Cocoon sericin layer alcohol soluble substance and preparation method thereof

A technology of alcohol-soluble matter and cocoon silk, which is applied in the field of silkworm cocoon sericin layer alcohol-soluble matter and its preparation, can solve the problems of impracticality, etc., and achieve good application prospects, obvious social benefits, and low cost effects

Inactive Publication Date: 2009-12-16
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method of directly extracting silk can only obtain a small amount of samples such as quercetin, which is generally only used for qualitative analysis and is not practical.
However, there are few reports about extracting a large amount of active substances such as non-sericin components in the cocoon layer, and carrying out independent research and analysis on its biological activity.

Method used

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  • Cocoon sericin layer alcohol soluble substance and preparation method thereof
  • Cocoon sericin layer alcohol soluble substance and preparation method thereof
  • Cocoon sericin layer alcohol soluble substance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation method of silkworm cocoon sericin layer alcohol soluble product comprises the following steps:

[0029] (1) First remove the impurities or impurities of cocoon shells, cocoon clothing, and waste silk, then soak in water or electrolyzed water overnight, and then place it in a high-temperature and high-pressure cooker at 120°C for 2 hours, and the silk degumming solution obtained therefrom,

[0030] (2) Filter the above-mentioned silk degumming liquid to remove water-insoluble matter and residue, and then concentrate it with a rotary concentrator to obtain a sericin concentrate; add an equal volume of organic solvent to vibrate and extract for 2-10 hours; static layering or centrifugation (5000rpm×10min ) after separation, collect the precipitate, then add an organic solvent for extraction; repeat the above operation 3 to 4 times, completely elute the non-sericin components adsorbed on the sericin, and combine the supernatant, and the organic solvent is eth...

Embodiment 2

[0034] Determination of Amino Acids and Total Flavonoids

[0035] Carry out amino acid and total flavonoids content analysis to the silkworm cocoon sericin layer ethanol soluble matter that embodiment one obtains:

[0036] 1. Amino acid

[0037] The silkworm cocoon layer is degummed in 120°C water under high temperature and high pressure. Some sericin peptides will definitely be degraded or hydrolyzed into small molecule sericin peptides (oligomeric peptides) or free amino acids. The total amount of amino acids in the dissolved matter was analyzed. Weigh a certain amount of alcohol-soluble product with 6.0mol / L hydrochloric acid and hydrolyze it at 110°C for 24 hours. After the hydrochloric acid in the alcohol-soluble product hydrolyzate is completely volatilized and removed, use an automatic amino acid analyzer to determine the total amount and composition of amino acids. The types and compositions of amino acids in the ethanol solubles of five silkworm cocoon sericin layer...

Embodiment 3

[0046] Antioxidant activity test

[0047] Using a 96-well plate, add 80 μL of sample or ethanol solution (control group) to the hole, and then add 0.4 mM (ie 0.4 mMol / L) 1,1-diphenyl 1,2-picric acyl radical (DPPH) ethanol solution 80 μL, mixed thoroughly and left to react for 30 minutes, and then measured its absorbance on a spectrophotometer with a wavelength of 550 nm. Taking ethanol as 100% blank sample, calculate the antioxidant activity (clearance rate %) of the sericin sample. Take the value of the standard reaction absorbance without adding silkworm cocoon sericin layer alcohol solubles as 100% after removing the blank, record the absorbance value of adding various alcohol solubles and calculate the half value of inhibition IC 50 . Table 3 and Table 4 respectively show the inhibition half value of DPPH activity of silkworm cocoon sericin layer alcohol solubles and high-purity sericin protein. It can be seen from the table that the antioxidant activity of silkworm coco...

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Abstract

The invention discloses a preparation method of a cocoon sericin layer alcohol soluble substance, comprising the following steps: wetting clean cocoon shell, cocoon outer floss, waste silk or raw silk in water and carrying out degumming treatment for 1-5h under the conditions that the temperature is 110-140 DEG C and the pressure is 0.1-0.26Mpa to obtain silk degumming liquid; filtering and concentrating the silk degumming liquid to obtain concentrated sericin liquid and then adding an organic solvent for extraction; collecting supernatant and repeatedly extracting lower precipitate with the organic solvent; carrying out spray drying or vacuum freeze drying after concentrating combined supernatant to prepare powder, namely the cocoon sericin layer alcohol soluble substance; wherein, the volume ratio of the concentrated sericin liquid to the organic solvent is 1:1-9. The non-sericin component obtained by the invention has antioxidant activity, antityrosinase activity and alpha-glucosidase inhibition activity, and has wide application prospects in such aspects as cosmetics, anti-aging health care products, hypoglycemic drugs and the like.

Description

technical field [0001] The invention relates to an extract of silkworm cocoon sericin layer, in particular to an alcohol soluble product of silkworm cocoon sericin layer and a preparation method thereof. Background technique [0002] Silk is mainly composed of silk fibers accounting for 70% of the total amount and 30% of the sericin layer covering the silk fibers. In the sericin layer: sericin protein accounts for about 25% of the total cocoon layer, and the remaining 5% is non-sericin components, such as carbohydrates, waxes and pigments. Silk fiber and sericin are secreted by the rear and middle silk gland cells of the silkworm respectively, while the non-sericin component comes from mulberry leaves, which are eaten by silkworms. It is digested and absorbed by the midgut, enters the blood, and finally reaches the silk glands to mix with sericin. When silkworms spin cocoons, this gelatinous mixture is coated on the outside of silk fibers to protect and glue the fibers, ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K35/64A61P3/10A61Q19/00A23L1/30A23L33/10
Inventor 张雨青周丽霞马永雷卢立真周珍祯李琳贺天珍阎海波
Owner SUZHOU UNIV
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