Cosmetic product betamethasone high efficiency liquid chromatography detection method

Abstract

The invention provides an analysis method of detecting the betamethasone in cosmetic by use of high efficiency liquid chromatographic instrument, and the invention is characterized in that octadecylsilane chemically bonded silica is adopted as the C18 column or the C8 column of filler, and an ultraviolet detector or a diode array detector are used to detect at the position of 190 to 380 nm; the mobile phase adopts methanol-water mixed solution or acetonitrile-water mixed solution; the sample is extracted by methanol-hydrochloric acid mixed solution or acetonitrile-hydrochloric acid mixed solution, extracted by ultrasound for around 20 minutes, and undergone with an ice bath, and then is centrifuged in 12000rpm / min for 5 minutes. The clear liquid is prepared, which is, if necessary, filtered by 0.45Mu meters filtering membrane for using. The qualitative analysis is conducted according to the sample retention time and the spectrogram, and the quantitative analysis is conducted according to the peak area. The invention has the advantages of high solution and sensitivity, and perfect reproducibility and selectivity as well as simple sample pre-treating method, thereby being applicable to microanalysis of betamethasone in cosmetic.

Description

technical field [0001] The invention is a new technology for detecting betamethasone, a prohibited ingredient in cosmetics, by using high-performance liquid chromatography, and is especially suitable for qualitative and quantitative analysis of betamethasone in cosmetics. Background technique [0002] Betamethasone belongs to the class of adrenal corticosteroids and has antibacterial and anti-inflammatory effects. The 2002 edition of the "Hygienic Standards for Cosmetics" promulgated by the Ministry of Health stipulates that betamethasone must not be added to cosmetics. Due to its broad-spectrum and high-efficiency bactericidal effect, betamethasone is added to anti-acne products to achieve the purpose of anti-inflammatory, anti-acne, anti-mite and anti-acne. However, this drug has many adverse reactions, such as sodium excretion and strong inhibitory effect on growth; it can also cause various adverse reactions caused by corticosteroids, such as musculoskeletal, gastrointe...

AI Technical Summary

Problems solved by technology

The polarimetric method has many influencing factors, requires a large amount of standard reference substances and samples, and is not suitable for the analysis of trace amounts of betamethasone; the spectrophotometric method is cumbersome to operate, and there are many losses during sample processing, and the detection limit is high and the repeatability is high. Poor; high performance liquid chromatography is a more commonly used method for detecting betamethasone in recent years, but it is only limited to the detection of the content in raw materials and various pharmaceutical preparations. Less, and the sample pretreatment is simple and the loss of the target detection substance is small, so the content of betamethasone in the drug is easy to detect

However, the detection countries of the content of betamethasone in cosmetics, feed and meat have not formulated relevant detection standards and methods, and so far there is no relevant literature report on the detection of betamethasone in cosmetics

Different from pharmaceuticals, cosmetics have a wide variety of dosage forms such as ointment, cream, milk, powder, water, jelly, gel, etc., and the matrix is ​​complex, the physical and chemical properties of each component are quite different, and there are many interfering factors, especially betamethasone It is added to cosmetics as a banned ingredient in cosmetics, and its content is low. It is difficult to accurately detect trace amounts of betamethasone in cosmetics by the above-mentioned optical rotation method and spectrophotometry.

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