Preparation method for pig blood vessel acellular bracket by chemical and physical combination
A physical combination and decellularization technology, which is applied in the field of medical prosthesis materials, can solve the problems of high cost of decellularized scaffolds, inability to remove cell components, high use and maintenance costs, and achieve complete cell removal and easy cleaning of surfactants , The effect of low equipment cost
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[0021] The preparation method of the chemical and physical combination of the porcine blood vessel decellularized scaffold of the present invention is the preferred result after various decellularization method experiments. The ultrasonic disruptor in the physical method and the chemical surfactant in the Tira Take X-100 as an example to further describe the method of the present invention in detail as follows:
[0022] A) Preparation: After successful anesthesia of healthy pigs, remove the pig’s ascending aorta, aortic arch and coronary arteries not shorter than 5mm according to the requirements of aseptic operation, preserve the anterior leaflet of the mitral valve and the interventricular septal muscle with a thickness of about 3mm, and rinse with saline, respectively Measure the inner diameter of the artery at 10mm and 30mm on the annulus. After washing with PBS buffer, put it into a carbon dioxide dry ice incubator for disinfection. Take out the sterilized material on the ult...
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