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Method for preparing pollen pini polyose, pollen pipe polyose and its use in medicine

A technology of pine pollen and polysaccharides, which is applied in the direction of drug combinations, medical preparations containing active ingredients, and pharmaceutical formulas, to achieve the effects of improving immunity, improving blood sugar and blood pressure, and lowering blood sugar and blood pressure

Inactive Publication Date: 2008-09-10
HAINAN JINXING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The relevant effects of these pine pollen polysaccharides have not been reported

Method used

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  • Method for preparing pollen pini polyose, pollen pipe polyose and its use in medicine
  • Method for preparing pollen pini polyose, pollen pipe polyose and its use in medicine
  • Method for preparing pollen pini polyose, pollen pipe polyose and its use in medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the preparation of pine pollen polysaccharide

[0027] (1) Extraction: Take 2kg of broken-walled pine pollen, add 60L of water, stir and heat up to 95°C-100°C in a heating extraction tank, stir and extract at a constant temperature for 8 hours, and drop to 40-45°C.

[0028] (2) Impurity removal: adjust the pH to 8.0-8.5 with NaOH, add trypsin with 3% weight of pine pollen, incubate at 40-45°C for 4 hours, then heat up to 95°C, maintain for 15 minutes, and then lower to room temperature Adjust the pH to 5.5-6.0 with HCl, add cellulase with 3% weight of pine pollen, incubate at 40-45°C for 4 hours, heat up to 95°C, maintain it for 15 minutes, and cool down to room temperature. Centrifuge at a high speed with a relative centrifugal force of 9100g, collect the supernatant, and ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 100kd to collect the non-filtrate.

[0029] (3) Alcohol precipitation: the non-filtrate was heated and co...

Embodiment 2

[0032] Example 2: Effect of pine pollen polysaccharide on blood pressure level in renally hypertensive rats

[0033] 1. Experimental animals and group settings

[0034] Wistar rats, 9-10 weeks old, weighing 200±20g, male, were randomly divided into a blank control group, a model control group, and experimental drug groups I and II, with 10 rats in each group.

[0035] 2. Preparation of hypertensive rat model

[0036] Use diazepam 5mg / kg and ketamine 50mg / kg, intradermally anesthetize rats, fix in supine position, after skin disinfection, incise the skin and muscle layer along the midline of the abdomen at 1.5cm below the xiphoid process, gently squeeze out the kidney, Wrap it with saline cotton and fix it with your left index finger and thumb. Use the right hand to carefully separate the fascia of the renal pedicle with straight ophthalmic tweezers without hooks, separate the renal artery along the lower part of the renal vein, wrap it with an H-shaped silver clip (0.20-0.25...

Embodiment 3

[0053] Example 3: Effect of pine pollen polysaccharide on blood lipid levels in rats with dyslipidemia

[0054] 1. Animal selection and group setting

[0055] With embodiment 2.

[0056] 2. Rat hyperlipidemia model preparation

[0057] Rat hyperlipidemia model adopts the method of hyperlipidemia induced by high-fat diet. The formula of high-fat feed is as follows: 86.3% of basic feed, 3% of cholesterol, 10% of lard, 0.2% of methylthiouracil, and 0.5% of pig bile salt, so as to ensure that the ingredients are mixed evenly. Continuous feeding for 2 weeks, anesthetized with pentobarbital sodium solution, tail blood was taken, and blood lipids were detected, which proved that the model was successfully manufactured.

[0058] 3. Experimental method

[0059] Blank control group: no modeling, no drug administration, and an equal amount of normal saline;

[0060] Model control group: modeling, no drug administration, and high-fat feed every morning;

[0061] Experimental drug gr...

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Abstract

The process of preparing pollen pini polyose includes the following steps: 1. decocting wall broken pollen pini to extract and filtering; 2. enzymolyzing with pancreatin and cellulose, centrifuging at high speed and ultrafiltering to eliminate impurity; 3. alcohol precipitating the filter residue liquid, and drying the precipitate to obtain coarse pollen pini polyose; 4. eluting coarse pollen pini polyose in DEAE-cellulose column; and 5. nanofiltering, concentrating the filter residue liquid, drying and crushing to obtain single pollen pini polyose. The present invention also discloses the medicinal application in preparing medicine for lowering blood fat, blood pressure and blood sugar, resisting fatigue and raising immunity. The present invention provides one new way for developing and utilizing pollen pini.

Description

technical field [0001] The invention relates to pine pollen polysaccharide and its preparation method, and its application in pharmacy, especially the application of pine pollen polysaccharide in the preparation of medicines for lowering blood fat, lowering blood pressure, lowering blood sugar, anti-fatigue and improving immunity. Background technique [0002] With the rapid development of my country's economy and society and the continuous improvement of people's living standards, people's eating habits have changed, bad habits have increased, the pace of work has accelerated, and social pressure has increased. These factors lead to common sub-health problems in people's bodies, such as excessive fatigue, accelerated aging, decreased immunity, high blood pressure, high blood fat, high blood sugar, etc. This is easy to induce diseases such as cerebral thrombosis, cerebral hemorrhage, coronary heart disease, diabetes, etc. that are difficult to be completely cured and sudden ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/718A61P3/06A61P3/10A61P37/02
Inventor 刘全胜夏中宁舒军林学良
Owner HAINAN JINXING PHARMA
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