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Method for extracting lactoflavin

A technology of riboflavin and fermented liquid, which is applied in the fields of metabolic diseases, organic chemistry, fermentation, etc., can solve the problems affecting the separation of bacteria and product quality, low product purity, and rapid degradation of riboflavin, etc., and achieve less sewage discharge , The product has good color and less pigment

Inactive Publication Date: 2009-04-22
山东恩贝生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current operation process of the alkali dissolution method is complicated, the steps are long, the product purity is not high, and riboflavin is degraded quickly under alkaline conditions, and the yield is low, and a large amount of bacterial protein will be released into the solution under the operation conditions of the alkali dissolution method , affecting bacterial cell isolation and product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting riboflavin from riboflavin fermentation broth, the specific steps are:

[0020] (1) Add 500L riboflavin fermentation broth to a static mixer, add liquid caustic soda to adjust the pH to 8, adjust and control the pH by controlling the flow ratio of the fermented liquor and liquid caustic soda, so that the riboflavin is completely dissolved, and the mixing process is instantaneously controlled at 10 Seconds, the temperature is controlled at 20°C, and stirred for 5Min;

[0021] (2) Adopt 50nm microfiltration ceramic membrane to filter and remove bacteria, and adopt continuous online filtration mode, continuous filtration, continuous diafiltration, so that the residence time of materials in the system is 5 minutes, and the moisture solid of bacteria residue is controlled at 60%.

[0022] (3) XKDa roll-type organic membrane is adopted, and the filtration pressure is controlled to 10 bar to remove protein, pigment, etc.

[0023] (4) The filtrate is ox...

Embodiment 2

[0027] A method for extracting riboflavin from riboflavin fermentation broth, the specific steps are:

[0028] (1) Add 500L riboflavin fermentation broth to a static mixer, add liquid caustic soda to adjust the pH to 10, adjust and control the pH by controlling the flow ratio of the fermented broth and liquid caustic so that the riboflavin is completely dissolved. The mixing process was controlled instantaneously for 15 seconds, the temperature was controlled at 30°C, and the stirring was carried out for 8 minutes.

[0029] (2) Adopt 50nm microfiltration ceramic membrane to filter and remove bacteria, and adopt continuous online filtration mode, continuous filtration, continuous diafiltration, so that the residence time of materials in the system is 10 minutes, and the moisture solid of bacteria residue is controlled at 65%.

[0030] (3) XKDa roll-type organic membrane is adopted, and the filtration pressure is controlled to 15 bar to remove protein, pigment, etc.

[0031] (4...

Embodiment 3

[0035] A method for extracting riboflavin from riboflavin fermentation broth, the specific steps are:

[0036] (1) Add 500L riboflavin fermentation broth to a static mixer, add liquid caustic soda to adjust the pH to 13, and adjust and control the pH by controlling the flow ratio of the fermented broth and liquid caustic so that the riboflavin is completely dissolved. The instantaneous control of the mixing process only takes 20 seconds, the temperature is controlled at 40°C, and the stirring time is 10 minutes.

[0037] (2) Adopt 50nm microfiltration ceramic membrane to filter and remove bacteria, and adopt continuous online filtration mode, continuous filtration, continuous diafiltration, so that the residence time of materials in the system is 15 minutes, and the moisture and solidity of bacterial residue is controlled at 70%.

[0038] (3) XKDa roll-type organic membrane is adopted, and the filtration pressure is controlled to 20 bar to remove protein, pigment, etc.

[003...

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Abstract

The invention relates to a method for extracting riboflavin, in particular to the method for extracting the riboflavin from a riboflavin fermentation broth. The invention provides the method for extracting the riboflavin from the riboflavin fermentation broth to reduce the steps for extracting the riboflavin, reduce the production cost and achieve clean production. The method has the advantages of online control of the production process, short basic time, less degradation of the riboflavin, no acid-soluble back dissolving process, no generation of waste acid, less wastewater discharge, less equipment corrosion, mother liquor reclamation, pressure reduction on sewage treatment, product recycling, and improved yield by about 5 percent.

Description

technical field [0001] The invention relates to a method for extracting riboflavin, in particular to a method for extracting riboflavin from riboflavin fermentation liquid. Background technique [0002] Riboflavin is also known as vitamin B2, vitamin G or lactoflavin. The chemical name is: 7,8-dimethyl-10-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-3,10-dihydrobenzopteridine-2, 4-diketone. [0003] Riboflavin is yellow to yellow-orange crystal or crystalline powder, with slight odor and slightly bitter taste. The melting point is about 280°C (with decomposition). It is not affected by diffuse light when drying, and the solution is easily deteriorated when exposed to light. Its saturated solution is neutral to litmus. 1g can be dissolved in 3000-20000mL of water, depending on the crystal form. It is almost insoluble in ethanol, ether and chloroform, and has a high solubility in dilute alkaline solution, but it will deteriorate faster in alkaline solution or when exposed to l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D475/14C12P25/00A61P3/02
Inventor 左克峰朱翠云孙海远何永丰梁建勇
Owner 山东恩贝生物工程有限公司
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