Reagent box for detecting nature of third type hepatitis virus antibody by using chemiluminescence method

A technology of hepatitis C virus and chemiluminescence, which is applied in chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, etc. It can solve problems such as strict technical requirements, cumbersome operation, and false positives, and achieve linearity The effect of wide range, easy operation and low production cost

Inactive Publication Date: 2009-10-07
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

Among them, the virus gene detection system adopts RT-PCR method, which can detect the concentration of plasma virus particles, but it is difficult to be widely

Method used

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Embodiment Construction

[0020] The hepatitis C virus antibody chemiluminescence qualitative detection kit described in the present invention comprises the hepatitis C virus structural region Core and the nonstructural region NS coated 3 , NS 4 , NS 5 The 96-well opaque polystyrene white microwell plate of the fusion gene recombinant antigen, the working concentration of horseradish peroxidase-labeled mouse anti-human IgG is 1.0mg / ml, the components are luminol 0.15mM, hydroxycoumarin 0.59 mM, gallic acid 0.35mM, Tris-Hcl buffer 0.2M, pH 9.4 luminescent substrate A liquid and components are amino acid oxidase 0.85mM, Tween-200.8% v / v, DTPA (diethylenetriaminepentaacetic acid ) 0.5mM, vitamin C 0.12mM, acetic acid-acetate buffer 0.2M, pH 6.5 luminescent substrate B solution.

[0021] The use procedure of kit of the present invention is as follows:

[0022] Preparation before experiment

[0023] 1. Return all detection reagents and serum samples to room temperature 18-25°C (about 30 minutes);

[00...

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Abstract

The present invention discloses a reagent box for detecting nature of third type hepatitis virus antibody by using chemiluminescence method which includes an immunoreaction titration micropore plate and a detecting reagent, the immunoreaction titration micropore plate is a non-transparent polystyrol micropore plate; the detecting reagent includes third type hepatitis viruse antigen, enzyme label antihuman IgG, irradiancy substrates A liquor and irradiancy substrates B liquor. The reagent box uses chemiluminescence immune analysis method for detecting third type hepatitis virus antibody, the irradiancy substrate reacts chemically and releases mass energy by using enzyme catalysis irradiancy substrates for generating an excitation state intermediate body. When the excitation state intermediate body returns to a stable basic state, the excitation state intermediate body can transmit light quantum, utilize an irradiancy signal measurement instrumentation for measuring light quantum yield, the light quantum yield has a direct proportion with a waited-measured substance amount in sample. The method has high sensitivity, high precision and wide linear range; the reagent box has low manufacturing cost which can reduce patient burden and satisfy clinical requirement.

Description

technical field [0001] The invention relates to a kit for clinical blood immune detection, in particular to a chemiluminescence qualitative detection kit for hepatitis C virus antibody. Background technique [0002] Hepatitis C (HCV) is a global epidemic and is the main cause of end-stage liver disease in Europe, America, Japan and other countries. According to the statistics of the World Health Organization, the global HCV infection rate is about 3%. The source of infection of hepatitis C is mainly acute clinical type and asymptomatic subclinical patients, chronic patients and virus carriers. HCV is mainly transmitted through blood, mainly through blood transfusion and blood products, and through damaged skin and mucous membranes. According to the serological epidemiological survey data in my country, the positive rate of anti-HCV in the general population is 3.2%, and the positive rate of anti-HCV gradually increases with age, from 2.0% in the 1-year-old group to 3.9% in...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/545G01N33/52G01N21/76
Inventor 李桂林刘工程付光宇吴学炜陆莹
Owner AUTOBIO DIAGNOSTICS CO LTD
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