Method for synthesizing biodiesel by two-step catalysis of lipid via Yarrowia lipolytica lipase, and biodiesel obtained thereby
A biodiesel and yeast technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increasing the risk of industrialized production, increasing the intensity of equipment investment, and the impact of production efficiency, so as to increase the feasibility of industrialization. performance, improved production efficiency, and extended service life
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0060] Preparation of crude lipase enzyme solution: Yarrowia lipolytica CGMCCNO.2707 was cultured on the slope of eggplant bottle for 12 hours and then inoculated in a fermenter for culture. After 90 hours of culture, the hydrolytic enzyme activity was measured to reach 8500u / ml Put it into the tank, collect the fermented liquid after the tank, use the model SSP450 centrifuge produced by Zhangjiagang Xinhua Chemical Machinery Co., Ltd. to centrifuge at a speed of 1900 rpm, take out the solids and bacterial cells in the fermented liquid, and obtain the crude Lipase enzyme solution.
[0061] Preparation of immobilized lipase: based on the total weight of lipase fermentation broth, first mix 2‰ polyvinyl alcohol, 1‰ Tween 80, 2‰ PVP and 1‰ MgSO 4 The immobilization aid is added into the lipase fermentation broth and mixed evenly. Mix silk (about 1:1.5, the ratio of the weight of the carrier to the crude lipase enzyme liquid) with the crude lipase enzyme liquid, so that the amoun...
Embodiment 2
[0065] Same as the implementation of Example 1, just add 20g of commercially available soybean oil in the 100ml ground mouth Erlenmeyer flask with stopper, then add 12ml (enzyme activity 480U / ml) Candida rugosa 14830 lipase fermentation broth, at 45 ℃ with 120 The reaction was carried out by rev / min insulation shaking. The method for preparing fatty acid in Example 1 was adopted to obtain the crude fatty acid used for participating in the esterification reaction.
[0066] Take 1.39g of the above-mentioned fatty acid, add 291ul of ethanol, add 0.14g of immobilized lipase in Example 1, react at 30°C and 190 rpm for 8 hours, and use the acid value analysis method to measure the final conversion rate of fatty acid ethyl ester up to 81%.
Embodiment 3
[0068] The same implementation as in Example 1, except that 20 g of soybean acidified oil purchased from Qinhuangdao Jinhai Cereals, Oils and Foodstuffs Co., Ltd. was added to a 100 ml ground-mouth conical flask with a stopper, and then 0.4 ml (enzyme activity 5000 U / ml) lipolytic yarrow was added Yeast strain CGMCC NO.2707 crude lipase enzyme solution, and then adopt the method for preparing fatty acid in Example 1 at 40°C, and react with incubation and shaking at 130 rpm to obtain crude fatty acid for participating in the esterification reaction.
[0069] Take 1.40 g of the fatty acid described in Example 1, add 202 ul of methanol, add 0.14 g of the immobilized enzyme described in Example 1, and shake the reaction for 8 hours at 30 ° C and 190 rpm, using the detection method in Example 1 The final fatty acid methyl ester conversion rate reached 82%.
[0070] A certain amount of fatty acid methyl ester obtained is subjected to vacuum distillation at a vacuum degree of 95KPa, ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More