Method for separating and purifying glutamine and aminoglutaric acid from glutamine fermentation liquor

A glutamine and fermentation broth technology, applied in the field of simulated moving bed chromatographic separation, can solve problems such as inability to separate glutamine, low yield of glutamine and glutamic acid products, affecting product quality, etc., and achieve production cost Low cost, small production site and low labor intensity

Inactive Publication Date: 2009-03-04
WUXI GREEN SEPARATION TECH INST
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the separation and purification method is used in industry to remove impurities from glutamine fermentation broth, but this method cannot separate glutamine, glutamic acid from a large amount of inorganic salt and residual sugar impurities, which seriously affects product quality. Low yields of glutamine and glutamic acid products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and purifying glutamine and aminoglutaric acid from glutamine fermentation liquor
  • Method for separating and purifying glutamine and aminoglutaric acid from glutamine fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: synthetic resin

[0035]First synthesize two kinds of special resins (metal ion type chelating resin and acid radical ion type chelating resin) specially used for adsorption and separation of glutamine;

[0036] Metal ion-type chelating resins use acidic macroporous or gel resin as a carrier to chelate metal ions, use the affinity between the acidic resin and metal ions to adsorb the metal ions on the surface of the acidic macroporous resin, and use the A linking agent is used to fix it on the matrix resin; the amount of the crosslinking agent used is 4% to 20% of the weight of the acidic macroporous resin, and the best is 10% to 14%.

[0037] Metal ion is Al 3+ or Ba 2+ or Bi 3+ or Ca 2+ or Co 2+ or Co 3+ or Cu 2+ or Fe 2+ or Fe 3+ or K + or Mg 2+ or Na + or Ni 3+ or Pb 2+ or Sb 3+ or Sn 2+ or Zn 2+ , which is used in the form of a salt or oxide of a corresponding metal ion that can be dissolved under neutral or alkaline conditions, and t...

Embodiment 2

[0052] Embodiment 2: separation and purification

[0053] The fixed bed equipped with the above-mentioned special resins uses the simulated moving bed technology to separate and purify glutamine from the fermentation broth to obtain pure glutamine and glutamic acid products;

[0054] Simulated moving bed technology: In the simulated moving bed chromatographic separation device, the above-mentioned special resin is used as the stationary phase adsorbent, and the difference in affinity between the functional group and glutamine, glutamic acid and other impurities is used to realize glutamine, glutamic acid Complete separation from impurities such as inorganic salts and residual sugar; water is used as the eluent, the separation temperature is 35°C-95°C, and the optimum separation temperature is 50°C, and the feeding and discharging operations are carried out continuously to obtain three Class discharge components, the first category is the components with a glutamine purity of 8...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for separating and purifying glutamine and glutamic acid from glutamine fermenting liquor belongs to the technical field of simulation moving bed chromatographic separation. The method comprises the following steps: synthesizing two special resins special for separating glutamine by absorption: metal ion type chelating resin or acid ion type chelating resin; and separating and purifying the glutamine fermenting liquor using a fixed bed chromatographic separating column filled with one special resin by the simulation moving bed technology to acquire pure glutamine and glutamic acid product. The simulation moving bed technology is employed to thoroughly separate glutamine and glutamic acid from impurities such as inorganic salt and residual sugar. Only 1 to 2 m<3> of water and small amount of power are only required to separate every cubic glutamine fermenting liquor. The method has the advantages of high resin utilization rate, full automatic production process, low labor intensity, small production spot, low production cost, no requirement for chemicals and no pollution during the production process.

Description

technical field [0001] The present invention relates to the preparation method of high-purity glutamine and glutamic acid, in detail, it relates to the method for separating and preparing high-purity glutamine and glutamic acid from glutamine fermentation liquid by skillfully using special resin and simulated moving bed and craft. The invention belongs to the technical field of simulated moving bed chromatographic separation. Background technique [0002] The main production method of glutamine is to use glucose as the nutrient substance to first generate glutamic acid and then convert it into glutamine under the fermentation of corresponding enzymes and bacteria. Therefore, in addition to glutamine, there is a large amount of unconverted glutamic acid in the glutamine fermentation broth, as well as impurities such as residual sugar and inorganic salts composed of reducing sugar, polysaccharide, non-reducing sugar and other raw materials that have not been fermented. [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C237/06C07C231/22C07C229/24C07C227/40B01D15/36
Inventor 俞铮彭奇均
Owner WUXI GREEN SEPARATION TECH INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap