Method for preparing enzyme of dissolving staphylococcal bacteria, its derivative, and method for preparing the derivative

Abstract

This invention relates to a method for preparing lysostaphin and its derivatives. The method comprises: designing and synthesizing lysostaphin gene, inserting lysostaphin gene into the digestion site of expression vector (plasmid pET), transforming E. coli, screening positive clones, culturing, inducing the expression of target protein, collecting E. coli, lysing, centrifuging, collecting the supernatant, and purifying the supernatant through chromatography. The method has such advantages as simple process, high expression level, simple purification, high yield, soluble product, and no requirement for renaturation, and is suitable for mass production. The lysostaphin derivatives are obtained by modifying lysostaphin with polyethylene glycol, and have such advantages as low immunogenicity and long half value period. The lysostaphin and its derivatives are suitable for treating Staphylococcus infection in clinic.

Description

technical field The invention relates to the field of biotechnology, in particular to a preparation method of lysostaphin and its derivative and the preparation method of the derivative. Background technique Staphylococcus (Staphylococcus) is a kind of Gram-positive coccus, which is widely distributed in nature, such as air, water, soil, feed and some articles, and also exists on the body surface, nasopharynx and intestinal tract of humans and animals. Most of them are not pathogenic, and a few can cause human and animal pathogenicity. Among them, Staphylococcus aureus is the most pathogenic, often causing purulent inflammation of the skin, tissues and organs, and is the cause of burn wound infection, acute liver failure and blood infection. The main pathogenic bacteria of diseases such as primary nephritis. Staphylococcus aureus also occurs simultaneously with G-sepsis bacteria, acting synergistically, seriously threatening the lives of patients. Enterotoxin produced by S...

AI Technical Summary

Problems solved by technology

The yield of expressing lysostaphin in the prior art cannot meet the requirements of large-scale preparation, and the cost of PEG modification is very high, so it is necessary to develop a method suitable for large-scale preparation of lysostaphin and a low-cost modification technology

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