Immune syntony scattering spectrometry for detecting B factor
A technology of resonance scattering and factor B, applied in the field of immune resonance scattering spectroscopy for detecting factor B, can solve the problems of long operation process, high cost, complicated operation, etc., and achieves less sample consumption, short measurement time and high sensitivity. Effect
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Embodiment 1
[0029] 1. Prepare a test system with known factor B concentration, add Na 2 HPO 4 -NaH 2 PO 4 Buffer solution (PBS) 0.40 mL, goat anti-human factor B solution (dilution ratio 1:20, Shanghai Beika Reagent Co., Ltd.) 200 μL, 12 μg / mL factor B antigen (Shanghai Beika Reagent Co., Ltd.) 20, 140, 260 , 380, 500, 620, 740μL, shake well, then add 0.40mL of 15% PEG6000 solution, add double distilled water to make up to 3.0mL, shake well, put the stoppered test tube in 37℃ water bath for 5 minutes, take out the running water to cool ;
[0030] 2. According to the method in step 1, but without adding factor B antigen, prepare a reagent blank control system;
[0031] 3. the above-mentioned system gained solution is poured in the quartz cuvette respectively, is placed on the Cary Eclipse fluorescence spectrophotometer (U.S. Varian company) at λ ex -λ em = 0 conditions, synchronous scanning to obtain the resonance scattering spectrum of the system (as shown in Figure 1), measure the ...
Embodiment 2
[0037] 1. Add pH 7.2 Na 2 HPO 4 -NaH 2 PO 4 0.40 mL of buffer solution, 200 μL of goat anti-human factor B solution (dilution ratio 1:20, Shanghai Beika Reagent Co., Ltd.), 12 μg / mL factor B antigen (Shanghai Beika Reagent Co., Ltd.) 50, 100, 200, 400, 800 μL, shake well, then add 0.50 mL of 15% PEG6000 solution, add twice distilled water to make up to 3.0 mL;
[0038] 2. Place the stoppered test tube in step 1 in a 37°C water bath, and after reacting for 5 minutes, take out the running water to cool;
[0039] 3. the solution gained in step 2 is poured in the quartz cuvette, placed on the Cary Eclipse fluorescence spectrophotometer (U.S. Varian company) at λ ex -λ em =0 conditions, synchronously scan to obtain the resonance scattering spectrum of the system, and simultaneously measure the resonance scattering intensity I at the wavelength of 400nm;
[0040] 4. Take no B factor antigen as the reagent blank, and the measured resonance scattering intensity is the blank valu...
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