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Antitumaous echinus shell polysaccharide and preparation thereof

A technology for chitin and sea urchin, applied in the field of sea urchin chitin and its preparation, can solve the problems of high cost, complicated process, difficult mass production and the like, and achieve the effects of low cost, simple preparation process and wide source of raw materials

Inactive Publication Date: 2008-10-08
白日霞 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the technical problems of high cost, complex process, and difficulty in realizing large-scale production in the prior art, and provides a sea urchin chitosan with anticancer effect and low cost, simple in process, and capable of large-scale production. its preparation method

Method used

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  • Antitumaous echinus shell polysaccharide and preparation thereof
  • Antitumaous echinus shell polysaccharide and preparation thereof
  • Antitumaous echinus shell polysaccharide and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0014] a. Take horse dung sea urchin shells and extract them with water at 70-90°C for 2-4 hours;

[0015] b. concentrating and filtering the extract to obtain a filtrate;

[0016] c. Add absolute ethanol whose volume is 3.5 to 4.5 times that of the filtrate, centrifuge at a speed of 3000 to 5000 rpm, discard the supernatant, and obtain a precipitate;

[0017] d. Dissolve the precipitate in hot water, add methanol with a volume 0.5 to 1 times that of the solution, and filter to obtain the precipitate.

[0018] Dry the precipitate to obtain a milky white powder that is soluble in water but insoluble in alcohol, ether, etc., and is identified as a single component by high performance liquid chromatography and ultracentrifugation, with a molecular weight of 2.0×10 3 dalton. with methanolysis, gas chromatography, methylation, gas-mass spectrometry, periodate oxidation, Smith degradation, 1 H and 13 C nuclear magnetic resonance, atomic force electron microscopy and other method...

Embodiment 2

[0022] The steps of extracting sea urchin chitin are the same as in Example 1, but the step a is to extract 6 times with 90° C. water respectively, and combine the leaching solutions obtained from the 6 times.

[0023] Determination, inspection method and experimental data, optimum action amount are with embodiment 1.

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Abstract

The invention discloses a sea urchin shell polysaccharide extract which is extracted from sea urchin shell and has anticancer effect and also discloses the preparation method of the sea urchin chitosan. The invention is characterized in that 1,2 bond fucoidan sulfate which is water-soluble ivory powder, with the molecular structural formula as follows:. The preparation method has the following procedures: horse dung sea urchin shells are leached in the water at 70 to 90 DEG C for 2 to 4 hours; the extract liquor is concentrated and filtered to obtain the filtrate; anhydrous ethanol with the volume 3.5 to 4.5 times that of the filtrate is added into the filtrate and then centrifuged at a rotating speed of 3000 to 5000 r / m; then the supernatant is discarded and the sediment is obtained; the sediment is dissolved in hot water, then methanol with the volume 0.5 to 1 time that of the dissolved solution is added and filtered to get the sediment.

Description

Technical field: [0001] The invention relates to sea urchin chitosan extracted from sea urchin shells and having anticancer activity and a preparation method thereof. Background technique: [0002] Sea urchin is a kind of echinoderm, mainly including purple sea urchin, shrimp pancreas urchin and crown-of-thorns sea urchin. The nutritional and medicinal value of sea urchin is very high, and its edible part is the gonad (ovary, testis) in its body cavity, collectively referred to as sea urchin eggs, also known as sea urchin yellow or sea urchin paste, and sea urchin shells are usually discarded because they are inedible. At present, in the prior art, there is a record of extracting the anticancer substance-echinacea polysaccharide SEP from sea urchin yellow, but because the sea urchin yellow raw material is scarce, the cost is high, and the process of extraction requires multiple chromatographic column purifications, the process is complicated. The cost of echinacea polysacch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/737A61P35/00
Inventor 白日霞薛白
Owner 白日霞