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Mulberry bark extract capable of reducing blood sugar and preparation method thereof

An extract and a hypoglycemic technology are applied to the mulberry bark extract with hypoglycemic activity and the field of preparation thereof, which can solve the problems of low yield, complicated process and the like, and achieve the effects of high yield, simple process and convenient packaging.

Inactive Publication Date: 2009-01-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the above-mentioned methods use more than ten traditional Chinese medicines and Mori twigs together to make a pharmaceutical composition, while the preparation of a single medicine is complicated or the yield is low due to the repeated use of column chromatography separation and purification.

Method used

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  • Mulberry bark extract capable of reducing blood sugar and preparation method thereof
  • Mulberry bark extract capable of reducing blood sugar and preparation method thereof
  • Mulberry bark extract capable of reducing blood sugar and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment one: the preparation of mulberry bark extract (1)

[0026] The mulberry bark is manually stripped from the fresh mulberry branches cut after the mulberry leaves are picked in the spring silkworm, summer silkworm, and autumn silkworm stages; Immerse in hot water for 1.0 h, then peel off the mulberry bark by hand or machine; the obtained mulberry bark is dried or dried and crushed by machine to make mulberry bark powder.

[0027] The mulberry bark powder was extracted with 30% ethanol aqueous solution (volume ratio) at 70° C. for 24 hours under reflux. The obtained alcohol-water extract is first filtered to remove water-insoluble matter and residue, and then concentrated by a rotary concentrator to obtain an alcohol-water concentrate. Add 2% papain, shake at 60°C for 2 hours, and then use a mixed solvent of chloroform and n-butanol (volume ratio 4:1) to repeatedly extract several times to remove enzymatic miscellaneous proteins (Sevag method). Then add 1% act...

Embodiment 2

[0029] Embodiment two: the preparation of mulberry bark extract (2)

[0030] The mulberry bark powder was extracted with 50% butanol aqueous solution (volume ratio) at 60° C. for 24 hours under reflux. The obtained alcohol-water extract is first filtered to remove water-insoluble matter and residue, and then concentrated by a rotary concentrator to obtain an alcohol-water concentrate. Add 2% papain, shake the enzymatic hydrolysis at 60°C for 2 hours, and then use a mixed solvent of chloroform and n-butanol (4:1) to repeatedly extract several times to remove the impurity protein of the enzymatic hydrolysis. Then add 1% activated carbon for decolorization, filter with suction to obtain a light yellow and clear active extract of mulberry bark, which is spray-dried or vacuum freeze-dried to make powdered mulberry bark extract (2).

Embodiment 3

[0031] Embodiment three: Polysaccharides, flavonoids, alkaloid content determination

[0032] The mulberry bark extracts (1) and (2) obtained in Examples one and two were determined.

[0033] 1. Polysaccharides

[0034] Determination of total sugar-phenol-sulfuric acid method: Accurately weigh 15mg of anhydrous glucose reference substance dried at 105°C to constant weight, put it in a 250ml measuring bottle, add water to dissolve and dilute to the mark, shake well, and obtain (each 1ml contains water glucose 0.06mg). Precisely measure 0.0, 0.2, 0.4, 0.6, 0.8, 1.0ml of the reference solution, put them in 10ml stoppered test tubes, add water to 1.0ml, add 1.0ml of 5% phenol solution, mix well, and quickly add 5.0ml of sulfuric acid, Shake well, put it in a boiling water bath for 5 minutes, take it out, cool it down, take the first part as a blank, and test it according to the spectrophotometry (Appendix VB, Part One of the Chinese Pharmacopoeia 2000 Edition), measure the absor...

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Abstract

The invention discloses a preparation method of a mulberry bark extract with blood glucose reducing effect, which comprises the steps of: peeling mulberry barks from mulberry twigs, drying, and pulverizing to obtain mulberry bark powder; extracting with 10-95% ethanol aqueous solution at 60-90 DEG C under reflux for 1-24 hours, filtering the obtained extractive solution to remove insoluble substances and residues, and concentrating with a rotary concentrating machine to obtain a concentrated solution; adding 2% papain, and allowing enzymolysis under shaking at 60 DEG C for 2 hours; removing proteins with a chloroform / n-butanol mixture solution; adding 1% activated carbon for decolorizing, filtering to obtain a straw yellow colored clear mulberry bark active extractive solution, and spraying drying or vacuum freeze drying to obtain a powdered mulberry bark extract. The inventive preparation method directly extracts a hypoglycemic drug with ALPHA-glycosidase inhibitor activity from the single medicinal raw material mulberry barks peeled from mulberry twigs; and has the advantages of simple process, low cost, and high efficiency.

Description

technical field [0001] The invention relates to an extraction and separation method using mulberry bark extract and its medical use as an inhibitor of alpha-glucosidase activity. Background technique [0002] Diabetes mellitus (Diabetes Mellitus) is a common endocrine metabolic disease caused by genetic and environmental factors. According to the statistics of the World Health Organization, there are currently more than 150 million diabetic patients in the world, and nearly 20 million diabetic patients in my country, which is expected to double to about 40 million by 2025. Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia caused by insufficient insulin secretion and / or impaired insulin action. It is manifested as an abnormal increase in the concentration of glucose in the blood and urine. When the blood sugar and urine sugar are too high, the typical symptoms of "three more and one less" will appear, that is, excessive drinking, polyuria, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P3/10A61K135/00
Inventor 张雨青马永雷周丽霞阎海波沈卫德
Owner SUZHOU UNIV
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