Hydrogen sulfide releasing polymer compounds
a technology of hydrogen sulfide and polymer compounds, which is applied in the field of polymer compounds, can solve the problems of difficult administration of hydrogen sulfide, and inability to control the release of hydrogen sulfide by hydrolysis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 4
[0132]The investigated polymer compounds according to the invention (as shown in FIGS. 1A and 1C) were analyzed for their hydrogen sulfide release properties in presence of a thiol containing component with an amperometric detection device (H2S Micro-Sensor with H2S permeable membrane; AMT Analysenmesstechnik GmbH). The H2S release was triggered by the addition of L-cysteine.
[0133]FIG. 6 depicts the results of a H2S release assay, wherein 50 mL of a 0.1% (m / m) solution of the H2S releasing chitosan derivative, which was synthesized as described in example 1, was prepared with 50 mM acetate buffer (pH 5.5). To the resulting solution, which had a pH of 5.3, 1.5 mL of a 100 mM solution of L-cysteine were added. The resulting release of H2S was monitored at room temperature.
[0134]FIG. 7 depicts the results of a H2S release assay, wherein 0.1% (m / m) of a H2S releasing hyaluronic acid derivative of example 2 (FIG. 1A) were incubated in DMEM (Dulbecco's modified eagle med...
example 6
ling Preparation—Sponge
[0138]An aqueous hydrogel solution comprising 1.5% (m / m) H2S releasing chitosan derivative with a degree of modification of about 185 mol S-acetyl moieties according to the invention is prepared. The solution is cast into 12 well cell culture plates-in an amount of 0.9 g per well) and freeze dried. The resulting lyophilisate is a sponge with a round shape and has a diameter of about 2 cm. The sponges are sealed individually in pouches. The pouches and secondary packaging material are then sterilized via gamma irradiation.
[0139]For the treatment of wounds the sterile sponges are applied on the skin wound. A few drops of a sterile buffered solution of N-acetylcysteine in a concentration of 5 mg / mL may be applied on the sponge to immediately start the release of H2S. The wound area is then covered with a secondary wound dressing with low gas permeability, such as a film consisting of polyethylene, polypropylene or polytetrafluoroethylene or a fabric or fleece tha...
example 7
tment—Topical Hydrogel
[0140]An aqueous hydrogel solution comprising 1% (m / m) H2S releasing hyaluronic acid derivative with a degree of modification of about 250 mol S-acetyl moieties according to the invention is prepared in a phosphate buffer with a physiologically acceptable pH value. The solution is then filled into single use containers (e.g. single dose polypropylene containers or glass syringes with a filling volume of about 3 mL) and sterilized with moist heat. A second composition comprising N-acetylcysteine in a physiologic buffer solution in a concentration of 10 mg / mL is also provided in a single use container. The resulting hydrogel with a low viscosity is applied on intact skin or inflamed skin areas, immediately followed by the application of approximately the same amount of the composition comprising N-acetylcysteine to the same skin area.
[0141]The treatment with the hydrogel may be preferred in case of cosmetic skin conditions or skin disease such as for treatment of...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More