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Application of locust glycolipid in preparing anti-skin ringworm medicament

A technology of dermatophytes and sophorolipids, which is applied in the application field of sophorolipids in the preparation of anti-dermatophyte drugs, can solve the problems of exacerbation of the disease, single species, elevated blood pressure, etc., and achieve simple and convenient process, low cost, Inexpensive effect

Inactive Publication Date: 2010-04-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet, through literature and patent retrieval, the research that utilizes the sophorolipid biosurfactant that microorganism produces to be used for preparing anti-dermatophyte (especially fungus) medicine has not been reported yet
[0004] At present, clinically, the infections caused by dermatophytes are increasing day by day, and the drugs used to treat dermatophytes are single. Many patients choose to take orally or inject corticosteroids under the torture of pain. swelling, high blood pressure, dizziness and other side effects, leading to aggravation of the condition

Method used

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  • Application of locust glycolipid in preparing anti-skin ringworm medicament
  • Application of locust glycolipid in preparing anti-skin ringworm medicament
  • Application of locust glycolipid in preparing anti-skin ringworm medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Preparation of Sophorolipid

[0049] (1) Selection of strains: Wickerhamiella domercqiae var.sophorolipid CGMCC No.1576;

[0050] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add 1.6% agar by mass to volume, and culture for 24 hours at 25°C;

[0051] (3) Seed culture: The strains cultivated in step (2) are connected with inoculation loops to 1-2 loops under aseptic conditions in 20 mL of medium containing 4% glucose and 1% rapeseed oil by mass volume , And shake culture for 24 hours at 25°C to prepare seed liquid;

[0052] (4) Expanded culture: inoculate 100 mL of the medium containing rapeseed oil with a mass-volume ratio of 1% with a 5% inoculum volume ratio, and shake culture for 96 hours at 25°C;

[0053] (5) Collect the fermentation product: the fermentation broth obtained in step (4) is allowed to settle, and the light yellow viscous liquid deposited on the bottom of the shake flask is collected, and then the above viscous substance...

Embodiment 2

[0056] Example 2: Preparation of Sophorolipid

[0057] (1) Selection of strains: Wickerhamiella domercqiae var.sophorolipid CGMCC No.1576;

[0058] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass-volume ratio of 1.6%, and culture at 30°C for 36 hours;

[0059] (3) Seed culture: The strains cultivated in step (2) are connected to 1 to 2 loops under aseptic conditions with inoculation loops in 50 mL of medium containing 8% glucose and 3% rapeseed oil by mass volume ratio , 30 ℃ conditions, shaking culture for 36 hours, to prepare seed liquid;

[0060] (4) Expanded culture: inoculate the seed liquid in 500 mL of medium containing rapeseed oil with a mass-to-volume ratio of 3% at an inoculum of 7% by volume, and shake culture for 200 hours at 30°C;

[0061] (5) Collect the fermentation product: The fermentation broth obtained in step (4) is allowed to settle, and the light yellow viscous liquid deposited on the bottom of the shake flask is co...

Embodiment 3

[0064] Example 3: Application of Sophorolipid as an antidermatophyte drug for inhibiting Trichophyton rubrum

[0065] Preparation of Trichophyton rubrum: An inoculation loop of Trichophyton rubrum preserved on the slope was transferred to a liquid Sabouraud medium, and cultured at 37°C for 20 hours to prepare a Trichophyton rubrum suspension.

[0066] The sophorolipid prepared in Example 1 was dissolved in dimethyl sulfoxide to prepare a mother liquor for use.

[0067] The mother liquor of sophorolipid was added to Sabo's solid medium, and the plate was poured and the final concentration of each dish was 0.1-5.0 g / L. Take 0.1 mL of the Trichophyton rubrum suspension and spread it on the solid plate medium with gradient concentration, and cultivate it at 35° C. for 120 hours. Observe the growth of dermatophytes on the plate and calculate the average number of colonies.

[0068] The formula of the above-mentioned liquid Sabol's medium (for fungal culture) is: peptone 10g / L, glucose 40g / ...

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Abstract

The invention discloses an application of sophorolipids in preparing drugs for resisting dermatophyte, and an application of a combination where the sophorolipids is used as an effective component inpreparing drugs for resisting dermatophyte. With low price, little dosage and remarkable antibacterial effect, the invention has no side effect on the human, and in certain aspects can be used as a drug for replacing conventional antifungal drugs which produce heat resistance easily and drugs for treating skin tinea disease such as corticosteroid with severe side effects. Thus, the invention has great application prospect.

Description

Technical field [0001] The invention relates to the application of the biosurfactant sophorolipid produced by fermentation of Wickerhamiella domercqiae var.sophorolipid, and in particular to the application of sophorolipid in the preparation of anti-dermatophyte drugs. Background technique [0002] Sophorolipid is an important biosurfactant. The research on it began in the 1950s and 1960s, and it was mainly obtained through microbial fermentation. Compared with chemically synthesized surfactants, sophorolipids have many advantages such as excellent surface properties and environmental friendliness. [0003] In recent years, studies on the antibacterial effect of sophorolipids have been reported. The applicant has also conducted preliminary studies on the effect of sophorolipids on bacteria. In January 2007, he applied for the Chinese invention patent "Sophorolipids in Application in the preparation of antibiotic drugs, patent number 200710013200.0". However, after literature and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7024A61K9/06A61P17/00A61P31/10
Inventor 宋欣曲音波邵凌健马晓静李慧
Owner SHANDONG UNIV
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