Goose sperm freezing dilution liquid kit and using method

A technology for freezing diluents and kits, applied in biochemical equipment and methods, applications, microorganisms, etc., can solve the problems of feasibility, inconvenience, complicated operation methods, etc., to achieve simple operation, improve use effect, use handy effect

Inactive Publication Date: 2007-09-19
徐日福 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Based on the above domestic and foreign research data, it is not difficult to find that many semen freezing effects are unstable or even poor. This is mainly due to the following four deficiencies or defects in the existing semen freezing techniques:
Lack of screening in the formulation and compatibility of basic diluents
[0019] (2) There is no pre-conducted toxicity test on the selection and concentration of cryoprotectants, with a certain degree of blindness
[0020] (3) There is no experimental basis for the compatibility and ratio of basic diluent and cryoprotectant
[0021] (4) Some freezing procedures or technical processes are unreasonable, and the operation methods are too complicated and inconvenient to use
For example, freezing equilibrium time, equilibrium temperature, and freezing must be tested for specificity; for example, the required low temperature (5°C) centrifugation and dialysis, etc., are applied in production, and the feasibility will be affected.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] In the technology extension base, operators use this kit. In the kit, the basic nutrient dilution powder is composed of: 0.55g sodium glutamate, 2.0g potassium dihydrogen phosphate, 0.36g dipotassium hydrogen phosphate, and 0.07 g cysteine. g, fructose 0.11g, inositol 0.05g, raffinose 0.6g, Tris 0.2g, penicillin sodium 100,000 units, streptomycin sulfate 0.09g, calcium chloride 0.01g; protamine sulfate, 0.042g; DMA5. 0g and ethylene glycol 4.5ml. Prepare frozen dilutions according to the kit instructions.

[0095] The gander semen was collected by back massage method. During the collection process, the semen is required to be free from contamination such as feces, blood and feed. After collection, wrap them in cotton, place them in a thermos cup, and quickly bring them back to the laboratory for processing. And strictly follow the "two-step method" semen freezing procedure established above, that is, ① the first step of dilution: after the semen is collected, put it ...

Embodiment 2

[0100] In the relevant units of technology promotion, the operator is equipped with frozen diluent according to the instructions of the kit. The gander semen was collected by back massage method. And in strict accordance with the "two-step method" semen freezing procedure established above, the goose semen was frozen. The operator follows the method and operation steps in Example 1 to thaw frozen semen particles, check and record sperm motility, and perform artificial insemination, egg collection, preservation and artificial incubation after thawing of frozen semen. The inseminated parental geese were 1200, 390 days old, healthy and disease-free, with high egg production rate. After insemination, the results are shown in Table 2:

[0101] Insemination batch

[0102] It can be seen from Table 2 that the range of sperm motility after thawing of frozen semen granules is 0.44-0.70, and the fertilization rate of breeding eggs is 84.7-86.2%. Both technical indicators are...

Embodiment 3

[0104] In the relevant units of technology promotion, the operator prepared the frozen diluent according to the instructions of the kit. The gander semen was collected by back massage method. And in strict accordance with the "two-step method" semen freezing procedure established above, the goose semen was frozen. The operator follows the method and operation steps in Example 1 to thaw frozen semen particles, check and record sperm motility, and perform artificial insemination, egg collection, preservation of artificial incubation, records and statistics after the frozen semen has been thawed. The inseminated parental geese were 1500, 330 days old, healthy and disease-free, with high egg production rate. After insemination, the results are shown in Table 3:

[0105] Insemination batch

[0106]It can be seen from Table 3 that the range of sperm motility after thawing of frozen semen granules is 0.43-0.69, and the fertilization rate of breeding eggs is 83.9-85.1%. I...

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PUM

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Abstract

A case of goose seminal fluid chill diluent and application method thereof belongs to animal breed technique field, the reagent case including: component I: sodium glutamate 0.4-0.6, potassium dihydrogen phosphate 1.4-2.2, dipotassium hydrogen phosphate 0.2-0.5, homocycteine 0.0006-0.01, fructose 0.9-1.2, inositol 0.04-0.08, raffinose 0.5-0.8, sodium oxacillin 8-12, streptomycin sulphate 0.07-0.15, calcium chloride 0.01-0.02, 3-carboxymethylaminomethane 0.1-0.3; component II: double distilled water 100; component III: protamini sulfas 0.04-0.06 g; component IV: DMA 4.5-5.5, glycol: 4.5-5.5 ml. Useful effects: using components of 3-carboxymethylaminomethane and protamini sulfas and quantifying accurately. Compounding each component of goose seminal fluid chill diluent as packing type of case firstly. The method is simple and easy to operate, and improve applying effects of the case.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction. Background technique [0002] Semen cryopreservation is a major innovation in artificial insemination technology. It solves the problem of long-term storage of semen, so that the semen is not limited by time, region and the life of the breeding stock. It can make full use of the genetic performance of the best breeding breeds, maximize the use of fine breeding male geese, and greatly improve Genetic progress accelerates the pace of breeding and improvement of varieties. At the same time, it is of great significance and application to conduct short-term descendant determination of fine male geese, to preserve and restore the excellent characteristics of a certain breed or individual male geese, and to update bloodlines, introduce species, reduce production costs, and protect species resources. value. [0003] Over the past 50 years, domestic and foreign scholars and animal reproduct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A01N1/02
Inventor 徐日福吴伟高光崔柏勋孙永峰
Owner 徐日福
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