurfactant production, interfering with growth and cellular activity.

Images