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Method for preparing optical pure (R)-2-octanol by immobilized cell

A technology of immobilized cells and optics, applied in biochemical equipment and methods, methods based on microorganisms, immobilized on/in organic carriers, etc., can solve the problems of complex product separation and non-reusable cells, and achieve follow-up Simple handling, easy continuous mass production, and mild conditions

Inactive Publication Date: 2009-08-12
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there are few by-products, the yield is greatly improved, and the subsequent treatment process is simplified, but the cells cannot be reused, and the product separation is still too complicated [Huang He et al., Bioprocessing. 2004, 2 (2): 52-55; Hu Jian et al., Bioprocessing. 2005, 3(3): 42-46]

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Insert baker's yeast into the culture medium for routine culture. The composition of the medium is that the components contained in each L of culture solution are in g: glucose 20.0, yeast extract 5.0, peptone 2.0, K 2 HPO 4 0.2, KH 2 PO 4 0.5, MgSO 4 1.0, prepare the bacterial solution, soak the cellulose acetate membrane in the bacterial solution, the temperature of the bacterial solution is 30°C, the pH value is 7.0, the rotation speed of the shaker flask is 200rpm, the bacteria are fixed for 24h, the bacterial solution is filtered off, and washed with normal saline, The immobilized cells were heat-treated for 20 minutes to prepare baker's yeast cells immobilized by polymer membranes.

[0014] The immobilized cells were dissolved in 0.1 mol / L Tris-HCl buffer solution to make the mass 0.15 g / mL, and poured into dichloromethane so that the volume ratio of dichloromethane to buffer solution was 10 / 25. Then, 2-octanone was added in two batches to the mixture of dichl...

Embodiment 2

[0016] Insert baker's yeast into the culture medium for routine culture. The composition of the medium is that the components contained in each L of culture solution are in g: glucose 40.0, yeast extract 4.0, peptone 5.0, K 2 HPO 4 0.2, KH 2 PO 4 0.5, MgSO 4 0.1, prepare the bacterial solution, soak nylon 6 in the bacterial solution, the temperature of the bacterial solution is 28°C, the pH value is 6.0, the rotation speed of the shaker flask is 300rpm, the bacterial strain is fixed for 30h, the bacterial solution is filtered off, and washed with normal saline, the fixed The cells were heat-treated for 50 minutes to prepare baker's yeast cells immobilized by polymer membranes.

[0017] The immobilized cells were dissolved in 0.1 mol / L Tris-HCl buffer solution to make the mass 0.05 g / mL, and poured into petroleum ether so that the volume ratio of petroleum ether to buffer solution was 25 / 20. Then, add 2-octanone to the mixture of n-hexane / buffer solution in four batches to ...

Embodiment 3

[0019] Insert baker's yeast into the culture medium for routine culture. The composition of the medium is that the components contained in each L of culture solution are in g: glucose 30.0, yeast extract 5.0, peptone 8.0, K 2 HPO 4 1.0, KH 2 PO 4 1.0, MgSO 4 0.5, prepare the bacterial solution, soak the polycaprolactam membrane in the bacterial solution, the temperature of the bacterial solution is 28°C, the pH value is 7.0, the rotation speed of the shaker flask is 180rpm, the bacteria are fixed for 48h, the bacterial solution is filtered off, and washed with normal saline , the immobilized cells were heat-treated for 55 minutes to prepare baker's yeast cells immobilized by polymer membranes.

[0020] The immobilized cells were dissolved in 0.1 mol / L Tris-HCl buffer to make the mass 0.1 g / mL, and poured into dichloromethane so that the volume ratio of dichloromethane to buffer solution was 30 / 5. Then 2-octanone was added in three batches to the mixture of dichloromethane / ...

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Abstract

The invention discloses a method for preparing high optical pure (R)-2-octanol by a microbiological method, and a microorganism thereof. An immobilized-cell synthesis method of the invention has the advantages that: 1, compared with free cells, immobilized cells can greatly improve the physical-chemical stability of enzyme, have excellent catalytic effect and can be repeatedly used; 2, the immobilized cells prepared from a polymeric membrane carrier are easy to separate from reaction products, and catalysts can be repeatedly used; 3, subsequent treatment is simple and convenient for continuous large-scale production; 4, as reaction is carried out in two phases of organic solvent / buffer solution, the toxic action of the organic solvent on the cells can be reduced, and the catalytic activity of the organic solvent is improved; 5, the preparation of the optical pure (R)-2-octanol through the immobilized yeast cells is simple in process, mild in condition, short in reaction time, nearly free from byproducts and up to 90.25 percent e.e. in optical purity; and 6, the method greatly reduces environmental pollution.

Description

technical field [0001] A method for preparing optically pure (R)-2-octanol by microorganisms belongs to the technical field of microorganisms asymmetrically catalyzing prochiral compounds to synthesize optically pure compounds. Background technique [0002] Optically pure 2-octanol, including (R)-2-octanol and (S)-2-octanol, is not only a synthetic steroid, vitamin E, and insect pheromones (bioinsecticides) and other optically active drugs and pesticides It is an important intermediate and an important chiral material for the preparation of high-performance liquid crystals and liquid crystal devices. It is currently known that optically pure 2-octanol can be prepared by chemical, enzymatic and cellular methods. Conventionally, optically pure 2-octanol is prepared by chemical resolution, but the catalyst (strichnine) is expensive and toxic, the operation is complex, difficult, and the yield is low, causing environmental pollution [Ma Hongyi, Henan Chemical Industry. 2004, 8...

Claims

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Application Information

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IPC IPC(8): C12P7/04C12N11/12C12N11/08C12R1/865
Inventor 曾虹燕夏葵姜和孟娟王橙杨常桥时梦洁
Owner XIANGTAN UNIV
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