Goose sperm freezing dilution liquid kit and using method

Abstract

A case of goose seminal fluid chill diluent and application method thereof belongs to animal breed technique field, the reagent case including: component I: sodium glutamate 0.4-0.6, potassium dihydrogen phosphate 1.4-2.2, dipotassium hydrogen phosphate 0.2-0.5, homocycteine 0.0006-0.01, fructose 0.9-1.2, inositol 0.04-0.08, raffinose 0.5-0.8, sodium oxacillin 8-12, streptomycin sulphate 0.07-0.15, calcium chloride 0.01-0.02, 3-carboxymethylaminomethane 0.1-0.3; component II: double distilled water 100; component III: protamini sulfas 0.04-0.06 g; component IV: DMA 4.5-5.5, glycol: 4.5-5.5 ml. Useful effects: using components of 3-carboxymethylaminomethane and protamini sulfas and quantifying accurately. Compounding each component of goose seminal fluid chill diluent as packing type of case firstly. The method is simple and easy to operate, and improve applying effects of the case.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction. Background technique [0002] Semen cryopreservation is a major innovation in artificial insemination technology. It solves the problem of long-term storage of semen, so that the semen is not limited by time, region and the life of the breeding stock. It can make full use of the genetic performance of the best breeding breeds, maximize the use of fine breeding male geese, and greatly improve Genetic progress accelerates the pace of breeding and improvement of varieties. At the same time, it is of great significance and application to conduct short-term descendant determination of fine male geese, to preserve and restore the excellent characteristics of a certain breed or individual male geese, and to update bloodlines, introduce species, reduce production costs, and protect species resources. value. [0003] Over the past 50 years, domestic and foreign scholars and animal reproduct...

AI Technical Summary

Problems solved by technology

[0017] Based on the above domestic and foreign research data, it is not difficult to find that many semen freezing effects are unstable or even poor. This is mainly due to the following four deficiencies or defects in the existing semen freezing techniques:

Lack of screening in the formulation and compatibility of basic diluents

[0019] (2) There is no pre-conducted toxicity test on the selection and concentration of cryoprotectants, with a certain degree of blindness

[0020] (3) There is no experimental basis for the compatibility and ratio of basic diluent and cryoprotectant

[0021] (4) Some freezing procedures or technical processes are unreasonable, and the operation methods are too complicated and inconvenient to use

For example, freezing equilibrium time, equilibrium temperature, and freezing must be tested for specificity; for example, the required low temperature (5°C) centrifugation and dialysis, etc., are applied in production, and the feasibility will be affected.