Method for producing chitin, calcium citrate and protein powder

A technology of calcium citrate and chitin, which is applied in the field of producing chitin, calcium citrate and protein powder, can solve the problems of high loss of chitin, cumbersome process, pollution of environment production cost by acid-base waste liquid, etc. Reasonable process, good quality effect

Inactive Publication Date: 2009-04-15
RONGCHENG TAIXIANG FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiencies in the prior art that there are many losses of chitin, pollution of the environment by acid-base waste liquid, high production cost and cumbersome process, and the present invention provides a method for producing chitin, calcium citrate and protein powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Put 5kg of shrimp shells in an oven at 140°C to dry the raw materials, and keep it warm for 20 minutes, take out and add water 6 times the mass, heat to 55°C, add neutral papain with a mass ratio of 1:0.001 to shrimp and crab shells, At an enzymatic hydrolysis temperature of 55°C, stir and hydrolyze for 14 hours to obtain an enzymatic hydrolyzate, and boil for 5 minutes to inactivate the enzyme. Filtrate the enzymatic solution to obtain shrimp shells, add 20% citric acid solution and soak for 35 hours, then filter, wash with acid, and dry to obtain chitin. After filtration, the solution was placed in an environment of 15°C for 30 minutes, and the cold precipitated calcium citrate was removed by centrifugation, and the remaining liquid was protein liquid, which was concentrated and dried in a vacuum to obtain protein powder.

Embodiment 2

[0016] Put 10kg of crab shells into an oven at 120°C to dry the raw materials, and keep it warm for 50 minutes, take out and add water twice the mass, heat to 50°C, add neutral papain with a mass ratio of 1:0.002 to shrimp and crab shells, At an enzymatic hydrolysis temperature of 50°C, stir and hydrolyze for 10 hours to obtain an enzymatic hydrolyzate, and boil for 3 minutes to inactivate the enzyme. Filtrate the enzymatic solution to obtain shrimp shells, add 15% citric acid solution and soak for 24 hours, then filter, wash with acid, and dry to obtain chitin. After filtration, the solution was placed in an environment of 10°C for 50 minutes, and the cold precipitated calcium citrate was removed by centrifugation, and the remaining liquid was protein liquid, which was concentrated and dried in a vacuum to obtain protein powder.

Embodiment 3

[0018] Put 50kg of shrimp and crab shells in an oven at 130°C to dry the raw materials, and keep warm for 30 minutes, take out and add water 4 times the mass, heat to 52.5°C, add neutral papaya with a mass ratio of 1:0.003 to shrimp and crab shells Protease, at the enzymatic hydrolysis temperature of 52.5°C, stirred and hydrolyzed for 12 hours to obtain an enzymatic hydrolyzate, boiled for 4 minutes to inactivate the enzyme. Filtrate the enzymatic solution to obtain shrimp shells, add 17% citric acid solution and soak for 30 hours, then filter, wash with acid, and dry to obtain chitin. After filtering, the solution was placed in an environment of 12.5°C for 40 minutes, and the cold precipitated calcium citrate was removed by centrifugation. The remaining liquid was protein liquid, which was concentrated and dried in vacuo to obtain protein powder.

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Abstract

The invention relates to a method for producing chitin, calcium citrate and albumen powder by using the shells of crustaceans like shrimps and crabs as the materials; the shells of shrimps and crabs are selected and arranged into an oven, and dried and then water with weight of 6 times of the shells are added; then the mixture is heated to be between 50 and 55 DEG C; then a neutral papain with a mass ratio of 1: 0.001 to 0.003 with the shells of shrimps and crabs, is added for carrying out zymohydrolysis and obtaining zymohydrolysis liquid; the zymohydrolysis liquid is filtered for obtaining the shells of shrimps and crabs; 15% to 20% citric acid solution is added for dipping for 24 to 36 hours; then the chitin is obtained through filtering, acid rinsing and drying; cold separation is carried out on the filtered liquid and a cold separation matter which can be named the calcium citrate is obtained through centrifugalizing; vacuum condensing and drying are carried out on the rest liquid obtained through centrifugalizing, thus obtaining the albumen powder. In the invention, the technical method adopts enzymes for removing proteins and citric acid decalcification for leading the separation of the protein and the calcium with the chitin to be more complete; the yield rate of the chitin is high; and the prepared chitin product has the advantages of white color, high purity and good quality.

Description

technical field [0001] The invention relates to a production method of crustacean products, in particular to a method for producing chitin, calcium citrate and protein powder using shells of crustaceans such as shrimps and crabs as raw materials. Background technique [0002] In nature, chitin exists in the cells of lower plant fungi and algae, or in the shells of crustacean shrimp, crabs, insects, and cell walls of higher plants. Its content is not lower than that of rich cellulose. A large class of important polysaccharides. The quality of chitin varies greatly due to different raw material sources and process conditions. [0003] At present, the following two processes are generally used for chitin extraction: one is to deproteinize and fat the shrimp and crab shells with NaoH, and decalcify them with dilute acid in two steps. For example, the disclosed CN1085564A Chinese invention patent application publication discloses "a preparation method of chitin". The method is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P9/00C12P21/06
Inventor 李银塔刘扬瑞步营于玲
Owner RONGCHENG TAIXIANG FOOD
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