Method for preparing vegetable seed active peptide by mixed bacteria solid-state fermentation

Abstract

The invention provides a method for preparing rapeseed active peptide by using mixing bacteria to perform solid state fermentation. The method comprises the following steps: rapeseed pulp obtained by pressing or leaching oil preparation process is crushed to obtain crushed rapeseed pulp; one of bacillus subtilis, lactic acid bacteria or candida utilis is compounded with actinomucor elegans or aspergillus usamii to prepare a fermenting agent; the fermenting agent is injected in the crushed rapeseed pulp for solid state fermentation; a culture medium after solid state fermentation is extracted by adding water, and the residue is removed by separation so as to obtain a coarse extracting solution of the rapeseed active peptide; and the coarse extracting solution is decolored, desalted and dried to obtain the rapeseed active peptide. The method has the advantages of environmental protection and mild reaction conditions, and can fully utilize hydrolytic action and sulfur glycoside degradation action of protease, so the method is low in cost and suitable for industrialized production of the rapeseed active peptide.

Description

technical field [0001] The invention relates to a preparation method of rapeseed active peptide, in particular to a method for preparing rapeseed active peptide through mixed culture of two kinds of microorganisms and solid-state fermentation of rapeseed dregs. Background technique [0002] Rapeseed meal is a by-product of the production of rapeseed oil, which contains 35% to 50% of crude protein, and is an important plant protein resource. Because rapeseed meal contains glucosinolates, erucic acid and other anti-nutritional ingredients, it makes rapeseed cake The application of meal as a vegetable protein resource in the food industry is limited. Rapeseed protein is mainly composed of 12S globulin and 2S or 1.7S albumin. The content of lysine, cystine and methionine is relatively high. The amino acid balance is better than that of soybean protein. It is a high-quality protein. Rapeseed protein is hydrolyzed into polypeptides Finally, it has better biological activity and p...

AI Technical Summary

Problems solved by technology

Chemical method, that is, when acid or alkali hydrolyzes vegetable protein, chloropropanol carcinogens will be produced, and the reaction conditions are severe, which destroys the original configuration of amino acids. At the same time, the discharge of hydrolysis waste liquid will also cause certain environmental pollution.

Enzymatic hydrolysis is currently the main method for the preparation of rapeseed peptides in my country, but because the anti-nutritional factors in rapeseed meal or rapeseed protein isolate cannot be completely removed, which affects the activity of proteases, the degree of hydrolysis is not high, and the hydrolyzate of rapeseed protein The problem of flavor, especially bitterness, has not been fundamentally resolved. At the same time, the sources of enzymes used for the hydrolysis of rapeseed protein are limited, and the cost remains high. Enzymatic preparation of rapeseed peptides is limited.