Method for producing hydrogen by using marsh red pseudomonas

A swamp rhodopseudomonas, hydrogen production reactor technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to guarantee photosynthetic bacteria, high hydrogen production capacity, and lack of simultaneous provision of light energy, etc. , to achieve the effect of broad sustainable utilization, reduction of organic content and low cost

Inactive Publication Date: 2007-09-26
CHONGQING UNIV
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AI Technical Summary

Problems solved by technology

[0005] At present, there is no literature report on the method of specifically cultivating hydrogen-producing photosynthetic bacteria. At the same time, more literature on the screening of photosynthetic bacteria focuses on the proliferation and expansion of photosynthetic bacteria. What is obtained is the photosynthetic bacteria culture solution containing miscellaneous bacteria. Most of the photosynthetic bacteria are only used for the production of feed or protein or sewage treatment, and there is no close connection between organic waste treatment and energy. Some sources report that the screening of photosynthetic bacteria uses anaerobic incubators, but this The method only provides anaerobic conditions without providing light energy at the same time, so it cannot guarantee that the obtained photosynthetic bacteria have high hydrogen production capacity

Method used

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  • Method for producing hydrogen by using marsh red pseudomonas
  • Method for producing hydrogen by using marsh red pseudomonas
  • Method for producing hydrogen by using marsh red pseudomonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the cultivation of Rhodopseudomonas palustris:

[0031] (1) Collection of bacteria source: Collect the upper surface silt of farmland and sewage ditches as the bacteria source, let it stand for 5 hours, and collect the supernatant; under the microscope, a preliminary microscopic examination of the type and quantity distribution of microorganisms found a large number of other miscellaneous bacteria , such as Candida, green algae, etc., the number of photosynthetic bacteria is very small;

[0032] (2) Proliferation and cultivation of Rhodopseudomonas palustris strains with hydrogen production behavior: Take 70ml of the above supernatant and pour them into 250ml Erlenmeyer flasks, add 30ml of sterilized fresh culture-based flasks at the same time, and put the supernatant Shake well with fresh culture medium, fill with argon gas to remove air, carry out static anaerobic culture, use fluorescent lamp as light source in bacterial culture, illuminance is 3000 lux...

Embodiment 2

[0039] (1) Production of the photobiological hydrogen production reactor: select transparent plexiglass as the material of the photobiological hydrogen production reactor, the thickness of the plate is 0.8cm, the length of the reactor is 10cm, the width is 5cm, and the height is 10cm. The reactor is a cuboid structure. The total volume of the reactor is 500ml, and a hole is opened on the left and right sides of the top of the reactor for filling argon and discharging air, and a hole is opened near the bottom of the reactor for liquid sampling and draining. The joint is connected to the outside, and the exhaust hole is connected to the external gas collection device for collecting hydrogen. The reactor should not leak gas or liquid except for the necessary openings;

[0040] (2) Preparation of hydrogen-producing liquid medium formula:

[0041] Glucose: 7.92g / l; K 2 HPO 4 ·3H 2 O: 0.6g / l;

[0042] K H 2 PO 4 : 0.38g / l; NH 4 Cl: 1.99g / l;

[0043] CaCl 2 : 0.1g / l; FeSO 4...

Embodiment 3

[0055] (1) liquid medium preparation: get glucose 19.8g / l; K 2 HPO 4 ·3H 2 O: 0.6g / l;

[0056] K H 2 PO 4 : 0.38g / l; NH 4 Cl: 1.99g / l;

[0057] CaCl 2 : 0.1g / l; FeSO 4 ·7H 2 O: 0.0834g / l;

[0058] ZnSO 4 : 0.0005g / l; MgSO 4 ·7H 2 O: 0.05g / l;

[0059] NaCl: 1.0g / l; CuSO 4 : 0.03g / l

[0060] (NH 4 ) 6 Mo 4 o 24 4H 2 O: 0.00075g / l; V B2 : 0.00002g / l;

[0061] Niacin: 0.00002g / l; beef extract: 3g / l;

[0062] Water: 1000ml; growth factor solution: 2ml;

[0063] Adjust the initial pH of the liquid medium to 8.0

[0064] Other conditions and steps were the same as in Example 2, and it was found that Rhodopseudomonas palustris began to produce hydrogen at 12 hours after inoculation and culture, and the hydrogen production rate was 0.5376ml h -1 l -1 , at this time the biomass (dry weight) is 0.037g l -1, when cultured to the 23rd hour, the hydrogen production rate reached the maximum, which was 14.0672ml·h -1 l -1 , the biomass is 0.28g·l -1 . From then ...

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Abstract

The invention discloses a hydrogen preparing method with marsh red pseudomonas, which comprises the following steps: (1) putting fluid medium into autoclave sterilizing pot; sterilizing at 12-15 min; fetching out of the culture medium; cooling to normal temperature; embedding the fluid medium into bioreactor and photo biological hydrogen production reactor separately; setting the initial pH range at 4.5-8.8 and density range of organic subtract at 1.98g/l-45g/l; (2) fetching marsh red pseudomonas colony on solid double layer plate culture medium with inoculating needle; seeding in the cooling culture medium; charging into argon gas to remove air in the biological reactor of fluid medium; forming anaerobic environment; placing the seeding liquid medium at 25-35 deg.c; stewing and culturing at 36-96 h in constant-temperature culture box with illuminance at 1500lx-8000lx; getting the enlargement culture liquid in the subsequent hydrogen producing course.

Description

technical field [0001] The invention relates to a method for producing hydrogen by microorganisms, in particular to a method for producing hydrogen by using Rhodopseudomonas palustris. Background technique [0002] At present, the annual output of hydrogen in the world is more than 36 million tons, mainly from fossil fuels, biomass and water, mainly using traditional physical and chemical methods such as water electrolysis, catalytic reforming and thermal cracking of natural gas, coal gasification, and naphtha Oxidation, etc. to produce hydrogen, the traditional hydrogen production method is to consume a large amount of fossil energy in exchange for hydrogen energy, the net energy value is low, the economic applicability is not strong, and at the same time, it pollutes the environment. Hydrogen can also be produced by combining photoelectric technology and electrolysis technology. Using solar photovoltaic cells or solar thermal collectors can produce higher conversion effici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/38
Inventor 廖强王永忠朱恂田鑫石泳丁玉栋
Owner CHONGQING UNIV
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