Method for promoting topical drug release on bone cement carried with antibiotics

A local drug and bone cement technology, applied in medical science, prostheses, etc., can solve the problems of antibiotics that are never released, slow drug release, and prolonged infection, and achieve the prevention of adhesion and secretion, high biocompatibility, and sensitive recovery sexual effect

Inactive Publication Date: 2011-07-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, recent basic drug research shows that the release of antibiotic-loaded bone cement is slow and limited in the later stage, and most of the antibiotics embedded in the deep part of the bone cement will never be released.
Ultra-long-term sub-therapeutic concentrations of local antibiotics are likely to cause bacterial gene mutation resistance and selective drug resistance, and are ineffective against bacterial pellicles. In addition, their rough surfaces can further serve as carriers for bacterial adhesion and secretion to form bacterial pellicles. The above mechanism leads to persistent infection and even intractable

Method used

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  • Method for promoting topical drug release on bone cement carried with antibiotics
  • Method for promoting topical drug release on bone cement carried with antibiotics
  • Method for promoting topical drug release on bone cement carried with antibiotics

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: the embodiment of the present invention selects the low-frequency ultrasonic treatment instrument as Chongqing Jingpeng Electronics Co., Ltd., and the model is a DC20A ultrasonic transmitter. It has a housing. The front side of the housing has an LCD display screen, and the display screen has a touch screen input, The upper part of the front side has indicator lights and the lower part has a keyboard, and the back of the housing has power connection terminals, probe connectors and contrast knobs. The LCD display is connected with the processor and can display working parameters, status, and real-time clock. After the touch screen is connected to the touch screen controller, it is controlled by the processor. The real-time clock uses the functional parts integrated in the processor. The ultrasonic signal generator is realized by controlling the voltage-controlled oscillator by the analog voltage signal output by the D / A functional part integrated in the proces...

Embodiment 2

[0028] Example 2: Low-frequency ultrasound promotes drug release from antibiotic-loaded bone cement in vitro.

[0029] Put 40g of bone cement powder and 2g or 4g of vancomycin into an airtight container, stir magnetically for 24h (150r / min) to make them evenly mixed, add 18.88g of solidification liquid, stir well to form a dough, and pour into the prefabricated mold (top surface Square width 0.5cm, bottom surface square width 1.5cm, height 1.8cm) in the casting mold, solidify at room temperature and take out to be the hexahedral antibiotic bone cement with vancomycin mass fraction of 5%, 10%, and the weight is respectively (2.16± 0.03) g, (1.96±0.02) g, and the vancomycin contents were (83.08±1.27) mg and (160.42±4.20) mg, respectively. Place it under normal pressure, room temperature and ventilated environment for 24 hours, and disinfect it with disposable ethylene oxide (400mg / L) under normal pressure for 2 hours.

[0030] The specimens were randomly divided into three grou...

Embodiment 3

[0033] Example 3: Microbubble-enhanced low-frequency ultrasound promotes drug release from antibiotic-loaded bone cement under in vitro conditions.

[0034] The preparation method of Example 2 was used to prepare the disk-shaped specimen (diameter 10 mm, thickness 5 mm) loaded with antibiotic bone cement. The specimens were soaked in 10ml of sterile PBS at 37°C. The specimens were divided into 3 groups: control group, ultrasonic group (frequency 46kHz, on-off time ratio 40min:10min, sound intensity 300mW / cm 2 ), microbubble ultrasound group (Sonovue, mass fraction 0.025%, added locally). Keep the distance between the probe and the top surface of the specimen at 2 cm, and immerse the probe in PBS before ultrasonication. PBS was replaced every 24 hours, and the initial concentration of microbubbles and sound-conducting glue was supplemented. Within 14 days of immersion, 100ul was sampled daily and centrifuged (3000r / s, 5min). The concentration of vancomycin was determined by ...

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Abstract

The present invention provides a releasing method for promoting antibiotics loading bone cement local drug. The acrylic acid bone cement of loading antibiotics is put in a volume bottle containing phosphate buffer liquid. Micro bubble agent is injected. An ultrasonic probe is arranged at the bottom of the volume bottle. An ultrasonic generation device is started. The ultrasonic frequency is 20-100 KHz, and the space average time strength is 20mW/cm<2>-3W/cm<2>. The model includes a continuous type and a pulse type. The duty cycle is 20 to 100 percent. The modulation pulse frequency is 1Hz-10 KHz. The method can promote the releasing speed and the total amount of the antibiotics loading bone cement drug and maintain for a certain time, can effectively prevent the formation of pellicle, can enhance the sensitivity of bacteria to the antibiotics, can effectively prevent the adhesion of the bacteria and secrete the pellicle, thereby improving the antibacterial effect of the antibiotics loading bone cement and expanding the application range of the antibiotics loading bone cement.

Description

technical field [0001] The invention belongs to the drug release of antibiotic-loaded bone cement for artificial joints, in particular to a method for promoting the release of drugs in the antibiotic-loaded bone cement. Background technique [0002] Infection is one of the most common and serious complications after joint replacement surgery. Although in recent years, with the continuous development of aseptic technique, perioperative antibiotics and surgeons' aseptic concept, the incidence rate has dropped to 1%-5%, but the number of infection cases is still rising rapidly due to the continuous increase in the number of operations. Among the 500,000 cases of artificial joint replacement in the United States every year, the number of cases of infection is as high as 5,000-10,000, and the average treatment cost for each case is as high as 60,000 US dollars. Patients need to experience 6-18 months of physical and mental torture. Although there is no statistical data on large ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54
Inventor 严世贵蔡迅梓
Owner ZHEJIANG UNIV
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