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Thrombolytic active substance extracted from Guizhou fermented blank bean and product thereof

A technology of active substances and extracts, applied in the field of thrombolytic active substances and products, can solve problems such as loss, quality decline, mildew, etc., and achieve the effects of huge market potential, obvious thrombolytic effect, and good development prospects

Inactive Publication Date: 2010-01-13
贵州秀生堂医药生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional processing process, if the craftsmanship is not paid attention to, the quality will decline, and even mildew will cause losses.
Guizhou Douchi belongs to the bacterial type and is rich in nutrition, but there is no report on the research on the active substances of this food

Method used

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  • Thrombolytic active substance extracted from Guizhou fermented blank bean and product thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0011] Research on the preparation process of Guizhou Douchi: A single colony producing Douchi active substances was isolated and screened from Douchi commercially available in Guiyang City, Guizhou Province by the method of streaking on a plate, and subcultured as the test strain. Take soybean peptone 1%, beef extract 0.5%, NaCl 0.5%, NaH 2 PO 4 0.1%; add distilled water to 6ml (2% inoculum size), adjust pH to 9.0. Autoclave at 121°C for 30min. Introduce the above-mentioned isolated bacterial strains into the culture medium on the ultra-clean workbench, put them into a constant temperature incubator and cultivate them for 24 hours, and obtain the seed solution. Soak 300g of soybeans for 1-10 hours, cook at 110°C for 10-50 minutes, sprinkle the seed solution evenly on the cooked soybeans after cooling, place in a biochemical incubator, and incubate at 37°C for 48 hours to obtain the finished soybean meal.

experiment example 2

[0013] Study on the extraction and separation method of active components in Guizhou Douchi: The extraction solution obtained by leaching Douchi with physiological saline was refrigerated and centrifuged at 5000 rpm for 5-30 minutes. Take the supernatant for fractional precipitation, add ammonium sulfate to 30%, 45%, 50%, 55%, 70%, 80%, and 85% in turn; The activity of the thrombolytic active ingredient. The diameter of the melting circle is taken as the ordinate, and the salt concentration of 0% to 85% is taken as the abscissa. The optimal salting-out concentration of 55% was obtained, and the active substance of Douchi was isolated.

experiment example 3

[0015] Determination of thrombolytic activity of active ingredients in Guizhou soybean meal: take a sterile petri dish, place it in a horizontal operating table, prepare agarose solution, add fibrinogen solution and thrombin solution to it after cooling, carefully shake and pour into two sterile Bacteria Petri dish, and then stand at room temperature overnight to make a fibrin plate, and punch a number of small holes on it. Taking urokinase as a standard, take a certain amount and add them into the small holes; take a certain amount of the active components obtained in each stage and drop them into the small holes. After being incubated in a constant temperature incubator, the diameters of the dissolution circles of the standard and samples were measured with a vernier caliper, and the thrombolytic potency of the active components obtained at each stage was calculated using the standard curve.

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Abstract

The invention provides a thrombolytic active substance extracted from a Guizhou fermented blank bean and a product thereof. The fermented blank bean is a folk favorite conventional food in Guizhou. The substance is prepared by taking soybeans as a raw material and carrying out microorganism fermentation, and the flavor tastes sweet but smells smelly. People think that the food stimulates the appetite and is favorable for digestion, and the substance is generally edible and a favorite food of people in Guizhou for a long time. The natural thrombolytic active substance extracted from the Guizhou conventional fermented blank bean and the product thereof have obvious thrombolytic activity, can reduce blood viscosity, promote blood circulation, and are used for treating and preventing heart cerebrovascular diseases such as thrombus diseases and the like. The thrombolytic active substance has the biochemistrical characteristics that: molecular is 28KD; the optimal pH is 8.0; the substance is stable below 50 DEG C, and is not stable in the stomach environment; and the substance has the hrombolytic effects of activating plasminogen to be converted into fibrinoclase, and directly dissolving fibrin in the thrombus.

Description

Technical field: [0001] The invention relates to a thrombolytic active substance and a product extracted from Guizhou Douchi. technical background: [0002] Fermented soya bean is a traditional food loved by Guizhou folks. It is made from soybeans and fermented by microorganisms. and favorite foods. According to the different microorganisms involved in koji making, tempeh can be divided into mucormycetes, aspergillus, rhizopus and bacterial types. In the traditional processing process, if the craftsmanship is not paid attention to, the quality will decline, and even mildew will cause losses. Guizhou Douchi belongs to the bacterial type and is rich in nutrition, but there is no report on the research on the active substances of this food. In view of this situation, the research on the active substances of Guizhou Douchi has good development prospects and huge market potential. Invention content: [0003] The purpose of the present invention is to: provide an active subs...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K36/481A61K36/537A61K36/73A61K9/48A61P1/00A61P1/16A61P3/06A61P3/10A61P7/02A61P9/10A61P9/12A61P19/10A61P37/04A61P39/06A61K35/74A61K38/44A61K38/48
Inventor 高秀丽
Owner 贵州秀生堂医药生物有限公司
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