Natural killer cell and cultivation method thereof

A technology of natural killer cells and cells, applied in the field of natural killer cells and their culture, can solve the problems of technical difficulty in separating and purifying NK cells, NK cells cannot be widely used in clinical practice, and it is difficult to avoid T lymphocyte contamination, etc., and achieve fast cell growth. , the effect of low cost and wide application value
CN101481678BActive Publication Date: 2011-04-06UNIV OF SCI & TECH OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
UNIV OF SCI & TECH OF CHINA
Publication Date
2011-04-06

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Abstract

The invention discloses a natural killer cell and a culturing method thereof. The natural killer cell is a human natural killer cell NKG cell of which the preservation number is CGMCC No. 2901. The NKG cell has strong lethality to tumor cells, strong survival ability in vivo, stable quality and strong tumor-resistant reaction. Cells cultured by the method have fast growing speed, large quantity, even quality, high and stable survival rate. The preparation method has easy operation, controllable quality, high yield and low cost, and realizes mass, scale, homogeneous and streamlined production for cell treating products like medicament. The method for culturing NKG cells in a large scale can ensure cell phenotype, purity, activity, cell density, culturing volume and the like to achieve clinical treatment and ensure that the NKG cells can be applied to clinical treatment for malignant tumors and injection for 1 to 5 patients at the same time. Therefore, the cells, the method for preparing cells and the preparation method for cell liquid preparation have wide application value in treating malignant tumors.
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Description

technical field

[0001] The invention relates to a natural killer cell and a culture method thereof. Background technique

[0002] Cellular immunotherapy for tumor treatment entered the clinic earlier than other cell therapy schemes. In the schemes that have been tried, LAK cells, CIK cells, and A-NK cells are all anti-cancer systems dominated by NK cells. This is because natural Killer cells (natural killer, NK) are the main members of innate immunity, and can play an important role in the first line of defense against diseases including malignant tumors. In contrast to cells of the adaptive immune system such as T cells, NK cells can initiate cytotoxic activity without prior stimulation or sensitization, thereby providing an immediate natural response. The advantage of NK cells in killing tumors is manifested in two aspects: direct dissolution and secretion of cytokines, which can kill tumors through both perforin and FAS ligand. NK cells can produce TNF-α, IFN-γ and IL-1...

Claims

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