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Method for conversing agricultural and forest residues to be fermentable sugars

A technology of agricultural and forestry waste and sugar fermentation, applied in the field of cellulose enzymatic hydrolysis, can solve the problems of high cost of enzyme utilization, difficulty in removing lignin, waste of cellulase, etc., achieve convenient operation, reduce non-productive adsorption, and utilize high rate effect

Inactive Publication Date: 2009-01-07
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a large agricultural country with a very rich raw material of crop straws, which produces about 700 million tons of crop straws every year, including 230 million tons of rice straws, 220 million tons of corn straws, 120 million tons of wheat straws, and 100 million tons of bean and miscellaneous grain crop straws. tons, and another 100 million tons of straw such as peanuts, but the current utilization rate is very low, less than 20%.
The main problem is that the efficiency of enzymatic hydrolysis is often affected by the fiber structure of agricultural and forestry waste, the hydrolysis cycle is long, and the cost of enzyme utilization is high, which has become the biggest bottleneck in the industrialization of enzymatic hydrolysis
[0005] The important reason for the low yield of enzymatic hydrolysis is that natural fiber raw materials contain lignin, and it is difficult to completely and effectively remove lignin during pretreatment of agricultural and forestry wastes. Cellulase is wasted due to the plain surface, resulting in low yield of cellulase in the enzymatic hydrolysis process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of cellulase by fermentation of Trichoderma reesei.

[0030] 1. Formula of enzyme-producing culture medium: pulp 10g / L, glucose 1.0g / L, peptone 1.0g / L, potassium dihydrogen phosphate 2.0g / L, magnesium sulfate heptahydrate 0.05g / L, calcium chloride 0.4g / L L, adjust the pH to 5.0.

[0031] 2. Get 50mL of culture solution and place it in a 250mL conical flask with a cotton plug, inoculate Trichodermareesei QM9414 in the conical flask, cultivate it on a constant temperature oscillator at 170r / min, and control the enzyme temperature at (30 ±1)℃

[0032] 3. After culturing for 4 days, centrifuge at 3000r / min for 10min, take the supernatant as the crude enzyme solution, and measure the enzyme activity of the filter paper as 2.15FPIU / mL.

Embodiment 2

[0034] 1. Put 22g of crushed corn stalks into a steam explosion tank under the condition of 1.2MPa for 14 minutes, and then release the pressure instantly to obtain the corn stalks after steam explosion treatment, and wash them with water 10 times the dry weight of corn stalks 5 times to neutral, air dry for later use.

[0035] 2. After mixing the dried steam explosion slag with pH 4.8 citric acid / sodium citrate buffer, add cellulase (purchased from Sigma Company), the usage of cellulase is 10 FPIU / g corn stalk dry steam explosion The slag has a solid-to-liquid ratio of 1:9 (weight ratio), and an active agent PEG800 10g / L enzymolysis solution is added.

[0036] 3. Under the condition of 50°C, stirring condition: 100r / min, enzymolysis for 48h.

[0037] 4. The fermentable sugar yield obtained under the above conditions is: 89%.

Embodiment 3

[0044] 1. Put 24g of crushed wheat straw into a steam explosion tank under the condition of 1.6MPa for 7 minutes, and then release it instantly under reduced pressure to obtain the wheat straw after steam explosion treatment, and wash it with water 6 times the dry weight of wheat straw 4 times to wash with clean water until neutral, air dry for later use.

[0045] 2. After mixing the dried steam explosion slag with water, use 1% sulfuric acid and 1% NaOH to adjust the pH to 5.0, add cellulase (produced by Trichoderma reesei), and the usage of cellulase is 12 FPIU / g Dry steam explosion slag of wheat straw, the solid-to-liquid ratio is 1:9, and the active agent PEG80001g / L enzymatic hydrolysis solution is added.

[0046] 3. Under the condition of 40°C, stirring condition: 200r / min, enzymatic hydrolysis for 48h.

[0047]4. The fermentable sugar yield obtained under the above conditions is: 78%.

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Abstract

The invention belongs to the field of cellulase hydrolysis and discloses a method for converting forestry and agricultural residues into fermentability sugar. The method includes adopting steam explosion pre-treatment and washing on the forestry and agricultural residues after being cracked; then zymohydrolysis and saccharification is carried out. An activator is used during the zymohydrolysis and saccharification process. Compared with the prior method, as the activator is added to stop the lignin to generate non-productability absorption to the cellulose, the method of the invention improves the effective concentration of the cellulose and solves the problem of more consumption of the cellulose during the traditional hydrolyzing technique. The method of the invention has the characteristics of low cellulose consumption, simple technique, high sugar conversion rate, etc.

Description

technical field [0001] The invention belongs to the field of cellulose enzymatic hydrolysis, and relates to a method for converting agricultural and forestry wastes into fermentable sugars, which improves the sugar yield by converting cellulose. technical background [0002] With the rapid development of industry and the advancement of science and technology, the depletion of non-renewable resources and the gradual improvement of environmental protection standards, it is urgent to develop new and clean alternative energy sources. Agricultural and forestry waste raw materials are a very large amount of renewable resources in nature, and also a huge potential resource for ethanol production, which has great potential for solving energy problems. my country is a large agricultural country with a very rich raw material of crop straws, which produces about 700 million tons of crop straws every year, including 230 million tons of rice straws, 220 million tons of corn straws, 120 m...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12N9/42
Inventor 欧阳嘉李鑫陈牧宋向阳徐勇勇强
Owner NANJING FORESTRY UNIV
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