Preparation of enzyme-catalyzed rapid-solidified hydrogel and use thereof

A rapid curing and hydrogel technology, applied in the field of medical biomaterials, can solve the problems of cumbersome preparation steps, unsatisfactory hydrogel mechanical strength, and difficulty in controlling the substitution rate

Inactive Publication Date: 2009-05-27
郭倩 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this type of hydrogel has good biocompatibility, due to the cumbersome preparation steps of the precursor macromolecules, tyramide-substituted hyaluronic acid molecules, and it is difficult

Method used

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  • Preparation of enzyme-catalyzed rapid-solidified hydrogel and use thereof
  • Preparation of enzyme-catalyzed rapid-solidified hydrogel and use thereof
  • Preparation of enzyme-catalyzed rapid-solidified hydrogel and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Synthesis of Difunctional Polyethylene Glycol Monomer Substituted by p-Hydroxyphenylpropionic Acid

[0064]20 g of difunctional polyethylene glycol (Mn=4000, 5 mmol), 3.32 g of p-hydroxyphenylpropionic acid (20 mmol) and 400 mg of p-toluenesulfonic acid were added to a 25 ml one-necked flask at one time, and then 60 ml of toluene was added. Connect the water separator to the flask, connect the spherical condenser to the water separator, and pass the nitrogen protection at the upper end of the condenser. Under rapid magnetic stirring, the temperature of the reaction system is raised to 110-150°C, and the toluene is continuously refluxed to bring out the reaction by-product water, which settles to the lower layer in the water separator. The reaction is generally 4 to 12 hours. After the reaction was complete, the reaction product was precipitated with excess ether. Then adopt the dissolution-precipitation method, dichloromethane as the solvent and anhydrous ether as the...

Embodiment 2

[0066] Synthesis of Tetrafunctional Polyethylene Glycol Monomer Substituted by p-Hydroxyphenylpropionic Acid

[0067] Similar to the method in Example 1. Add 18g of tetrafunctional polyethylene glycol (Mn=36000, 0.5mmol), and 2656g of p-hydroxyphenylpropionic acid (16mmol) and 200mg of p-toluenesulfonic acid into a 250ml single-necked flask at one time, then add 60ml of toluene, and add magnetic force stir bar. Connect a water separator to the single-necked flask, and then connect a spherical condenser to the water separator, and the upper end of the condenser is protected by nitrogen gas. Under rapid magnetic stirring, the temperature of the reaction system is raised to 110-150°C, and the toluene is continuously refluxed to bring out the reaction by-product water, which settles to the lower layer in the water separator. The reaction is generally more than 4 to 12 hours. After the reaction was complete, the reaction product was precipitated with ether. And adopt the dissol...

Embodiment 3

[0069] Synthesis of Polyethylene Glycol-Polypropylene Glycol-Polyethylene Glycol Copolymer (Pluronic F127) Substituted by P-Hydroxyphenylpropionic Acid

[0070] Similar to the method in Example 1. 12.6g of Pluronic F127 (Mn=12600, 1mmol), 1.328g of p-hydroxyphenylpropionic acid (8mmol) and 160mg of p-toluenesulfonic acid were added to a 250ml one-necked flask at one time, and then poured into 60ml of toluene. The single-necked flask is connected as a water separator, and a spherical condenser is connected to the water separator, and the upper end of the condenser is protected by nitrogen gas. After the device is connected, under rapid magnetic stirring, the temperature of the reaction system is raised to 110-150°C, allowing the toluene to generate continuous reflux, taking out the reaction by-product water, and the water settles to the lower layer in the water separator. The reaction is generally more than 4 to 12 hours. After the reaction was completed, the obtained toluene...

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Abstract

The invention relates to a preparation method of a hydrogel rapidly crosslinked by enzyme catalysis, and application thereof. A precursor of the hydrogel consists of a mixture (hereinafter referred to as No. 1 solution) of good-biocompatibility macromonomer aqueous solution with an active phenolic hydroxyl unit and dioxygenase aqueous solution which is taken as a catalyst, and the aqueous solution of the precursor can rapidly react to form a 3D polymer cross-linked network after an oxidant (usually low-concentration oxydol, hereinafter referred to as No. 2 solution) is injected. As the No. 1 solution and the No. 2 solution have low-viscosity characteristic, are suitable for being transported and injected, and can rapidly crosslink to form a gel network, the hydrogel can be used for vascular embolization treatment, cancer treatment, family planning operation blocking agents, injectable tissue engineering scaffold materials, soft tissue fillers of human body, controlled release drug carriers, anti-adhesive membranes used in operations and the like after adding one or a plurality of components with specific properties to the No. 1 solution or the No. 2 solution or the gel network.

Description

technical field [0001] The invention relates to the preparation and application of an enzyme-catalyzed fast-curing hydrogel, which belongs to the field of medical biomaterials. Background technique [0002] An embolic agent used in embolization therapy is a substance placed in a blood vessel to block the flow of blood. At present, embolization has been widely used as a medical method, and its main application fields are: (1) In terms of bleeding control, embolization therapy can control bleeding caused by various reasons in the body. (2) In the treatment of vascular diseases, such as arteriovenous malformations, arteriovenous fistulas, and aneurysms, embolization can be used to treat them. (3) In terms of tumor treatment, embolization for tumor treatment can be divided into preoperative embolization and palliative treatment. (4) In terms of eliminating the function of diseased organs, it is mainly used for embolization of spleen and kidney. In addition, the widespread ado...

Claims

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Application Information

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IPC IPC(8): A61L31/06A61L31/16A61L27/18A61K47/34A61P41/00A61K47/10
Inventor 郭倩张正才
Owner 郭倩
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