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Extraction technique of high-purity phlorizin

An extraction process and a technology for phloridzin, which are applied in the technical field of phloridzin extraction, can solve the problems of low content, high cost, residues and the like, and achieve the effects of simple extraction process, less residue and reducing organic residues

Inactive Publication Date: 2009-03-25
XINGHUA GL STEVIA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a new extraction process in order to overcome the problems of complex existing process flow, high cost, residue, and low content. This process not only simplifies the process flow, reduces production costs, but also reduces the organic Residue, improve product quality, high product content, up to 99%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A process for extracting high-purity phlorizin, comprising the following steps: (1) adding 8 times pure water to the apple juice waste with a water content of 3%, soaking for 3 hours, heating to boiling, and maintaining slight boiling for 2 hours, Obtain the extract; the residue is heated to boiling with 6 times pure water, and kept at a slight boil for 2 hours to obtain a secondary extract, which is combined, cooled, filtered, and the filtrate is collected; (2) the filtrate is absorbed by the macroporous resin D101 , the column flow rate is 80L / H, eluted with ethanol gradients of 20° and 50°, the effluent is collected in sections, concentrated in vacuum, left to stand, cooled, crystallized at 0-4°C, and dehydrated by high-speed centrifugation; (3) Redissolution in pure water, vacuum concentration, recrystallization, high-speed centrifugal dehydration, drying to obtain the finished product.

Embodiment 2

[0018] A process for extracting high-purity phlorizin, comprising the following steps: (1) adding 7 times pure water to apple juice waste with a water content of 1%, soaking for 2 hours, heating to boiling, and maintaining a slight boil for 3 hours, Obtain the extract; The residue is heated to boiling with 5 times of pure water, and kept slightly boiling for 2 hours to obtain the secondary extract, which is combined, cooled, filtered, and the filtrate is collected; (2) the filtrate is passed through the macroporous resin AB- 8 Adsorption, the flow rate through the column is 85L / H, eluted with ethanol gradient at 20° and 50°, the effluent is collected in sections, concentrated in vacuum, left to stand, cooled, crystallized at 0-4°C, and dehydrated by high-speed centrifugation; ( 3) Redissolving in pure water, vacuum concentration, recrystallization, high-speed centrifugal dehydration, drying to obtain the finished product.

Embodiment 3

[0020] A process for extracting high-purity phlorizin, comprising the following steps: (1) adding 4 times pure water to pear juice wastes with a water content of 3%, soaking for 1 hour, heating to boiling, and keeping slightly boiling for 3 hours , to obtain the extract; the residue was heated to boiling with 6 times of pure water, and kept slightly boiling for 2 hours to obtain a secondary extract, which was combined, cooled, filtered, and the filtrate was collected; (2) the filtrate was passed through the macroporous resin ADS -7 adsorption, the flow rate of the column is 90L / H, and the ethanol gradient of 20° and 50° is used for elution, the effluent is collected in sections, concentrated in vacuum, left to stand, cooled, crystallized at 0-4°C, and dehydrated by high-speed centrifugation; (3) Redissolve in pure water, concentrate in vacuum, recrystallize, dehydrate by high-speed centrifugation, and dry to obtain the finished product.

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Abstract

The invention discloses an extraction process of a high purity phlorizin, comprising the following steps: (1) the raw materials are added with 4-8 times pure water, marinated for 1-3 hours, heated to boiling and micro boiling is kept for 2-3 hours so as to obtain an extracting solution; residue is heated to boiling by using 2-6 times pure water and micro boiling is kept for 2-3 hours so as to obtain a secondary extracting solution; the extracting solutions are merged, cooled and filtered and then the filtered liquid is collected; (2) the filtered liquid is adsorbed by macroporous resin and subjected to gradient elution by using 20 DEG and 50 DEG ethanol; the effluent liquid is collected in subsection, concentrated in vacuum, placed in a quiescence way and cooled, dissolves out crystal at 0-4 DEG C, and dehydrates by high-speed centrifugation; and (3) re-dissolving of pure water, vacuum concentration, re-crystallization, high-speed centrifugation dehydration and drying are carried out so as to obtain the finished product. By adopting pure water for extraction, the organic-residue is decreased and simultaneously the product content is more than 99 percent.

Description

technical field [0001] The invention relates to a process for extracting active ingredients of natural plants, in particular to a process for extracting phlorizin from bark, roots, branches and leaves and juice waste of Rosaceae plants. Background technique [0002] Phlorizin belongs to plant polyphenolic compounds. There are many studies abroad on the physiological functions of phloridizin. Phlorizin can activate cellular protein kinase, inhibit cell proliferation, is an anti-mutagenic factor, and can be used for adjuvant therapy of skin liver cancer and other tumors. Correlation of phlorizin content (Shinohara1998) research shows that phlorizin can effectively slow down the proliferation and growth of colon cancer cells and liver tumor cells; it has strong antioxidant activity, and the antioxidant concentration of oil is between 10-30ppm ;Phlorizin has significant antihypertensive and lipid-lowering effects, and is known as a "natural antihypertensive drug", which is esp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C01G3/00A61K36/73A61K125/00
Inventor 周伯雅徐进权
Owner XINGHUA GL STEVIA CO LTD
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