China rose salt-resistance strain vitro quick-screening method
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A screening method and salt-tolerant technology, applied in the field of agricultural biology, can solve the problems of soil salt stagnation, long breeding cycle of rose varieties, etc., and achieve the effects of good repeatability, increased mutation frequency and survival rate, and stable results.
Inactive Publication Date: 2009-05-06
INST OF QUALITY STANDARD & DETECTION TECH YUNNAN ACAD OF AGRI SCI
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[0007] The purpose of the present invention is to solve the problems that the breeding cycle of rose varieties is long, it takes 2 years for hybridization and selection to
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Embodiment 1
[0082] Example 1: In Vitro Rapid Screening of Salt-tolerant Strains of Rose Variety Versilia
[0083] The material used in this embodiment is Chinese rose variety Versilia (Versilia), and three treatments were performed, each treatment was repeated twice. Treatment 1 is carried out according to the following steps, treatment 2 and treatment 3 are the same as treatment 1 except for the measures listed in Table 1. Processing 1 is as follows:
[0084] 1. Selection and sterilization of the initial explants, select the year-old new shoots that grow robustly, have no damage by diseases and insect pests and virus symptoms, cut into stems containing (1 to 2) lateral buds in each section, cut off the leaves and put them in Soak in water with a small amount of washing powder for 5 minutes, rinse with tap water, and rinse with 0.2% mercuric chloride (HgCl 2 ) solution for 20 min, 2% sodium hypochlorite solution for 25 min, and after sterile water washing for 3 times, germination induct...
Embodiment 2
[0101] Embodiment 2: In vitro rapid screening of rose variety Tinete salt-tolerant strain
[0102] The material used in this embodiment is the Chinese rose variety Tinete. Except that the variety of the material used is different from that of Example 1, three treatments are also performed, and each treatment is repeated twice. Processing 1, processing 2, and processing 3 are the same as processing 1, processing 2, and processing 3 of Embodiment 1, and will not be repeated here.
[0103] Description of comparison settings
[0104] Control 1:
[0105] Above-mentioned implementation 1 and each treatment of implementation 2 all set 2 contrasts simultaneously, and the Chinese rose varieties used are also corresponding correspondingly, and contrast 1 is that the physical mutagenesis treatment of step 7 is not carried out, and the leaves induced by steps 5 and 6 are directly regenerated. Subsequent salt tolerance screening, the same treatment code represents except not 60 Except f...
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Abstract
The invention discloses an excised quick screening method for the salt-resistant strains of a China rose, which belongs to the field of agriculture biotechnology. The invention consists of 13 steps of the selecting and sterilizing of an initial explant, sprout inducing, propagation culture, the continuous subculture of propagated seedlings, the direct regeneration of vanes, the indirection regeneration of vanes, physical mutagenesis, mutagenesis resuming and culturing, the excised quick screening of the salt-resistant strains, the quick propagation and re-screening of the salt-resistant strains, resuming and culturing and seedling strengthening, rootage and transplanting. In the invention, the callus or the directly regenerated adventitious bud of the vane of the China rose is used as a processing material to mainly research the four key procedures of the excised screening technology, the subculture propagation, the seedling strengthening and the rootage culturing of the salt-resistant strains; the method has good repetitiveness, can effectively eliminate false positive strains, and obtain the salt-resistant strains with stable heredity; and the average total surviving rate of the salt-resistant screening thereof achieves about 18 percent which is improved by about 16 percent than comparison 1 and is improved about 18 percent than comparison 2; and the salt-resistant screening efficiency is greatly improved.
Description
technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for in vitro screening and rapid propagation of rose salt-tolerant strains. Background technique [0002] Rose (Rosa cvs.) is one of the most important cut flower species in the world flower market. Among cut flower auctions in the Netherlands, rose has ranked first for many years, ranking first among the four major cut flowers in the world. At the same time, rose is also the largest cut flower in my country and even in Yunnan Province. It is the top priority of cut flowers and has created a huge economy. benefit. However, in the process of rose cultivation and production, salt tolerance has become one of the key factors restricting the quality and yield of cut roses, and the current cultivation mode of cut roses is mostly protected cultivation, and the production under the continuous cropping mode causes greenhouse soil Salt accumulation is increas...
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