Fast check paper of Staphylococcus aureus causing food poisoning

A staphylococcus, golden yellow technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of unguaranteed reagent quality, time-consuming detection process, affecting confirmation work, etc., to achieve satisfactory clinical application effect, high detection rate, The effect of simple detection methods

Inactive Publication Date: 2009-10-28
于维森
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  • Application Information

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Problems solved by technology

The whole detection process takes a long time, the traditional biochemical identification test cycle is long, the operation is cumbersome, generally takes 5-6 days, the sensitivity is not high, and the quality of the reagents cannot be guaranteed, which seriously affects the confirmation of pathogenic bacteria and the selection on the medium for selective isolation and culture. Suspicious colonies n

Method used

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Embodiment Construction

[0013] Below in conjunction with embodiment the present invention is described in further detail:

[0014] Configure according to the data listed in the following table (grams per liter of distilled water) and the steps described:

[0015] Recipe one:

[0016] Tryptone 10.0-12.0; beef extract 5.0-5.5; yeast extract 1.0-1.5; glycine 10-14; sodium pyruvate 10-12; lithium chloride 5.0-5.5; egg yolk tellurite enrichment agent 50-60mL ;Agarose 0.08-0.12; Sodium Thiosulfate 0.3-0.5; 4% Tetrazole Indicator 2.3-2.8mL; 4% Toluidine Blue 2.3-2.8mL; Mannitol 10-12; Dipotassium Hydrogen Phosphate 5.0-5.5; Rabbit plasma 3.0-4.0mL; bovine fibrinogen 0.5-0.6; add distilled water to 1000mL, adjust pH to 7.1.

[0017]

[0018] Recipe 2:

[0019] Tryptone 11.0-12.0; beef extract 5.2-5.5; yeast extract 1.3-1.5; glycine 12-14; sodium pyruvate 11-12; lithium chloride 5.2-5.5; egg yolk tellurite enrichment agent 55-60mL...

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Abstract

The invention relates to fast check paper of Staphylococcus aureus causing food poisoning, in particular to a formula of a fast check reagent of food poisoning caused by the intestinotoxin of toxin-production Staphylococcus aureus and a preparation method thereof. The invention breaks through a traditional sectional check means and utilizes the characteristics of heat-resisting desoxyribonucleic acid and plasma-coagulase, the check diagnosis process is shortened to 8-12 hours, and the identification accuracy is larger than 97 percent. The fast check paper has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check paper has an application value, has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines, and has an obvious advantage in the application of rapidly identifying and diagnosing toxin-production Staphylococcus aureus which is a food poisoning pathogenic bacterium.

Description

technical field [0001] The invention relates to a rapid detection paper sheet for food poisoning caused by Staphylococcus aureus. Staphylococcus aureus is an important pathogen that causes purulent infection in humans and animals, and is also one of the common pathogens that cause food poisoning in humans. Food poisoning caused by Staphylococcus aureus is very common in all countries in the world. Especially in regions such as North America and Europe, the incidence rate is higher. In the above-mentioned countries, there are cases of food poisoning caused by Staphylococcus aureus every year, second only to Salmonella, and the cases of bacterial food poisoning rank 2~3, and the economic losses caused by this are also quite heavy. Cases of food poisoning caused by Staphylococcus aureus in my country are also reported from time to time, so all countries in the world have listed Staphylococcus aureus as a statutory inspection item for food hygiene. The method currently adopted b...

Claims

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Application Information

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IPC IPC(8): G01N33/52
Inventor 于维森
Owner 于维森
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