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Method for preparing chloramphenicol molecularly imprinted polymeric microspheres

A technology of molecular imprinting and chloramphenicol, which is applied in the field of polymer microsphere preparation, can solve problems such as easy agglomeration and irregularity of molecularly imprinted polymer particles, so as to increase the specific surface area, avoid non-selective adsorption, and improve adsorption Effect

Active Publication Date: 2009-12-02
BEIJING PURKINJE GENERAL INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to solve the problem that the molecularly imprinted polymer particles obtained by the bulk polymerization method are irregular and easy to agglomerate, and a method for preparing chloramphenicol molecularly imprinted polymer microspheres is provided

Method used

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  • Method for preparing chloramphenicol molecularly imprinted polymeric microspheres
  • Method for preparing chloramphenicol molecularly imprinted polymeric microspheres

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specific Embodiment approach 1

[0010] Embodiment 1: The preparation method of chloramphenicol molecularly imprinted polymer microspheres in this embodiment is as follows: 1. Dissolve methacrylic acid and chloramphenicol in acetonitrile according to the molar ratio of 2:1, wherein chloramphenicol and chloramphenicol The ratio of acetonitrile is 1mmol: 100mL, then shake for 5h under the condition of shaking frequency of 110Hz, then add ethylene glycol dimethacrylate and azobisisobutyronitrile to obtain a mixture, the mixture is shaken at an ultrasonic frequency of 40KHz, ultrasonic Ultrasonic treatment for 5 minutes at a power of 150W, then filled with nitrogen to remove oxygen and then sealed; 2. Heat the sealed mixture in a constant temperature water bath at 60°C for 24 hours, then cool, centrifuge, discard the supernatant to obtain the precipitate, and then The precipitate is naturally dried; 3, the precipitate obtained in step 2 is washed with 10% acetic acid methanol solution to remove chloramphenicol unt...

specific Embodiment approach 2

[0013] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 3, the precipitate obtained in step 2 is washed with acetic acid methanol solution in a Soxhlet extractor. Others are the same as the first embodiment.

specific Embodiment approach 3

[0014] Specific embodiment three: utilize precipitation polymerization method to prepare the blank polymer that does not add chloramphenicol template molecule in the present embodiment and the preparation method is as follows: 1, 2mmol methacrylic acid is dissolved in 100mL acetonitrile, then in the shaking frequency is 110Hz Shake for 5 hours under the conditions, then add ethylene glycol dimethacrylate and azobisisobutyronitrile to obtain a mixture, and ultrasonically treat the mixture for 5 minutes at an ultrasonic frequency of 40KHz and an ultrasonic power of 150W, and then fill it with nitrogen to remove oxygen Seal; 2. Heat the sealed mixture in a constant temperature water bath at 60°C for 24 hours, then cool, centrifuge, and discard the supernatant to obtain a precipitate, and then dry the precipitate naturally; 3. Use the precipitate obtained in step 2 with Wash with 10% acetic acid methanol solution to remove residual monomers and cross-linking agents, then wash with ...

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Abstract

The invention discloses a method for preparing chloramphenicol molecularly imprinted polymeric microspheres, and relates to a method for preparing polymeric microspheres. The method solves the problems that a molecularly imprinted polymer obtained by adopting a mass polymerization method has irregular particles and is easy to agglomerate. The method comprises the following steps of: 1, dissolving methacrylic acid and chloramphenicol into acetonitrile, adding ethylene glycol dimethacrylate and zaodiisobutyronitrile after the oscillation to obtain a mixture, performing ultrasonic treatment on the mixture, and then sealing the mixture after nitrogen aeration and oxygen removal; 2, performing heating polymerization on the sealed mixture for hours, cooling and centrifugating the mixture, and then naturally drying a precipitate; and 3, removing the chloramphenicol from the precipitate, washing the precipitate with methanol for 5h, and drying the precipitate to obtain the chloramphenicol molecularly imprinted polymeric microspheres. The chloramphenicol molecularly imprinted polymeric microspheres obtained by the method have regular particle shapes, uniform size, and no agglomeration phenomenon, and increase the specific surface area of the chloramphenicol molecularly imprinted polymeric microspheres, thereby improving the adsorptivity.

Description

technical field [0001] The invention relates to a preparation method of polymer microspheres. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is a broad-spectrum antibiotic with strong bactericidal power. Due to its excellent antibacterial properties, stable drug properties, and low price, it is widely used as a feed additive to treat bacterial diseases. However, studies have found that CAP is highly toxic to bone marrow and neonates, and can easily cause serious toxic side effects such as human blood poisoning and irreversible aplastic anemia, posing a huge potential threat to human health. [0003] Trace CAP detection methods (microbial method, immunoassay, chromatography, chromatography mass spectrometry, supercritical fluid chromatography, etc.) have been relatively mature. However, due to the low concentration of CAP residues in the sample, complex matrix components, and cumbersome steps in conventional sample pretreatment methods, the effective de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F222/14C08F2/14C08J9/26
Inventor 王静佘永新王荣艳王培龙
Owner BEIJING PURKINJE GENERAL INSTR
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