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Method for rapidly testing cysteine in water solution

A technology of cysteine ​​and aqueous solution, which is applied in the field of cysteine ​​detection, can solve the problems of inability to be fully automated, time-consuming and laborious, and limit applications, and achieve the effects of low production cost, convenient use, high sensitivity and selectivity

Inactive Publication Date: 2008-12-24
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] 1. High-performance liquid chromatography and electrochemical assays require the help of complex instruments, and require pretreatment of specimens and cannot be fully automated. The operation is complicated, time-consuming and laborious, which limits their clinical application;
[0012] 2. Although enzyme immunoassay has good sensitivity and specificity, it is expensive because it is a biological preparation, which also limits its popularization and application;
[0013] 3. Other analysis methods have certain limitations because they need to be measured in organic or semi-aqueous solutions, which may easily cause secondary pollution.

Method used

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  • Method for rapidly testing cysteine in water solution
  • Method for rapidly testing cysteine in water solution
  • Method for rapidly testing cysteine in water solution

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1: The method for rapid detection of cysteine ​​in aqueous solution by spectrometry

[0051] Prepare HEPES (10mM) buffered aqueous solution with pH 6.0, and prepare 2×10 -3 M copper nitrate aqueous solution, 2×10 -3 M xylenol orange aqueous solution and 2 x 10 -3 Cysteine ​​aqueous solution of M; add 2mL of HEPES buffer solution to a clean UV cuvette as a blank, draw 2×10 -3 25 μl of xylenol orange solution of M was added to the cuvette, and the solution turned from colorless to yellow at this time, detected on a UV-visible spectrophotometer, and there was a maximum absorption at 441nm; in the above-mentioned cuvette, add 2 ×10 -3 25 μ l of copper nitrate solution of M, at this moment, the solution was changed from yellow to purple, and detected on the UV-visible spectrophotometer, it was found that the maximum absorption peak changed from the above-mentioned 441nm to 572nm, and the UV-visible absorption spectrum was shown in figure 1 Take cysteine ​​aque...

Embodiment 2

[0052] Embodiment 2: the method for the rapid detection of cysteine ​​in aqueous solution by solution colorimetry (kit method)

[0053] Kit preparation:

[0054] Prepare HEPES (10mM) buffered aqueous solution with pH 6.0, and prepare 2×10 -3 M copper nitrate aqueous solution, 2×10 -3 M xylenol orange aqueous solution and 2 x 10 -3 M cysteine ​​aqueous solution; take five colorimetric tubes, respectively add 2ml of HEPES buffer solution, 25μl 2×10 -3 The xylenol orange solution of M, at this time the solution turns from colorless to yellow, denoted as R 1 , R 2 , R 3 , R 4 , R 5 ; in R 2 , R 3 , R 4 , R 5 Add 2×10 -3 M copper nitrate solution 25μl, at this time the solution changed from yellow to purple, use a micro-sampler to absorb 35μl, 65μl, 100μl cysteine ​​and add to R 3 , R 4 , R 5 In, colorimetric visual view image 3 , R 3 , R 4 , R 5 The represented cysteine ​​concentrations are 35 μM, 65 μM, 100 μM, respectively.

[0055] Unknown concentration cys...

Embodiment 3

[0057] Embodiment 3: the method for rapidly detecting cysteine ​​in aqueous solution by test paper colorimetry

[0058] Prepare HEPES (10mM) buffered aqueous solution with pH 6.0, and prepare 2×10 -3 M copper nitrate aqueous solution, 2×10 -3 M xylenol orange aqueous solution and cysteine ​​aqueous solution with concentrations of 0.01, 0.04, 0.08, and 0.1 mM; 25 μl of Cu 2+ Solution and 25 μl of xylenol orange solution were added to 2ml of HEPES (10mM) buffer solution. After stirring evenly, the solution was purple-red. Immerse a large piece of filter paper in the purple-red solution for 10 seconds, take it out and dry it in the air, and then Repeated infiltration and drying for 3 times, the cysteine ​​test paper can be used, and the test paper is cut into strips for use;

[0059] Take 5 strips of strip test paper, soak them in the cysteine ​​aqueous solution with a concentration of 0, 0.01, 0.04, 0.08, and 0.1mM for 3 seconds, and take them out to obtain purple (blank), pur...

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Abstract

The invention provides a method for quickly detecting cysteine in a water solution. The method directly detects the content of the cysteine in a buffer solution (the pH value is 6.0) qualitatively and quantitatively by using xylenol orange as a developer on the basis of cupric ion Cu<2+>; the detection process is simple, the judging is objective and convenient and the method of the invention is easy to be generalized and applied. The method of the invention has exact measurement result and high sensitiveness, is not interfered by other aminophenol and negative ions, and can be generalized and applied to the determination of cysteine in clinical blood samples, urine samples and food.

Description

Technical field: [0001] The invention relates to cysteine ​​detection technology, in particular to a method for rapidly detecting cysteine ​​in aqueous solution, including a detection kit and a detection test paper. Background technique: [0002] Cysteine ​​(Cys for short) is an important sulfur compound amino acid, which is involved in various important cell functions, including protein synthesis, detoxification and metabolism; in the metabolism of organisms, cysteine ​​participates in the metabolism of cells The reduction process has the functions of regulating the metabolism of phospholipids in the liver and protecting liver cells from toxic damage. Disrupted cysteine ​​metabolism leads to cystinosis, a chromosomal recessive genetic disorder in which defects arise. The increase of cysteine ​​content in the body is implicated with hyperhomocysteinemia, which can cause some life-threatening physiological diseases, including Alzheimer's disease (senile dementia: Li Yingjuan...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/68G01N33/52
Inventor 霍方俊阴彩霞孙远强
Owner SHANXI UNIV
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