Method for preparing asiatic centella total saponins by using macroporous adsorption resin

Abstract

The invention discloses a method for utilizing the macroporous adsorption resin to prepare the total saponins of the herba centellae. The operation procedures of the method is carried out as follows, 1) aqueous ethanol solution is added into the dry herba centellae for immersion and extraction, the extracting solution is mixed and removed ethanol by the vacuum concentraction, and the concentratedsolution is diluted by water and filtered to obtain the aqueous total saponins of the herba centellae solution. 2), the pretreated macroporous adsorption resin is filled into the chromatography column. 3), the aqueous total saponins solution is introduced to flow through the resin column till penetration of the total saponins in the effluent. 4), the resin column is washed by water and eluted by the organic solvent-water mixture, the eluent is mixed after the thin layer chromatographic analysis, then is concentrated, crystallized and vacuum dried to obtain the total saponins product. 5), the resin column is washed and regenerated by the organic solvent-water mixture and water for reuse. The invention has the advantages of the higher absorption capacity of the total saponins of the herba centellae by the macroporous adsorption resin, easy desorption, higher recovery rate and higher contents of the total saponins of the herba centellae, simple method, strong operability, higher yield ofthe total saponins of the herba centellae and lower cost.

Description

technical field [0001] The invention relates to a method for preparing centella asiatica total saponins by using a macroporous adsorption resin. Background technique [0002] Centella asiatica (L.) Urban is a plant of the genus Centella asiatica in the Umbelliferae family. etc. It has a long history of application in the field of traditional medicine in many countries and regions. The internal and external use of Centella asiatica in traditional Chinese medicine in my country has a history of two thousand years. Centella asiatica is bitter in the mouth, pungent, cold in nature, nontoxic, returns liver, spleen, kidney, stomach meridian. According to ancient pharmacopoeia records, it is mainly used to treat diseases such as damp-heat jaundice, heatstroke diarrhea, sand and blood, carbuncle sores, and bruises. Modern pharmacological research and application have shown that Centella asiatica can be used to treat depression, skin wounds, gastric ulcers, infectious hepatitis, ski...

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Problems solved by technology

[0007] At present, there are some documents and patent reports on the process of preparing total saponins from Centella asiatica, but generally speaking, there are deficiencies such as cumbersome process steps, low product purity, and low quality control level.

In the prior art, the steps of preparing total saponins from Centella asiatica are relatively cumbersome or have high key technical requirements, it is difficult to realize industrial production or the production cost is too high, and the product yield is low or the product content is not high, resulting in high product prices

For example, the method disclosed in patent CN97117787.2 uses a large amount of halides and higher alcohols and other non-volatile organic solvents during preparation, which not only causes high production costs, but also causes certain pollution to the environment; in the method disclosed in patent CN200380109575.3, using Anion-exchange resin and cation-exchange resin are used, and the step of selective degreasing is adopted in the middle, and the process is complicated; in the method disclosed in patent CN01113014.8, two kinds of macroporous adsorption resins, D101 and ZTC-1, are used, but they are not effective for Centella asiatica. The adsorption amount of saponin is small, and the product recovery rate and content are not high

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