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Epidermal growth factor nano lipid vector preparation for skin and preparation method thereof

A technology of epidermal growth factor and nano-lipid carrier, which is applied in liposome delivery, skin diseases, medical preparations containing active ingredients, etc. It can solve the problems of poor stability of hEGF, low absorption and utilization of active ingredients, and transdermal effect of preparations Insufficient and other problems, to achieve good physiological compatibility, improve placement stability, good skin moisturizing effect

Inactive Publication Date: 2009-07-08
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are reports in the literature that hEGF is made into dosage forms such as hydrogels, aqueous solutions, and common emulsions. However, due to the relatively high molecular weight of hEGF, the stability of hEGF in these dosage forms is poor, and the dispersion matrix has no obvious absorption promotion effect, resulting in preparations transdermal. The effect is not good, and the absorption and utilization of active ingredients are very low

Method used

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  • Epidermal growth factor nano lipid vector preparation for skin and preparation method thereof
  • Epidermal growth factor nano lipid vector preparation for skin and preparation method thereof
  • Epidermal growth factor nano lipid vector preparation for skin and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Table 1 shows the ingredients and contents of the epidermal growth factor nano-lipid carrier preparations for skin in Examples 1-4, and also shows the encapsulation efficiency and particle size measurement results of the preparations.

[0051] Table 1 Components and content, encapsulation efficiency, particle size determination results of the preparations of Examples 1-4

[0052]

[0053] ① Preparation of hEGF nano-lipid carrier:

[0054] According to the composition of Example 1 given in Table 1, 0.2 mg of hEGF was weighed and dissolved in 500 μL of pure water to obtain water phase 1; 1.5 g of polyoxyethylene stearate (S-40) and 2 g of glycerin were dissolved in the remaining Measure the pure water to obtain the water phase 2; the lipid material glyceryl palmitostearate 4g, soybean lecithin 2g, and tocopherol 0.05g are dissolved in 3mL of dichloromethane to obtain the oil phase; Slowly add water phase 1 and water phase 2 into the oil phase, stir for 1 min to form col...

Embodiment 2

[0066] According to the composition of Example 2 given in Table 1, weigh 0.5 mg of hEGF and dissolve it in 500 μL of pure water to obtain the water phase 1; dissolve 0.3 g of sodium hyaluronate and 2 g of poloxamer 188 in the remaining amount of pure water , to obtain the water phase 2; the lipid material tripalmitin 6g, MCT 2g, polyoxyethylene (35) castor oil 3.5g were dissolved in 3mL of dichloromethane to obtain the oil phase; under high shear conditions, the water Slowly add phase 1 and water phase 2 into the oil phase, stir for 1 min to form colostrum; place the colostrum in a rotary evaporator, and remove dichloromethane under reduced pressure at 30°C; then, use a homogenizer to homogenize at a pressure of 500 bar, Add 0.01 g of chlorobutanol, 0.1 g of urea, and 0.1 g of vitamin C to obtain hEGF nano lipid carrier preparation for skin.

[0067] The determination results of the content, encapsulation efficiency, average particle size and particle size distribution of the ...

Embodiment 3

[0070] According to the composition of Example 3 given in Table 1, 1.0 mg of mouse EGF was weighed and dissolved in 500 μL of pure water to obtain an aqueous phase 1; chitosan was dissolved in the remainder water containing 1.5% acetic acid to obtain an aqueous phase 2; Dissolve 2.5g of lipid materials oleic acid, 6g of stearic acid, 2g of cetyl alcohol, 3g of Tween80, 1.5g of lecithin, and 1g of laurocaprazine in 3mL of dichloromethane to obtain an oil phase; Slowly add water phase 1 and water phase 2 into the oil phase, stir for 1 min to form colostrum; put the colostrum in a rotary evaporator, remove dichloromethane under reduced pressure at 30°C; then carry out high-pressure homogenization, and add nordihydro Guaiac acid and sorbic acid are used to obtain a mouse EGF nano lipid carrier preparation for skin; and then spray-dried to obtain a spray-dried powder.

[0071] Table 1 shows the content, encapsulation efficiency, average particle size and particle size distribution ...

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Abstract

The invention relates to a nano lipide carrier preparation, specifically a nano lipide carrier preparation which contains epidermal growth factor used on the skin and a preparation method thereof. The nano lipide carrier preparation which contains the epidermal growth factor used on the skin contains 0.02-5mg of epidermal growth factor, 2-20g of lipide material, 0.5-10g of emulsifier, 0.01-2g of antioxidizer, 0.01-1.5g of bacteriostat, 0.02-5g of percutaneous absorption accelerant, 0.3-15g of wetting agent and the rest of pure water based on 100mL of nano lipide carrier preparation. The nano lipide carrier preparation containing the epidermal growth factor has good skin physiological compatibility, can improve the placing stability of the epidermal growth factor, effectively promote the percutaneous absorption of the epidermal growth factor and improve the maintaining effect of the epidermal growth factor for the skin.

Description

technical field [0001] The invention relates to a nano-lipid carrier preparation, more specifically, to a nano-lipid carrier preparation for skin containing epidermal growth factor (EGF) and a preparation method thereof. Background technique [0002] The skin of the human body is easily damaged by the effects of various factors such as the natural environment, the atmosphere, and radiation. Therefore, the skin needs protection and repair of the damaged skin. [0003] Traditional skin care is limited to physical methods such as oil film coverage or moisture retention. Nowadays, with the development of life science, people have mastered the aging of epidermal cells and its biochemical process. Therefore, the concept of skin care began to shift to care at the cellular level. At the same time, with the development of biology and skin physiology, people have discovered many natural highly active substances that have the functions of delaying or inhibiting skin aging and restori...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K38/18A61P17/16
Inventor 甘勇张馨欣甘莉朱春柳
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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