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Artificial culture method for zinc-rich Chinese caterpillar fungus fruiting body and culture medium thereof

A culture method and a culture medium technology, applied in the field of pharmacognosy, can solve the problems such as the lack of corresponding reports on the zinc-rich Cordyceps militaris culture method, and achieve the effects of significant immune regulation, low cost and high yield.

Inactive Publication Date: 2010-09-29
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no corresponding report on the zinc-rich Cordyceps militaris fruiting body products and their cultivation methods at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of strains:

[0035] Low temperature preservation of Cordyceps militaris species: Cordyceps militaris species Cordyceps militaris 5.701, from the China Common Microbial Species Collection Management Center.

[0036] Cordyceps militaris liquid strain: Cultivate according to the following process steps:

[0037] (1) Bacteria culture (activation) on the slope of test tube

[0038] Preparation of culture medium: 10g glucose, 10g peptone, 10g yeast extract, 10g agar powder, 960g water, with a pH of 5.0, and put them in test tubes; connect the cryopreserved strains to the slope of the test tube under aseptic conditions, 15 Incubate in the dark at ~30°C for 5-10 days.

[0039] (2) Triangular flask liquid seed culture:

[0040] Preparation of culture medium: 10g glucose, 10g peptone, 10g yeast extract, 970g water, and its pH value is 5.0. Divide the prepared medium into 500ml conical flasks, 200ml per bottle, and seal at 120~130℃ Sterilize for 18-30 minutes under the con...

Embodiment 2

[0054] (1) Preparation of strains:

[0055] Cordyceps militaris species: Cordyceps militaris 5.701, from the China Common Microbial Strain Collection Management Center.

[0056] (2) Preparation of zinc-rich medium:

[0057] The formula components are as follows by weight: 65g rice, 2.0g yeast extract, 85g water, 1g zinc sulfate, natural pH;

[0058] (3) Subpackage and sterilize the culture medium:

[0059] Dispense the prepared medium into 500ml canned bottles, seal it with a special sealing film for edible fungi, and sterilize it at 120-130℃ for 18-30 minutes;

[0060] (4) Cooling and inoculation:

[0061] After sterilization, take out the culture medium, cool it naturally to 20°C-30°C, and insert the solid cultured Cordyceps militaris strain under aseptic conditions;

[0062] (5) Training:

[0063] Place the inoculated zinc-rich Cordyceps militaris medium in a constant temperature incubator, culture at 20-30°C for 8-12 days. After the hyphae grow fully, transfer to 18-20°C, relative humid...

Embodiment 3

[0069] (1) Preparation of strains:

[0070] Cordyceps militaris species: Cordyceps militaris 5.701, from the China Common Microbial Strain Collection Management Center.

[0071] Cordyceps militaris liquid strain: Cultivate according to the following process steps:

[0072] (1) Bacteria culture (activation) on the slope of test tube

[0073] Preparation of culture medium: 20g glucose, 6g peptone, 9g yeast extract, 15g agar powder, 950g water, with a pH of 6.0, aliquoted into the test tube; connect the cryopreserved strains to the test tube slope under aseptic conditions, 15 Incubate in the dark at ~30°C for 5-10 days.

[0074] (2) Triangular flask liquid seed culture:

[0075] Preparation of culture medium: 20g glucose, 6g peptone, 9g yeast extract, 965g water, and its pH value is 6.0. Divide the prepared medium into 500ml conical flasks, 200ml per bottle, and seal at 120~130℃ Sterilize for 18-30 minutes under the conditions, remove the culture medium, and cool naturally to 20-30℃; unde...

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Abstract

The invention discloses an artificial culture method of a zinc-rich cordyceps militaris fruit body and a culture medium thereof. The culture medium comprises the following components by weight portions: 40-65 portions of rice, 0.5-2.0 portions of a yeast extract, 60-85 portions of water and 0.01-1.0 portion of zinc sulfate with the natural PH value. The artificial culture method comprises the following steps: preparation of the culture medium, canning and sterilization; cooling and inoculation; culture; collection of the fruit body; drying and granulation. The method has the advantages of simple operation, short culture period, high yield of the fruit body, no pollution, no waste and zinc supplement function, and the method is suitable for industrialized production.

Description

Technical field: [0001] The invention relates to an artificial culture method for zinc-rich Cordyceps militaris fruiting bodies and a culture medium thereof, and belongs to the technical field of pharmacognosy. Background technique: [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the genus Cordyceps of the Ascomycota, Sclerotomycetes, Ergotidae, and is one of the rare Chinese medicinal materials in my country. Modern pharmaceutical research shows that Cordyceps militaris contains nucleosides, polysaccharides, cordycepin, sterols and other ingredients basically the same as Cordyceps sinensis. It has therapeutic effects on immune system, nervous system and cardiovascular system diseases, and has anti-tumor and Anti-cancer effect, it is an ideal substitute for Cordyceps sinensis and has broad development prospects. [0003] Zinc is a kind of trace element. The content in the human body and the daily intake are very small, but it can play a pivotal role in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/00C12R1/645
Inventor 程显好盖宇鹏孙慧涌朱林丁娟冯志彬刘林德
Owner LUDONG UNIVERSITY
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