Method for synthesizing biodiesel by two-step catalysis of lipid via Yarrowia lipolytica lipase, and biodiesel obtained thereby

A biodiesel and yeast technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increasing the risk of industrialized production, increasing the intensity of equipment investment, and affecting production efficiency, and achieving increased industrialization feasibility. performance, improved production efficiency, and extended service life
CN101412989AInactive Publication Date: 2009-04-22领先生物农业股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
领先生物农业股份有限公司
Publication Date
2009-04-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a crude lipase enzyme solution of Yarrowia lipolytica microzyme CGMCC No.2707 and a method for synthesizing biological diesel oil by catalyzing the hydrolization and esterification reaction of waste animal and vegetable fat by the immobilized lipase of the crude lipase enzyme solution. A method for preparing the lipase crude enzyme solution and the immobilized enzyme is also provided. The method uses hydrolization and esterification reactions of the oil and fat use lipase as catalysts, but does not use strong acid and strong base. The method has the advantages of mild reaction condition and small pollution. Organic solvent is not used during the entire production process of the fatty acid methyl ester / ethyl ester. The reaction is safe and the efficiency is high.
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Description

【Technical Field】

[0001] The invention belongs to the field of biochemical technology. More specifically, the present invention relates to a method for synthesizing biodiesel from fats and oils using biological enzymes and the obtained diesel. 【Background technique】

[0002] The combustion performance of biodiesel is comparable to that of petrochemical diesel. It is a new type of pollution-free renewable energy. Its research and application have received extensive attention.

[0003] The current method of producing biodiesel is mainly chemical method, which uses high temperature and high pressure, high reaction energy consumption, many side reactions, and strict raw material requirements. Research on the green chemical method of bio-enzymatic synthesis of biodiesel is becoming a hot spot in the biodiesel industry. Enzymatic synthesis of biodiesel generally has many advantages such as mild reaction conditions, fewer side reactions, and no pollutant emissions during the reaction. ...

Claims

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