Multi-nutrilite composite yeast powder
A compound yeast and multi-nutritional technology, applied in food preparation, application, food science, etc., can solve the problems of large side effects and poor effects, achieve high content, improve malnutrition, and benefit the effect of human nutrition balance
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[0037] In one embodiment of the present invention, a method for preparing high-protein yeast is provided, which includes the following steps:
[0038] (1) High-protein yeast seed culture: inoculate the high-protein yeast culture solution into 0.5-15L of sugar (wort or molasses or mixture of wort and molasses) of 25-30%, ammonia or urea In a seed fermenter with a content of 16%, a dihydrogen phosphate content of 10%, and a pH value of 4.6 to 5.0, culture at 28 to 30°C for 24 to 48 hours. Among them, the sugar source, nitrogen source and phosphorus source are added in a stream-feeding manner.
[0039] (2) Preparation of high-protein yeast: inoculate the seed culture solution to 1 to 300 tons of sugar (starch hydrolyzed sugar or molasses or a mixture of starch hydrolyzed sugar and molasses) of 25-30%, and ammonia or urea content of 16% In a fermenter with a 10% ammonium dihydrogen phosphate content and a pH of 4.8-6.5, the nutrient source used is fed by a feeding method to control th...
Embodiment 1
[0057] Example 1: Preparation of high-protein yeast
[0058] (1) High-protein yeast seed culture: Inoculate the high-protein yeast culture solution into 5L of sugar (wort or molasses or a mixture of wort and molasses) of 25%, and ammonia or urea content of 16% , Ammonia phosphate dihydrogen phosphate content of 10%, pH 4.8 seed fermentation tank, at 30 ℃, culture for 48 hours. Among them, the sugar source, nitrogen source and phosphorus source are added in a stream-feeding manner.
[0059] (2) Preparation of high-protein yeast: inoculate the seed culture solution to 1 ton of sugar (starch hydrolyzed sugar or molasses or a mixture of starch hydrolyzed sugar and molasses) of 25%, ammonia or urea content of 16%, dihydrogen phosphate In the fermenter with 10% ammonium content and pH 4.8, the nutrient source used is fed by flow to control the concentration of ammonia or urea in the fermentation broth, cultured at 30°C for 15 hours, where the sugar source, nitrogen source and phosphor...
Embodiment 2
[0060] Example 2: Preparation of chromium-rich yeast
[0061] (1) Chromium-rich yeast seed culture: Inoculate the chromium-rich yeast culture solution into 10L of sugar (wort or molasses or a mixture of wort and molasses) with an amount of 20%, chromium (chromium trichloride) The concentration is 100μg / ml, supplemented with appropriate nitrogen and phosphorus sources, and a seed fermenter with a pH of 5.5, cultured at 32°C for 36 hours.
[0062] (2) Preparation of chromium-rich yeast: inoculate the seed culture solution to 1 ton of sugar (starch hydrolyzed sugar or molasses or a mixture of starch hydrolyzed sugar and molasses) of 20%, and chromium (chromium trichloride) concentration of 100μg / ml, supplemented with appropriate nitrogen and phosphorus sources, in a fermenter with a pH of 5.5, the nutrient source used is fed by flow, and chromium is added in batches. After incubating at 32°C for 24 hours, centrifugal separation to remove the liquid phase , Wash the solid phase wit...
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