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A biomass raw material, pretreatment technology, applied in the direction of biofuel, fermentation, etc., can solve the problems of complex by-products, high cost and high requirements, and achieve the effect of being conducive to hydrolysis
Inactive Publication Date: 2009-01-14
SHANGHAI JIAO TONG UNIV
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The acid method can break the hydrogen bond between lignin-cellulose-hemicellulose and hydrolyze hemicellulose into monosaccharides, but it will also degrade lignin into "toxic substances". The by-products are complex and have great impact on subsequent fermentation Strong inhibitory effect; Alkaline method can remove lignin, but the wastewater pollution is serious; Ammonia fiber blasting method, steam explosion method, etc. have high requirements for equipment and high cost
[0003] Find through the retrieval of prior art document, recent bibliographical report acetone phosphate resolution method can separate lignin, cellulose and hemicellulose (Zhang Y-HP, Ding SY, Jonathan RM, Biotechnol Bioeng, 2007,97 (2): 214-223), the sugar recovery rate obtained by using the acetone phosphate resolution method is 96%, and does not produce inhibitory effect, but the equipment cost needed in the concentrated acid environment is expensive, and the pollution to the environment is larger
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Embodiment 1
[0030] Embodiment 1: Determination of samples
[0031] In this example, corn stalks purchased from the State Key Laboratory of East China University of Science and Technology were used. Divide the whole corn stalk into six parts: stalk, corn, leaf, tassel, husk, and whisker, and the proportion of each part is, for example, figure 1 shown. Among them, straw and leaves account for the largest proportion, so this embodiment uses leaves and straw as materials for processing and comparison.
Embodiment 2
[0032] Embodiment 2: Pulverization of samples
[0033] The leaves and stalks were pulverized with a pulverizer, passed through 20mm, 7mm, 0.9mm, 0.2mm, 0.074mm, 0.04mm sieves, and some samples passed through a 0.9mm sieve were pulverized with an ultrasonic airflow nano pulverizer, and four gradients of 8 A sample: 7-20mm (L 7-20mm , S 7-20mm ), 0.2-0.9mm (L 0.2-0.9 , S 0.2-0.9 ), 40-70μm (L 40-70μm , S 40-70μm ), the sample 10-20μm (L 10-20μm , S 10-20μm ).
Embodiment 3
[0034] Embodiment 3: the analysis of sample particle size distribution
[0035] Wherein the sample range is 40-70 μ m and the sample after the nano pulverizer is pulverized (purchased in Shanghai Huali Sofi Technology Co., Ltd.), the particle size range is 10-20 μ m, and the particle size distribution is measured with a laser particle size analyzer, and the measurement results are as follows: figure 2 shown.
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Abstract
Disclosed is a pretreatment method for biomass material, which comprises the steps: by adopting a nanometer grinding device, corn leaves and straw stalks are ground with the size rang between 10 micrometres to 20 micrometres, so the cellulose crystal thereof is exposed on the surface to be directly hydrolyzed with a hydrolysis efficiency of 96 percent which is identical to that of the sample split through the splitting method of phosphoric acid acetone. The pretreatment method can replace other pretreatment methods requiring chemical reagent, both the cost and the environmental pollution are reduced, and the pretreatment method is a promising raw material pretreatment technique which utilizes lignocellulose to produce biological energy sources or useful chemicals.
Description
technical field [0001] The invention relates to a pretreatment method in the field of biological clean energy, in particular to a pretreatment method for biomass raw materials. Background technique [0002] At present, microorganisms that can directly decompose lignin and hemicellulose act very slowly, and the cellulose in biomass raw materials cannot be effectively utilized. Cellulase can not directly act on stalks, can not be directly hydrolyzed, and needs physical and chemical methods to separate and split or directly degrade lignin and hemicellulose (Lloyd TA, Wyman CE, Biores Technol, 2005 (96): 1967- 1977; Teymouri F, Laureano-PerezL, Alizadeh H, Dale BE, Biores Technol, 2005(96):2014-2018; Kim S, HoltzappleMT, Biores Technol, 2005(96):1994-2006; Pan XJ, Neil G. , John K, BiotechnolBioeng, 2006, 94 (5): 851-861), including acid method, alkali method, organic solvent, high temperature and high pressure and other means to pretreatment, which is the current method for th...
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