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Transdermal immune influenza multivalent vaccine and preparation method thereof

A multivalent vaccine and influenza technology, applied in the field of vaccines, can solve problems such as pain, reduce side effects, avoid cross-infection, and simplify the process.

Inactive Publication Date: 2009-06-10
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many disadvantages of injecting vaccines with needles, such as the pain associated with the injection, the need for sterile needles and syringes, the need for trained medical personnel to complete the vaccination, the discomfort caused by the injection, and the potential complications of puncturing the skin with a needle

Method used

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  • Transdermal immune influenza multivalent vaccine and preparation method thereof
  • Transdermal immune influenza multivalent vaccine and preparation method thereof
  • Transdermal immune influenza multivalent vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Chicken embryo culture of influenza virus adopts traditional culture method. Type A influenza virus strains include (subtypes such as H1, H3, H5) and type B influenza virus strains are provided by China CDC and Hualan Biotechnology Co., Ltd.

[0043] For influenza virus culture, inoculate the allantoic cavity of 9-11-day-old SPF chicken embryos, culture at 33-35°C for 48-72h (the culture time varies slightly depending on the virus subtype), freeze the chicken embryos at 4°C overnight, and harvest the chicken embryos The allantoic fluid acquires a large amount of virus.

[0044] Mammalian cells (such as Vero cells, MDCK cells, diploid cells, etc.) culture influenza virus, type A influenza virus strains include (H1, H3, H5 and other subtypes) and type B influenza virus strains are produced by China CDC and Hualan Bio Provided by Technologies Inc.

[0045] Use a microcarrier system (such as Cytodex 1, 3, etc.) to culture in a 100ml WAVE reactor, gradually transition to a...

Embodiment 2

[0074] Example 2: In vivo effect observation of influenza multivalent inactivated / attenuated live vaccine in mice

[0075] 1. Immunization plan, use different dosage forms (transdermal, injection immunization as control) influenza virus vaccine to immunize mice

[0076]① Shave 6-8 week old Balb / c mice with 40# scissors. The shaving process should not cause any damage to the skin. The shaving ranges from the middle of the chest to close to the lower part of the strong back. Let the mice rest for 24h. Mice had previously been tagged for identification and pre-bleeded to obtain pre-immune sera.

[0077] ②Then immunize the mice as follows. Mice were anesthetized with 0.03-0.06 ml of 20 mg / ml xylazine solution and 0.5 ml of 100 mg / ml ketamine; this dose of anesthesia made the mice immobile for about 1 h.

[0078] ③Add the immune solution to the skin of the mouse's hair-free back in the following way: gently place a 1.2cm×1.6cm hollow polystyrene plate on the back of the mouse,...

Embodiment 3

[0096] Example 3: In vivo effect observation of multivalent influenza inactivated / attenuated live vaccine in ferrets

[0097] 1. Immunization plan, using different dosage forms of influenza virus vaccine to immunize ferrets

[0098] ① According to the method of preparing the adjuvant / antigen mixture in Example 1, prepare a dosage form suitable for transdermal immunization of ferrets. The solution can be appropriately diluted with PBS for LT.

[0099] ② Male and female ferrets aged 6-10 weeks, with a body weight of 1-2 kg, were selected for this experiment, and all these animals were seronegative before the experiment. 14 days after immunization, blood was drawn from the jugular vein of ferrets to determine the HI titer.

[0100] ③ 6 groups of ferrets received transdermal immunization with vaccines of different dosage forms, each with 1ml of antigen solution containing adjuvants such as LT, CT or CpG, and the patch was used on the back of the ear or outside the upper limbs, an...

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Abstract

The invention provides a transdermal immunity flu polyvalent vaccine and a preparing method. The transdermal immunity flu polyvalent vaccine comprises transdermal immunity adjuvant, flu polyvalent vaccine antigen, permeation agent and medical dressing. The flu polyvalent inactivation or attenuated live vaccine is differ from prior vaccine immunity approach and adjuvant. A result through permeation purpose immunity to Balb / c mouse, ferret, monkey and human body improves that the transdermal immunity flu polyvalent vaccine can generate IgA and IgG antibody with high valency, namely, can induce immune system and mucosal immune simultaneously, also can be used for immunoprophylaxis to popular flu and highly pathogenic avian influenza.

Description

technical field [0001] The invention relates to a vaccine, in particular to a multivalent influenza vaccine for transdermal immunity. Background technique [0002] Influenza is a disease caused by influenza virus (influenza virus) mainly invading the respiratory system. It is widely prevalent in countries all over the world and is one of the important infectious diseases that endanger human health. Therefore, WHO announced that influenza should be strictly monitored. There were three global influenza pandemics in the 20th century, and the first pandemic alone (1918-1919) caused more than 20 million deaths, more than the total number of deaths in World War I, and the deaths Most of them are young and middle-aged, causing huge social and economic losses. In recent years, the repeated outbreaks of seasonal influenza, the incident of H5N1 subtype highly pathogenic avian influenza virus infecting humans, and the emergence of H7N7 and H9N2 subtype influenza viruses, and the fact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/145A61K39/39A61K9/00A61K9/107A61K9/14A61K9/70A61K47/22A61K47/42A61P31/16
Inventor 杨鹏辉王希良罗德炎段跃强邢丽王金成
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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